Supplemental Data to the paper:
A HIGH-DENSITY, INTEGRATED GENETIC LINKAGE MAP OF LETTUCE (Lactuca spp.)

Truco, M.J.^[1], Antonise, R.^[2], Lavelle, D.^[1], Ochoa, O.^[1], Kozik, A.^[1], Witsenboer, H.^[2], Fort, S.^[1], Jeuken, M.^[3], Kesseli, R. V.^[4], Lindhout, P.^[3], Michelmore, R.W.^[1], Peleman, J.^[2]

[1] The Genome Center and The Department of Plant Sciences, University of California, Davis, CA 95616.
[2] Keygene N.V., 6708 PW Wageningen, The Netherlands
[3] Laboratory Plant Breeding, Graduate School for Experimental Plant Sciences, Wageningen University, PO box 386, 6700 AJ Wageningen, The Netherlands
[4] Department of Biology, University of Massachusetts, Boston, MA 02125

ABSTRACT

An integrated map for lettuce comprising of 2,744 markers was developed from seven intra- and inter-specific mapping populations. A total of 560 markers that segregated in two or more populations were used to align the individual maps. 2,073 AFLP, 152 RFLP, 130 SSR, and 360 RAPD as well as 29 other markers were assigned to nine chromosomal linkage groups that spanned a total of 1,504.5 cM and ranged from 136 to 238 cM. The maximum interval between markers in the integrated map is 43 cM and the mean interval is 0.7 cM. The majority of markers segregated close to Mendelian expectations in the intra-specific crosses. In the two L. saligna x L. sativa inter-specific crosses, a total of 155 and 116 markers in 13 regions exhibited significant segregation distortion. Data visualization tools were developed to curate, display and query the data. The integrated map provides framework markers for mapping ESTs that are being mapped in one core mapping population relative to phenotypes that segregate in other populations. It also provides large numbers of markers for marker assisted selection, candidate gene identification, and studies of genome evolution in the Compositae.

Genetic Map Browser

Information about integrated and individual maps:

Map ID	Map Description	Raw Scores	Map
INT	Integrated Map - framework markers	-	INT_FRAME.map
INT_W	Integrated Map - all markers	-	INT_WHOLE.map
DA0F	L. sativa cv. Salinas (crisphead type) x L. serriola (US96UC23) F2 population RIL7:8 population (UCDavis RW Michelmore)	DA0F.loc	DA0F.map
DB0D	L. sativa cv. Calmar (crisphead type) x L. sativa cv. Kordaat (butterhead type) F2 population (UCDavis RW Michelmore)	DB0D.loc	DB0D.map
DB0H	L. sativa cv. Salinas (crisphead type) x L. serriola (US96UC23) F2 population (UCDavis RW Michelmore)	DB0H.loc	DB0H.map
DB0F	L. saligna (UC82US1) x L. sativa cv. Vanguard 75 (crisphead type F2) population (UCDavis RW Michelmore)	DB0F.loc	DB0F.map
DB0T	L. saligna (CGN5271) x L. sativa cv. Olof (butterhead type) F2 population (Wageningen University; Jeuken et al. 2001 Theor Appl Genet 103: 638-647. )	DB0T.loc	DB0T.map
DB9X	L. sativa cv Salad Bowl (leaf type) x L. serriola CGN14263 F2 population (UCDavis RW Michelmore)	DB9X.loc	DB9X.map
RYDER	L. sativa F4 breeding line 87-25-1M x L. sativa F4 breeding line 87-1090M (USDA-ARS Salinas, CA; Waycott et al. 1999 Heredity 82: 245-251)	RYDER.loc	RYDER.map

email: mjtruco@ucdavis.edu - Maria Truco: Map construction
email: akozik@atgc.org - Alex Kozik: Genetic Map Browser