Generate an extensive expressed sequence tag (EST) database from
two different genotypes each of lettuce and sunflower.
Project overview. Library construction and sequencing.
Data processing, storage and analysis.
The Compositae Genome Project
Determine the extent of synteny among sunflower, lettuce and Arabidopsis.
Develop PCR-based markers for high-throughput genetic analyses.
The initial phase of this project is complete. Over 60,000
lettuce and 40,000 sunflower ESTs have been sequenced from multiple cDNA
ESTs have been assembled using the CAP3 program and organized into the Compositae
Genome Project database (CGPDB) http://cgpdb.ucdavis.edu/.
This assembly represents about 19,000 lettuce and 12,000 sunflower unigenes.
was chosen as an efficient tool to manage the data. Custom PHP
http://www.php.net/ and Python
programs were developed with publicly available phpMyAdmin software
to manipulate the data and visualize the assemblies.