Input_Record	ID	Length	Target	Exclude	Pair_No	Primer_L_Seq	Primer_R_Seq	Primer_L_Pos_Len	Primer_R_Pos_Len	Primer_L_Tm	Primer_R_Tm	Primer_L_GC	Primer_R_GC	Product_Size
1	Contig773	753	119,43 	1,45  299,454 	1	TGAATCCCTACTGATGCTTTCA	TTCTGCACACTTCTGGTTCG	89,22	275,20	59.708	60.025	40.909	50.000	187
1	Contig773	753	119,43 	1,45  299,454 	2	TGAATCCCTACTGATGCTTTCA	CGTCTGCATTCTGCACACTT	89,22	283,20	59.708	60.056	40.909	50.000	195
1	Contig773	753	119,43 	1,45  299,454 	3	TGAATCCCTACTGATGCTTTCA	TTGGACAGACATGGAGACCA	89,22	223,20	59.708	60.088	40.909	50.000	135
1	Contig773	753	119,43 	1,45  299,454 	4	TGAATCCCTACTGATGCTTTCA	CTTTCGAACTGGATGTGGCT	89,22	196,20	59.708	60.255	40.909	50.000	108
1	Contig773	753	119,43 	1,45  299,454 	5	TGAATCCCTACTGATGCTTTCA	CTGCACACTTCTGGTTCGAG	89,22	273,20	59.708	59.616	40.909	55.000	185

2	Contig754	754	277,29 	1,145  399,355 	1	ATGAAGCAGGATGCAGCAG	CGCATTGCATTTGAATTCCT	223,19	373,20	60.112	60.971	52.632	40.000	151
2	Contig754	754	277,29 	1,145  399,355 	2	AATGAAGCAGGATGCAGCAG	CGCATTGCATTTGAATTCCT	222,20	373,20	61.488	60.971	50.000	40.000	152
2	Contig754	754	277,29 	1,145  399,355 	3	ATGAAGCAGGATGCAGCAG	GCATTGCATTTGAATTCCTTC	223,19	372,21	60.112	59.543	52.632	38.095	150
2	Contig754	754	277,29 	1,145  399,355 	4	ATGAAGCAGGATGCAGCAG	TGTAATTGTGTCGTCGCATTG	223,19	387,21	60.112	60.572	52.632	42.857	165
2	Contig754	754	277,29 	1,145  399,355 	5	AGGATGCAGCAGCAACAGA	CGCATTGCATTTGAATTCCT	230,19	373,20	60.727	60.971	52.632	40.000	144

3	Contig1104	629	223,18 	1,135  338,292