• Intro

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  • Credits



  • CGPDB
    UC Davis


    akozik@atgc.org


    Genotyping Technique

    1. DNA isolation from plant individuals

    2. PCR - DNA amplification

    3. Genotyping - analysis of PCR products



    current efficiency of mapping at CGP:

    ~300-400 markers per year

    130 individuals ( 96 x 2 reaction plates) per marker

    ~ 1 marker per day ( > 100 PCR reactions per day )

    1 or 2 large electrophoresis per day

    need ~10 years to map 4,000 genes (final goal - 20,000 genes)

    hard (impossible) to make it more efficient without introducing a totalitarian regime in the lab

    consideration of an alternative high-throughput approach - better solution