New High-Throughput Technologies for Genetic Mapping


DNA chip microarray approach (Affymetrix)


Single-feature polymorphism discovery in the barley transcriptome
by Nils Rostoks, Justin O Borevitz et al., Genome Biology 2005, 6:R54


SFP - Single Feature Polymorphism

The method can find ~2,000 - 5,000 SFPs simultaneously by hybridization of genomic DNA (Arabidopsis) or cDNA (barley) isolated from two different genotypes to 6 - 12 chips