BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208500|gb|BQ134589.1|BQ134589 1091015E05.x1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (452 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g56345.1 RNA pseudouridylate synthase, putative / contains... 65 1e-11 >At1g56345.1 RNA pseudouridylate synthase, putative / contains Pfam profile: PF00849: RNA pseudouridylate synthase Length = 254 Score = 65.5 bits (158), Expect = 1e-11 Identities = 30/48 (62%), Positives = 34/48 (70%) Frame = -3 Query: 447 RRDVKYGGVIEWDGVECEGHALHAESLSFVHPITGLPITLRSPLPPWA 304 R DVKY GV EW+G EGH LHAE LS HP+TG I +R+PLP WA Sbjct: 204 RGDVKYHGVYEWNGRTFEGHELHAECLSLDHPVTGDSIVIRAPLPYWA 251 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,471,715 Number of Sequences: 27288 Number of extensions: 192143 Number of successful extensions: 517 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 510 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 517 length of database: 11,516,596 effective HSP length: 90 effective length of database: 9,060,676 effective search space used: 543640560 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208494|gb|BQ134583.1|BQ134583 1091015D05.x1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (508 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g11800.1 expressed protein / EST gb|F14156 comes from this... 147 4e-36 >At1g11800.1 expressed protein / EST gb|F14156 comes from this gene;supported by full-length cDNA: Ceres:16491. Length = 426 Score = 147 bits (370), Expect = 4e-36 Identities = 66/101 (65%), Positives = 83/101 (81%) Frame = -2 Query: 507 GPFPLQDGWTDAWVELKPGEDGWTYDTKANGMLSGNRKLQKRLDRFVCKLADFKMDSIEM 328 G FPL D W D W LKPG+ G+TYDTKAN MLSGNR LQKRLDR +C+L D+K+ IEM Sbjct: 324 GKFPLPDKWVDVWEVLKPGDLGFTYDTKANPMLSGNRALQKRLDRILCRLDDYKLGGIEM 383 Query: 327 IGKEAIPGVSYFKEKKIRKENQRIELPVFPSDHFGLVLTIT 205 +GKEAIPG+SY KEKK+R + +++ELPV PSDHFGL++T++ Sbjct: 384 VGKEAIPGLSYVKEKKVRGDIKKLELPVLPSDHFGLLVTLS 424 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,122,611 Number of Sequences: 27288 Number of extensions: 258463 Number of successful extensions: 706 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 685 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 706 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 684463600 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208471|gb|BQ134560.1|BQ134560 1091015A04.x1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (443 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g20320.1 expressed protein / ;supported by full-length cD... 114 3e-26 >At3g20320.1 expressed protein / ;supported by full-length cDNA: Ceres:17362. Length = 381 Score = 114 bits (284), Expect = 3e-26 Identities = 54/83 (65%), Positives = 70/83 (84%) Frame = -3 Query: 441 AEEMQPLLSEVNDSDLLKDVETIAKGLADASGDLRRLKSSMLTPENTDLIKQSIFTLIFT 262 AE+ QPLLSE DS LLK+VE + + L AS DLR++ SS++TPENT+LI++SI+TL++T Sbjct: 287 AEDAQPLLSEFRDSGLLKEVECLTRSLTQASDDLRKVNSSIMTPENTELIQKSIYTLVYT 346 Query: 261 LKNIESISSDISGFTGDETTRRN 193 LKN+ESISSDI GFTGDE TR+N Sbjct: 347 LKNVESISSDILGFTGDEATRKN 369 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,465,496 Number of Sequences: 27288 Number of extensions: 160849 Number of successful extensions: 416 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 410 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 416 length of database: 11,516,596 effective HSP length: 90 effective length of database: 9,060,676 effective search space used: 516458532 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208415|gb|BQ134504.1|BQ134504 1091014C01.x1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (391 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g44620.1 protein-tyrosine-phosphatase-like protein / prote... 106 4e-24 At3g44420.1 putative protein / predicted protein, Arabidopsis... 55 1e-08 >At3g44620.1 protein-tyrosine-phosphatase-like protein / protein-tyrosine-phosphatase - Schizosaccharomyces pombe, PIR:A55446 Length = 177 Score = 106 bits (264), Expect = 4e-24 Identities = 49/76 (64%), Positives = 56/76 (73%) Frame = -3 Query: 350 EQYAEDILNSFERWRRKEPLPDSAPNKVKLMCSYCKHHTESEVPDPYYGGPQGFEKVLDL 171 +Q EDIL ++ W+ + P A KVKLMCSYCK H + VPDPYYGG QGFEKVLDL Sbjct: 102 DQNKEDILKAYNVWKARGNFPPDADKKVKLMCSYCKKHNDKFVPDPYYGGAQGFEKVLDL 161 Query: 170 LEDACESLLDSIVANN 123 LEDACESLLDSI A + Sbjct: 162 LEDACESLLDSITAQS 177 >At3g44420.1 putative protein / predicted protein, Arabidopsis thaliana Length = 235 Score = 55.1 bits (131), Expect = 1e-08 Identities = 27/58 (46%), Positives = 33/58 (56%) Frame = -3 Query: 344 YAEDILNSFERWRRKEPLPDSAPNKVKLMCSYCKHHTESEVPDPYYGGPQGFEKVLDL 171 +AEDIL ++ VKLMCSYCK H + V DPYYGG Q FEKV+ + Sbjct: 14 FAEDILKAYN---------------VKLMCSYCKKHNDKFVHDPYYGGAQSFEKVISI 56 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,452,140 Number of Sequences: 27288 Number of extensions: 173139 Number of successful extensions: 534 Number of sequences better than 1.0e-05: 4 Number of HSP's better than 0.0 without gapping: 526 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 534 length of database: 11,516,596 effective HSP length: 88 effective length of database: 9,115,252 effective search space used: 373725332 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208391|gb|BQ134480.1|BQ134480 1091020H03.x1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (503 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g48720.1 putative protein / similar to unknown protein (gb... 76 1e-14 >At5g48720.1 putative protein / similar to unknown protein (gb|AAD20092.1) Length = 286 Score = 75.9 bits (185), Expect = 1e-14 Identities = 37/56 (66%), Positives = 45/56 (80%), Gaps = 6/56 (10%) Frame = -1 Query: 503 QDINQRIRAPPKKA------PEILGTSAFSGKPVIGKTRIRTEGGKGSITILRTKG 354 +DINQ+IR PP K P ++ TSAFSGKPV+GKT+IRTEGGKGSITI+RT+G Sbjct: 231 KDINQKIRNPPAKPKAHIEEPAVIQTSAFSGKPVVGKTKIRTEGGKGSITIMRTRG 286 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,513,667 Number of Sequences: 27288 Number of extensions: 211837 Number of successful extensions: 570 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 557 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 566 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 675457500 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208320|gb|BQ134409.1|BQ134409 1091016H05.y2 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (383 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g10050.1 lipase-like protein / monoglyceride lipase - Mus ... 153 2e-39 >At4g10050.1 lipase-like protein / monoglyceride lipase - Mus musculus, PID:e1184892;supported by full-length cDNA: Ceres:6822. Length = 350 Score = 153 bits (387), Expect(2) = 2e-39 Identities = 68/105 (64%), Positives = 92/105 (86%) Frame = +3 Query: 69 EERRVAIPDTDNVFNAYTTGSEGPVVFCLHGGGYSGFSFALAASQMKDKARVVAMDLRGH 248 +E ++I +D+VF+ Y G+EGPVVFCLHGGGYSG SF++ AS++K+KARVVAMDLRGH Sbjct: 56 KEDDISITGSDDVFHVYMAGNEGPVVFCLHGGGYSGLSFSIVASKIKEKARVVAMDLRGH 115 Query: 249 GKSTTNDDLDLSIETLTNDIIAVIRTMYGDLPPAIILVGHSMGGS 383 GKS + ++L+LS+ET++ND++AVI+ +YGD PPAI+LVGHSMGGS Sbjct: 116 GKSVSENELELSLETMSNDVLAVIKELYGDSPPAIVLVGHSMGGS 160 Score = 24.6 bits (52), Expect(2) = 2e-39 Identities = 7/16 (43%), Positives = 13/16 (80%) Frame = +2 Query: 17 SHSLHKYAPLDWSAYF 64 ++S KY+P++W +YF Sbjct: 39 TNSSEKYSPVEWKSYF 54 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,145,024 Number of Sequences: 27288 Number of extensions: 197900 Number of successful extensions: 586 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 565 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 582 length of database: 11,516,596 effective HSP length: 87 effective length of database: 9,142,540 effective search space used: 365701600 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208269|gb|BQ134358.1|BQ134358 1091016B02.y2 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (523 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g25120.1 putative protein / ATP-dependent DNA helicase Rep... 167 2e-42 >At4g25120.1 putative protein / ATP-dependent DNA helicase Rep, Escherichia coli, PIR1:HJECDR Length = 938 Score = 167 bits (424), Expect = 2e-42 Identities = 90/164 (54%), Positives = 109/164 (65%) Frame = +3 Query: 30 EGIPPPNILAMTFTTAAASEMRERIGTVAGNAVA*DIAISTFHSFCLQLCRTHAEKLGRT 209 +G+ P NILAMTFT AA SEMRERIG AG A DI ISTFHSF LQLCR HA+KL RT Sbjct: 273 KGLLPSNILAMTFTKAATSEMRERIGKSAGKKAAKDITISTFHSFSLQLCRMHADKLQRT 332 Query: 210 SEFIIYGHGQQRRALIETERLLENDKNNGVGDTTNQYDGDIKNSFKDKAKKWQKFITQAK 389 SEF +YGHGQQRRA+IE RL E +K NG + G+ N A K Sbjct: 333 SEFSVYGHGQQRRAIIEAVRLYEEEKKNGSKTSVPCESGEGLNGAGAGA---------GK 383 Query: 390 ASGRTPEDYEKKGDLTGASVLRHYNEILRSCNALDYHDFINSSI 521 ASG++PE K G+ GA +L +YN+IL++C+ALDYHD I+ S+ Sbjct: 384 ASGKSPEQCRKMGNEIGAKILGNYNDILKACDALDYHDLISCSV 427 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,247,776 Number of Sequences: 27288 Number of extensions: 240480 Number of successful extensions: 718 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 705 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 717 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 729494100 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208255|gb|BQ134344.1|BQ134344 1091015H06.y2 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (483 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g37290.1 putative protein / CG14881, Drosophila melanogast... 137 4e-33 >At5g37290.1 putative protein / CG14881, Drosophila melanogaster, EMBL:AE003713;supported by full-length cDNA: Ceres:9887. Length = 180 Score = 137 bits (344), Expect = 4e-33 Identities = 64/82 (78%), Positives = 75/82 (91%) Frame = +3 Query: 237 MSTNAQRQVERTGRSGTPRDQYLQDLVTQFQNATNEESKEKIVANLANFAYDPFNYAFMR 416 M TN QRQ ERTG+ GTPR QYLQ+LV+QFQNAT+EE+KE+IVANLANFAYDP+NY +R Sbjct: 1 MFTNNQRQEERTGKHGTPRLQYLQELVSQFQNATDEETKERIVANLANFAYDPYNYTILR 60 Query: 417 QLNVLELFLDCITEPNERLIEF 482 QLNVLELF+DCITEPNE+L+EF Sbjct: 61 QLNVLELFVDCITEPNEKLVEF 82 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,644,457 Number of Sequences: 27288 Number of extensions: 214376 Number of successful extensions: 620 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 603 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 620 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 623303772 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208228|gb|BQ134317.1|BQ134317 1091015D06.y2 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (554 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g22800.1 unknown protein / ESTs gb|Z34075, gb|Z34835 and g... 173 5e-44 >At1g22800.1 unknown protein / ESTs gb|Z34075, gb|Z34835 and gb|AA404888 come from this gene Length = 355 Score = 173 bits (439), Expect = 5e-44 Identities = 93/171 (54%), Positives = 114/171 (66%), Gaps = 5/171 (2%) Frame = +3 Query: 57 FSSSSAPDG---LDDGGGRVKIFDRDLKRRHRDRAAWAMFEIY-GLVDAVAENLLDRLED 224 FS+ A G +VKIFDRDLKR HRDRAAW + VDAVA+NLLDRLED Sbjct: 34 FSTEGAYGGDGEFQQNSSKVKIFDRDLKRIHRDRAAWLSRQKNDSFVDAVADNLLDRLED 93 Query: 225 CRKAFPSALCXXXXXXXXXXXXXXXXXXXXXIMMDMSADMVKKWRESENATGDGP-ETHF 401 C+K+FP+A C IMMD S DM+K R++++ + D ET + Sbjct: 94 CKKSFPTAFCLGGSLGAVKRLLRGRGGIEKLIMMDTSYDMIKSCRDAQDDSLDNSIETSY 153 Query: 402 VIGDEEFLPIKESSQDLIMSCLGLHWTNDLPGAMIQCRLALQPDGLFLSAI 554 +GDEEFLP+KESS DLI+S LGLHWTNDLPG+MIQC+LAL+PDGLFL+AI Sbjct: 154 FVGDEEFLPVKESSVDLIISSLGLHWTNDLPGSMIQCKLALKPDGLFLAAI 204 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,054,367 Number of Sequences: 27288 Number of extensions: 195473 Number of successful extensions: 763 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 730 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 760 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 817071892 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208215|gb|BQ134304.1|BQ134304 1091015B04.y2 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (329 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g32050.1 putative protein / norbin, Rattus norvegicus, PIR... 104 7e-24 >At4g32050.1 putative protein / norbin, Rattus norvegicus, PIR2:JC5812; supported by cDNA: gi_18086356 Length = 618 Score = 104 bits (260), Expect = 7e-24 Identities = 55/105 (52%), Positives = 75/105 (71%) Frame = +3 Query: 9 SQKESALHDFLRSIPASIWESHIRVGITAILQNRVVSSEKLHALLLAECMMSILGEDWLS 188 S+ LH+ LRS+P + W ++R G+ AILQNRV SEKLHAL+LAE MMSILGE WL Sbjct: 225 SEYSELLHEPLRSMPDNNWADYMRTGVVAILQNRVAPSEKLHALILAENMMSILGEKWLL 284 Query: 189 EDFEVQDNQNVLSVDKFVLLVLESARVEVAVLLNELAYLKYESSR 323 ++ + + D+ +LLVLES+ VE++VLLN+LAY KYE+ + Sbjct: 285 GGVKLPNVD--IPADRCLLLVLESSHVEISVLLNDLAYKKYEAPK 327 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,325,781 Number of Sequences: 27288 Number of extensions: 148714 Number of successful extensions: 402 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 394 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 401 length of database: 11,516,596 effective HSP length: 85 effective length of database: 9,197,116 effective search space used: 220730784 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208173|gb|BQ134262.1|BQ134262 1091014F01.y2 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (636 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g49810.1 methionine S-methyltransferase (gb|AAD49574.1) / ... 257 3e-69 >At5g49810.1 methionine S-methyltransferase (gb|AAD49574.1) / ; supported by cDNA: gi_5733428_gb_AF137380.1_AF137380 Length = 1071 Score = 257 bits (657), Expect(2) = 3e-69 Identities = 131/197 (66%), Positives = 158/197 (79%) Frame = +1 Query: 37 MFSPALAIVDEHLTRHLPKQWLTSLAIEGRADCNHADGTVTVIEAPLLSDLLIELIRKLQ 216 +FSP LAIVDEHLTR LP+ WLTSLAIE + + +D +TVIE+P SDL+IELI+KL+ Sbjct: 464 LFSPRLAIVDEHLTRQLPRSWLTSLAIEDTS-MDKSDDQITVIESPHQSDLMIELIKKLK 522 Query: 217 PQVVVTGMAQFEAITSAAFENLLNVTKDVGSRLFLDIXXXXXXXXXXXXNGVLKYLAGKT 396 PQVVVTGMA FE ITS++F +LL VTK++G RLFLDI NGVLKYLA Sbjct: 523 PQVVVTGMAPFEVITSSSFLHLLEVTKEIGCRLFLDISDHFELSSLPASNGVLKYLAENQ 582 Query: 397 LPSHAAILCGLVKNQVYSDLEVAFAISEDAAVYKALSQTIELLEGHTSLISQHYYGCLFH 576 LPSHAAI+CGLVKN+VYSDLEVAF I+E A+ KALS+T+E+LEGHT++ISQ+YYGCLFH Sbjct: 583 LPSHAAIICGLVKNKVYSDLEVAFVITEVDAIAKALSKTVEVLEGHTAIISQYYYGCLFH 642 Query: 577 ELLAFQIADRHPQQERQ 627 ELLAFQ+ADRH ER+ Sbjct: 643 ELLAFQLADRHAPAERE 659 Score = 21.9 bits (45), Expect(2) = 3e-69 Identities = 10/21 (47%), Positives = 15/21 (70%) Frame = +2 Query: 2 PSRSVAIENALQCSHRRLQLL 64 PSR+VAIE+A + RL ++ Sbjct: 452 PSRAVAIESAFRLFSPRLAIV 472 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,424,449 Number of Sequences: 27288 Number of extensions: 269927 Number of successful extensions: 718 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 700 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 717 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 1035211376 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208162|gb|BQ134251.1|BQ134251 1091014D12.y2 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (624 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g58110.1 putative protein / predicted proteins, Homo sapie... 199 1e-51 >At5g58110.1 putative protein / predicted proteins, Homo sapiens and Drosophila melanogaster; supported by cDNA: gi_18087564 Length = 196 Score = 199 bits (506), Expect = 1e-51 Identities = 95/160 (59%), Positives = 122/160 (75%), Gaps = 1/160 (0%) Frame = +1 Query: 145 SSYRYWVREASGDAAPLPVPRKL-DTAANGNGNPPPVGSVWNQAGTWEEKNLNSWANSRI 321 SSYRYWVREA+ DAAP P+P+KL + + N P +GS+WN+AGTWEEK+L WA R+ Sbjct: 12 SSYRYWVREATSDAAPPPLPQKLSNNDVSLNTAPASLGSLWNRAGTWEEKSLTKWATDRL 71 Query: 322 KDLMGSLGSLAFPTGKASIDEVSKCSGDAFQVIVRNKKRVGYNYELSLRFSGEWLIKEEN 501 K+L+GS+GSL F +GKA I +V++C GDAF V VRNKKRVGY YELSL+ GEW +E Sbjct: 72 KELLGSVGSLQFSSGKAEIIDVNRCVGDAFLVTVRNKKRVGYTYELSLKVEGEWSFEENM 131 Query: 502 KKIKGHLDIPEFSFGKIDDLEVQVRFGDDKGLASDDRTRI 621 KK+KG L+IPEFSFG++DDLEV V+ +DK L+ + RI Sbjct: 132 KKVKGSLEIPEFSFGELDDLEVDVKLSEDKELSQQLKQRI 171 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.317 0.137 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,995,568 Number of Sequences: 27288 Number of extensions: 224500 Number of successful extensions: 692 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 666 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 689 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 999514432 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208139|gb|BQ134228.1|BQ134228 1091014B10.y2 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (598 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g23860.1 putative protein / gene T22C1.1, Caenorhabditis e... 120 4e-28 >At4g23860.1 putative protein / gene T22C1.1, Caenorhabditis elegans, Z75550; supported by cDNA: gi_17064723 Length = 222 Score = 120 bits (302), Expect = 4e-28 Identities = 53/112 (47%), Positives = 76/112 (67%) Frame = +2 Query: 188 QYKSEPFFMSKGWRETLCRCETCFNFYAQRGIAYLIDKEDSIEEYEKIAKQKREMKLEQQ 367 +++ +P F++K WR LCRCE C Y QR ++YL+D ED+I EYEK AK+KR KLE+Q Sbjct: 100 EFEKKPLFLTKNWRNILCRCEKCLEMYKQRKVSYLLDAEDTIVEYEKKAKEKRTEKLEKQ 159 Query: 368 QGAETNFLNSLNHVQKIEMISGINDMKNEFQSFLESFDASKPVTSDDIHSFF 523 +G + LN+L+HV K+E++ GI D ++ Q +ES SK +TS DI F Sbjct: 160 EGEALDLLNNLDHVSKVELLHGIKDFQDGLQGLMESAGPSKAITSADIEQMF 211 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,955,770 Number of Sequences: 27288 Number of extensions: 201555 Number of successful extensions: 698 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 681 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 698 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 930958496 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208102|gb|BQ134191.1|BQ134191 1091013F09.y2 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (524 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g61040.1 unknown protein / ; supported by cDNA: gi_20147192 226 5e-60 >At1g61040.1 unknown protein / ; supported by cDNA: gi_20147192 Length = 643 Score = 226 bits (576), Expect = 5e-60 Identities = 108/172 (62%), Positives = 139/172 (80%), Gaps = 1/172 (0%) Frame = +1 Query: 10 FDDVKSITLRRSKLVKWFMEPFFDDLISGCFVRLGIGKTKNGTPSYRLCIVRNVDAFDPY 189 F+DVK +T+RRSKL KW MEPFF++LI GCFVR+GIG++K+G P YRLC V+NVDA DP Sbjct: 264 FEDVKEVTIRRSKLAKWLMEPFFEELIVGCFVRVGIGRSKSG-PIYRLCWVKNVDATDPD 322 Query: 190 RNYKLENYSTCKYLNVVWDSEANAARWQMTQVSDSPPNEEEFKEWLQAAEKNGARIPTRQ 369 + YKLEN +T KYLNVVW +E +AARWQM +SD P EEE+++W++ E+ R+PT+Q Sbjct: 323 KTYKLENKTTHKYLNVVWGNETSAARWQMAMISDGHPLEEEYRQWIREVERTNGRMPTKQ 382 Query: 370 EVLDKKEAIQNAYNFVYSAATVQQMLQE-KSAVRRPINIAAEKDRLRSELEM 522 ++ +KKEAIQ +FVYSA TV+QMLQE KSA RP+N+AAEKDRLR ELE+ Sbjct: 383 DISEKKEAIQRTNSFVYSAETVKQMLQEKKSASVRPMNVAAEKDRLRKELEI 434 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,992,297 Number of Sequences: 27288 Number of extensions: 266422 Number of successful extensions: 756 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 741 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 754 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 727283772 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|20208097|gb|BQ134186.1|BQ134186 1091013E08.y2 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (244 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g39680.1 hypothetical protein / 99 4e-22 >At4g39680.1 hypothetical protein / Length = 633 Score = 99.0 bits (245), Expect = 4e-22 Identities = 50/78 (64%), Positives = 56/78 (71%) Frame = +3 Query: 9 SQTGPETPKDIFQPAFKRSFGRSDSTASVDSPKERIVPPAEKPATTSLRIDRFVRPFTLK 188 S T TP+ KR F RSDS+ S D PKER+VPP+ K T SLRIDRF+RPFTLK Sbjct: 415 SATPTTTPRST---GLKRDFSRSDSSVSEDGPKERVVPPSPKEPTNSLRIDRFLRPFTLK 471 Query: 189 AVQELLGKTGSVQDFWMD 242 AVQELLGKTG+V FWMD Sbjct: 472 AVQELLGKTGNVTSFWMD 489 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,207,027 Number of Sequences: 27288 Number of extensions: 94938 Number of successful extensions: 317 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 315 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 317 length of database: 11,516,596 effective HSP length: 56 effective length of database: 9,988,468 effective search space used: 239723232 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19822717|gb|BQ048741.1|BQ048741 952023F05.x4 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (596 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g70570.1 expressed protein / ; supported by cDNA: gi_15293... 167 3e-72 >At1g70570.1 expressed protein / ; supported by cDNA: gi_15293216_gb_AY051042.1_ Length = 595 Score = 167 bits (423), Expect(2) = 3e-72 Identities = 80/97 (82%), Positives = 86/97 (88%) Frame = -3 Query: 300 GPPPVADVNSLTHYGEPYDGNTRFFKSTLFVAAVRACYGESCLLHGVEWMPPKGGITEGQ 121 G PPVADV SLTHYGEPYDGNTRFF+STLFVAAVR+C GES LLHGVEWMPPKGG+TE Q Sbjct: 291 GAPPVADVKSLTHYGEPYDGNTRFFRSTLFVAAVRSCCGESSLLHGVEWMPPKGGVTEEQ 350 Query: 120 MLKFMGANIHLSPTQAKILLEDETAGFAYLNLQEACP 10 MLKFMGAN HLS QAK L+EDE AGFAYL+L+EA P Sbjct: 351 MLKFMGANTHLSVQQAKELIEDEKAGFAYLSLREARP 387 Score = 122 bits (305), Expect(2) = 3e-72 Identities = 62/92 (67%), Positives = 75/92 (81%), Gaps = 1/92 (1%) Frame = -1 Query: 596 ILGANGLAEPRYVGQGTGEMRLVGALREVLAGGHLGYEEVQCVLKDILPIGS-SSDSTVV 420 +LG P +VG T EMRLVGALRE+LAGGHLGYEEV+ VL+D+LP+ + S ++ V Sbjct: 198 LLGTGNSVGPTFVGNETREMRLVGALREILAGGHLGYEEVKGVLRDVLPLETEGSLNSGV 257 Query: 419 SQALLAAFLIGQRMNRETDRELKAYCLTFDDE 324 S++LL+AFLIGQRMNRETDRELKAYCL FDDE Sbjct: 258 SESLLSAFLIGQRMNRETDRELKAYCLAFDDE 289 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,739,113 Number of Sequences: 27288 Number of extensions: 303675 Number of successful extensions: 844 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 784 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 842 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 930958496 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19822478|gb|BQ048502.1|BQ048502 952021D02.y2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (532 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g56090.1 putative protein / contains similarity to cytochr... 280 3e-76 >At5g56090.1 putative protein / contains similarity to cytochrome oxidase assembly factor Length = 457 Score = 280 bits (716), Expect = 3e-76 Identities = 125/173 (72%), Positives = 152/173 (87%) Frame = -3 Query: 530 GIRLSALFALGGAQGLIGWWMVKSGLEEPTSEYVQPRVSPYRLATHLTSAFIIYCGILWT 351 G++LS LFALG QG IGWWMVKSGLEEP SEY QPRVSPYRLA HLTSAF IYCG+ WT Sbjct: 210 GVQLSGLFALGAGQGFIGWWMVKSGLEEPPSEYSQPRVSPYRLAAHLTSAFAIYCGLFWT 269 Query: 350 ALSVVMPDPPTGSMSWVNGAAKIRKLAIPVSAVVGITAISGAFVAGNDAGHAYNSFPKMG 171 ALSVVMP+PP S++WV GAAK++KLA+PVS +VGITAISGAFVAGNDAG A+N+FPKMG Sbjct: 270 ALSVVMPEPPAESLAWVRGAAKVKKLALPVSLIVGITAISGAFVAGNDAGRAFNTFPKMG 329 Query: 170 DSWIPEDVFSMEPFVRNFFENTSTVQLNHRILAATTLLSVGGLWLAARKIDMH 12 D+WIP+++F M+P +RNFFENT+TVQL+HR+LA TTL+++G +W RK+D+H Sbjct: 330 DTWIPDNIFEMKPLLRNFFENTATVQLDHRLLATTTLIAIGTMWWFTRKLDIH 382 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,515,819 Number of Sequences: 27288 Number of extensions: 267620 Number of successful extensions: 784 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 749 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 784 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 745241396 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19822463|gb|BQ048487.1|BQ048487 952021B02.y2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (462 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g12550.1 hypothetical protein / predicted by genscan 83 7e-17 >At2g12550.1 hypothetical protein / predicted by genscan Length = 538 Score = 82.8 bits (203), Expect = 7e-17 Identities = 53/126 (42%), Positives = 72/126 (57%), Gaps = 2/126 (1%) Frame = -2 Query: 383 MLRAEVARRVGDGVEPECVGLIFGGRVLKDDPPASLR--EAGLKANAKVLSSLTSPDRXX 210 MLR EVA+R G++PE + LIF G++LKDD A+L + G+K N+K+LS + Sbjct: 1 MLRDEVAKR--SGLDPESIKLIFAGKILKDDGDATLTLSQLGIKENSKILSCRQAAPEEG 58 Query: 209 XXXXXXXXXXXXXXXXXKLVRLWEAAKALSQRHTDGSYLAEEDYNLDLEDQSGQKVMFGS 30 L R+ AA ALS+RH DGS L +DYN++LEDQ G KV FG+ Sbjct: 59 KSIMAEEERARR------LSRVKAAATALSKRHADGS-LPIQDYNIELEDQGGHKVQFGT 111 Query: 29 VDDKKA 12 D+ A Sbjct: 112 ETDQSA 117 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,420,932 Number of Sequences: 27288 Number of extensions: 149271 Number of successful extensions: 711 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 661 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 702 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 560070056 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19822413|gb|BQ048437.1|BQ048437 1091008B10.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (576 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g34640.1 expressed protein / ; supported by full-length cD... 82 3e-16 >At1g34640.1 expressed protein / ; supported by full-length cDNA: Ceres: 10855. Length = 110 Score = 81.6 bits (200), Expect = 3e-16 Identities = 33/84 (39%), Positives = 53/84 (62%) Frame = +1 Query: 286 RKAAATYGFGILAIAGVLLPDWEFFDRDYSQWLTPMPASRRTXXXXXXDREHDVWKFKPY 465 +K TY GI I G+LLPDW+FFDR +S+W P+ A R+ R+ +F+ Y Sbjct: 29 KKTVVTYAVGISLIGGILLPDWDFFDRSFSRWGYPVTAEERSAALA---RKSHPSRFRVY 85 Query: 466 PLRVAMLTTIYGFGLHKWWTYVSS 537 P+R+ + T+YG+ +++WW +VS+ Sbjct: 86 PMRMVLYGTVYGYAVYRWWMFVSN 109 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,964,932 Number of Sequences: 27288 Number of extensions: 150511 Number of successful extensions: 404 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 386 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 403 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 868297828 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19822319|gb|BQ048343.1|BQ048343 1091006G06.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (628 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g21150.1 putative protein / ribophorin II precursor, Homo ... 282 1e-77 >At4g21150.1 putative protein / ribophorin II precursor, Homo sapiens, PIR2:B26168; supported by cDNA: gi_16604453 Length = 691 Score = 282 bits (722), Expect(2) = 1e-77 Identities = 133/193 (68%), Positives = 162/193 (83%) Frame = +1 Query: 49 KLCLSFQLTTPLGHTFKPHQVFLKLRHESKVEHLFVVPGSARQFKIVLDFLGLVEKFYYL 228 KL LSFQLTTPLG+ FKPHQ F KL+HES+VEH+F+V S ++ ++VLDFLGLVEK YYL Sbjct: 463 KLRLSFQLTTPLGNAFKPHQAFFKLKHESQVEHIFLVKTSGKKSELVLDFLGLVEKLYYL 522 Query: 229 SGRYDLELTVGDAVMENSFLRPLGHLELDLPDAPEKAPRPPAQAVDPFSKFGPKAEISHI 408 SG+Y+++LT+GDA MENS L +GH+ELDLP+ PEKA RPP Q+ +P+S++GPKAEISHI Sbjct: 523 SGKYEIQLTIGDASMENSLLSNIGHIELDLPERPEKATRPPLQSTEPYSRYGPKAEISHI 582 Query: 409 FRAPEKRPPKELSLAFTGLTLLPFIGFLIGLVRLGVNLKNFPSLPGPAAFASLFHAGIGA 588 FR PEK P K+LSL F G+ +LPFIGFLIGL RLGVN+K+FPS G A A LFH GIGA Sbjct: 583 FRIPEKLPAKQLSLVFLGVIVLPFIGFLIGLTRLGVNIKSFPSSTGSAISALLFHCGIGA 642 Query: 589 VLMLYVLFWIKLD 627 VL+LYVLFW+KLD Sbjct: 643 VLLLYVLFWLKLD 655 Score = 25.4 bits (54), Expect(2) = 1e-77 Identities = 12/15 (80%), Positives = 12/15 (80%) Frame = +3 Query: 3 LLKDTKVSLSANHLQ 47 L KD VSLSANHLQ Sbjct: 448 LTKDGAVSLSANHLQ 462 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,274,209 Number of Sequences: 27288 Number of extensions: 317338 Number of successful extensions: 1028 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 995 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1027 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 1008438668 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19822297|gb|BQ048321.1|BQ048321 1091006D01.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (617 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g39580.1 unknown protein / 110 6e-25 >At2g39580.1 unknown protein / Length = 1567 Score = 110 bits (275), Expect = 6e-25 Identities = 67/184 (36%), Positives = 106/184 (57%), Gaps = 4/184 (2%) Frame = +1 Query: 58 EWYEHCIREHAAIHAL-ELEKSSS-STDAQTRATFSLIIGYLADHCNLPTRELLSRRFCQ 231 E + HC+REHA + EL+ + S + Q R L+ YL +LP +E LS +F Sbjct: 1334 EHFMHCLREHAVFQLINELQATGEFSINLQMR----LLNSYLDRASSLPVKEPLSWKFIS 1389 Query: 232 N-IKKHRLRQLIDDTIGSVPADSSLINSVLEVCFGPSLLPKSISDVKYLVDFVETVMEAL 408 N +K R+R+L+ + + V ++ ++N VLE GPSL+P+ +S K LVDFVET++ + Sbjct: 1390 NSAEKPRVRKLVTNLLAPVSSELFVVNVVLEAWHGPSLVPEKLSKQKELVDFVETILGLV 1449 Query: 409 PANYRLGLAVGGFVAK-HFTGYGAASTGTRFWASSVLINAIFRAVPVAPESVWLEGAGLL 585 P+NY L L+V + K +S+G FWA L + I A+PVAPE +W+E ++ Sbjct: 1450 PSNYPLALSVSKLLRKEEKQSDSGSSSGIHFWAGLNLASTISCAIPVAPEYIWVEAGEIV 1509 Query: 586 EKLH 597 ++ Sbjct: 1510 SDIN 1513 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,167,687 Number of Sequences: 27288 Number of extensions: 305127 Number of successful extensions: 827 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 803 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 825 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 981665960 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19822280|gb|BQ048304.1|BQ048304 1091005H05.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (559 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g02310.1 eceriferum3 (CER3) / 231 2e-61 >At5g02310.1 eceriferum3 (CER3) / Length = 795 Score = 231 bits (588), Expect = 2e-61 Identities = 109/182 (59%), Positives = 134/182 (72%) Frame = +1 Query: 4 LDLILQDESVHMLALKWSQHFCEEYNPRKYRGTLFSTPAVPFRLMQLPDVYQVLLERYIK 183 +D+IL DE + W QHF EY + + +L TP VPF+LM+LP++YQ LL+R IK Sbjct: 610 IDIILNDELLRSSTQIWLQHFQREYRVNRVKRSLCITPVVPFQLMKLPNLYQDLLQRCIK 669 Query: 184 MQCPDCGSVPDEPALCLLCGKLCSPSWKPCCRTGKCQNHALQCGAGIGIFLLVRKTTILL 363 +C +C V +EP LCLLCG LCSP W PCCR C NHA+ CGAG G+FLL+R+TTILL Sbjct: 670 KRCVNCTKVIEEPVLCLLCGSLCSPIWSPCCRESGCPNHAITCGAGTGVFLLIRRTTILL 729 Query: 364 QRSARLTFWPSLYLDAFGEEDNEMHRGKPLYLSQERYTSLTYLVASHSLDRTSEVLRQTT 543 QR AR + WPS YLD FGEED +M RGK LYL++ERY +LTYLV SH LDR+S+VL QTT Sbjct: 730 QRFARQSPWPSPYLDTFGEEDIDMIRGKRLYLNEERYAALTYLVGSHGLDRSSKVLGQTT 789 Query: 544 IG 549 IG Sbjct: 790 IG 791 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,996,066 Number of Sequences: 27288 Number of extensions: 295016 Number of successful extensions: 765 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 752 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 765 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 826050704 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19822270|gb|BQ048294.1|BQ048294 1091005G06.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (596 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g47570.1 expressed protein / ;supported by full-length cD... 129 1e-30 >At5g47570.1 expressed protein / ;supported by full-length cDNA: Ceres:12344. Length = 125 Score = 129 bits (323), Expect = 1e-30 Identities = 58/93 (62%), Positives = 66/93 (70%) Frame = +2 Query: 116 MGLPVGRHIVPDKPLPVNDELVWDNGTPFPEPCIDRLAPHIGKYEXXXXXXXXXXXXXXX 295 MGLPVG+HIVPDKPL VNDEL+WDNGT FPEPCIDR+A +GKYE Sbjct: 33 MGLPVGKHIVPDKPLSVNDELMWDNGTAFPEPCIDRIADTVGKYEALAWLSGGLGFFVGL 92 Query: 296 XXXXVVNDKASNIPYTPKVYPYDNLRAELGDRP 394 V+NDKAS +P+TP+VYPYDNLR ELG P Sbjct: 93 GLLAVLNDKASKVPFTPRVYPYDNLRVELGGEP 125 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,423,261 Number of Sequences: 27288 Number of extensions: 225293 Number of successful extensions: 720 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 694 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 719 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 930958496 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19822251|gb|BQ048275.1|BQ048275 1091005E09.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (557 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g63440.1 expressed protein / ; supported by cDNA: gi_1622... 300 3e-82 >At5g63440.1 expressed protein / ; supported by cDNA: gi_16226477_gb_AF428410.1_AF428410 Length = 205 Score = 300 bits (769), Expect = 3e-82 Identities = 139/168 (82%), Positives = 159/168 (93%) Frame = +3 Query: 54 MPKRTTHTYSSEDALPEGPESDLFVYYCKHCASHVLITDTQLQKMPKRKTDRAHVLDKAK 233 MPKRTTHTYSSEDA P+GP+SDLFVYYCKHC SHVLITDTQLQKMPKRKTDR++VLDK Sbjct: 1 MPKRTTHTYSSEDAAPDGPDSDLFVYYCKHCGSHVLITDTQLQKMPKRKTDRSNVLDKKT 60 Query: 234 HLSRLNVKEAGKVMLKRGEGKLEKQFRMSCVGCDLFVCYRSEEDLEVAPFIYVIDGALSS 413 HL+RLNV E GKV+LKRGEGK+E+QFRM+C+GC+LFVCYR+EE+LE A FIY++DGALS+ Sbjct: 61 HLARLNVSEGGKVLLKRGEGKMERQFRMNCIGCELFVCYRAEENLETASFIYIVDGALSA 120 Query: 414 VAAETNPHDAPVPPCITQLEGGVVQVAIEVEDRAQRSAVTRVNADDVR 557 VAAETNP DAPVPPCI+QL+GG+VQVAIEVEDRAQRSA+TRVNADDVR Sbjct: 121 VAAETNPQDAPVPPCISQLDGGLVQVAIEVEDRAQRSAITRVNADDVR 168 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,016,676 Number of Sequences: 27288 Number of extensions: 221326 Number of successful extensions: 862 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 792 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 862 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 826050704 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19822138|gb|BQ048162.1|BQ048162 1091003G01.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (522 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g02100.1 hypothetical protein / predicted by genscan; supp... 196 8e-51 >At1g02100.1 hypothetical protein / predicted by genscan; supported by cDNA: gi_18377693 Length = 332 Score = 196 bits (497), Expect = 8e-51 Identities = 95/141 (67%), Positives = 117/141 (82%) Frame = +1 Query: 100 VQATNDDAAVSKLSCVNKGYMKDDYVRXFVRRPTRRAPIINRGYYARWSVLRTLLHQFLN 279 VQATNDDA+ SKLSCV KGYMKDDYV FV+RP RR+PIINRGY++RW+ R L+ QFL Sbjct: 11 VQATNDDASASKLSCVKKGYMKDDYVHLFVKRPVRRSPIINRGYFSRWAAFRKLMSQFLL 70 Query: 280 AGKNSNNDKPKQILSLGAGFDTTFFQLQDEGMAPHCYVELDFKEVTSKKAAIINHYSEMK 459 +G +S KQILSLGAGFDTT+FQL DEG P+ YVELDFKEVTSKKAA+I + S+++ Sbjct: 71 SGTSSK----KQILSLGAGFDTTYFQLLDEGNGPNLYVELDFKEVTSKKAAVIQNSSQLR 126 Query: 460 EKLGSEASISIEKGEVISTHY 522 +KLG+ ASISI++G+V+S HY Sbjct: 127 DKLGANASISIDEGQVLSDHY 147 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,311,988 Number of Sequences: 27288 Number of extensions: 174958 Number of successful extensions: 437 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 431 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 436 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 729494100 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19822099|gb|BQ048123.1|BQ048123 1091003B11.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (501 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g45360.1 F-box protein family / ;supported by full-length... 219 6e-58 >At5g45360.1 F-box protein family / ;supported by full-length cDNA: Ceres:36901. Length = 316 Score = 219 bits (558), Expect = 6e-58 Identities = 101/155 (65%), Positives = 124/155 (79%), Gaps = 1/155 (0%) Frame = +3 Query: 39 GLFSNVPPELFLQIFKFLSSEDLISCALVCRFMNAAASDETLWRRLYCMRWGLA-SNAKF 215 G+F+NVP ELF I KFLSSEDL+SC+LVC+F+N AA+DE+LWRRLYC+RWGL + K Sbjct: 69 GVFTNVPTELFRHILKFLSSEDLVSCSLVCKFLNFAAADESLWRRLYCIRWGLTLPSRKL 128 Query: 216 RECAWKNLYIQRDREDMVEFVRNTPTEFKEYYIQMQAAKRSQAPRPSEVNDDKVMLDKTV 395 RE AWK LYI RD +DM+E VR P++FKEYY+ MQAAKRSQAP PS++ DD+++LD TV Sbjct: 129 RESAWKKLYIDRDEQDMIELVRTCPSDFKEYYVHMQAAKRSQAPLPSQMVDDRIILDNTV 188 Query: 396 ADQVSSWKNSRGLTDEAVKGHSCSGNTCSYTQIGD 500 +QVS WK S+GLTD+AV GH C G CSY QI D Sbjct: 189 LEQVSLWKKSKGLTDKAVTGHICLGTKCSYHQIDD 223 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,605,477 Number of Sequences: 27288 Number of extensions: 218298 Number of successful extensions: 779 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 757 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 778 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 666451400 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19822033|gb|BQ048057.1|BQ048057 1091002A11.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (582 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g17370.1 putative protein / 292 7e-80 >At4g17370.1 putative protein / Length = 364 Score = 292 bits (748), Expect = 7e-80 Identities = 145/194 (74%), Positives = 164/194 (83%) Frame = +1 Query: 1 LCDAIIVSSPNMTHYEILMDIICHREPQHILVEKPLCTTVQDCKKVIEAAKQRPEIVVQV 180 LCD I+VSSPNMTH++ILMDII + +P H+LVEKPLCTTV DCK+V+EAAK+R ++VVQV Sbjct: 77 LCDVIVVSSPNMTHHQILMDIINYSKPHHVLVEKPLCTTVADCKQVLEAAKKRSDMVVQV 136 Query: 181 GLEYRYMPPVAKLIDIVKSGTLGQVRMVAIREHRFPFLVKVNNWNRFNCNSGGTLVEKCC 360 GLEYRYMPPVAKLI+ VK G V+MVAIREHRFPFLVKV N TLVEKCC Sbjct: 137 GLEYRYMPPVAKLIEQVKGRDFGNVKMVAIREHRFPFLVKVG----LNKMGLWTLVEKCC 192 Query: 361 HFFDLMILFAAANPVRVMASGAIDVNHKNEVYDGQVPDIIDNAYVIVEFDNGSRGMLDLC 540 HFFDLM LFA ANPV VMASG +DVNHK+EVY G+VPDIIDNAYVI+EFDNG RGMLDLC Sbjct: 193 HFFDLMRLFAGANPVCVMASGGMDVNHKDEVYGGKVPDIIDNAYVIIEFDNGCRGMLDLC 252 Query: 541 MFAEGSRNKQEICV 582 MFAEGS+N+QEI V Sbjct: 253 MFAEGSKNEQEISV 266 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,936,220 Number of Sequences: 27288 Number of extensions: 272882 Number of successful extensions: 846 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 821 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 845 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 886200876 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19821883|gb|BQ047907.1|BQ047907 952045H02.x2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (678 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g30990.1 putative protein / probable membrane protein YBL0... 96 2e-20 >At4g30990.1 putative protein / probable membrane protein YBL004w, yeast, PIR2:S45734 Length = 2138 Score = 95.9 bits (237), Expect = 2e-20 Identities = 67/180 (37%), Positives = 104/180 (57%), Gaps = 11/180 (6%) Frame = +3 Query: 12 NLVPAIFSILTVKTASGSITTYALEFIENXXXXXXXXXXXXEHLVKKVVLQHMDVLIHSL 191 +LVP IFSILTV TAS +I + AL+FIEN E++++ V +++ LI+SL Sbjct: 1159 SLVPDIFSILTVTTASEAIKSSALKFIENLLCLDNVLGED-ENMIRGFVDPYIEALINSL 1217 Query: 192 HD-FVNHRKELNRRSGTWLGQRELRLFKLLLNYITDPSTADKFIDLILPFFSK--KD--- 353 H F+ L R+S + G+RE+++ KLL + D S K++D++L F +K KD Sbjct: 1218 HSLFIGDI--LKRKSVKYHGEREIKILKLLSKRMQDRSHVMKYLDVLLSFLNKSVKDPGM 1275 Query: 354 -----LNSDDCLEALCVVRGIIQNLRCKVSEKVLNALNPLLAIVGLEPRLCICDIYAGLS 518 L +D EAL ++ II L + + K++N ++PLL L+ RLCICD+ L+ Sbjct: 1276 VSLLLLLNDIRREALLAIQDIIAYLGMESTSKIINTVSPLLVDAELDVRLCICDLLESLA 1335 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,907,667 Number of Sequences: 27288 Number of extensions: 243280 Number of successful extensions: 548 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 534 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 546 length of database: 11,516,596 effective HSP length: 96 effective length of database: 8,896,948 effective search space used: 1147706292 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19821870|gb|BQ047894.1|BQ047894 952045F11.x2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (531 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g27930.1 expressed protein / ;supported by full-length cDN... 220 4e-58 >At3g27930.1 expressed protein / ;supported by full-length cDNA: Ceres:103959. Length = 425 Score = 220 bits (560), Expect = 4e-58 Identities = 102/169 (60%), Positives = 134/169 (78%), Gaps = 2/169 (1%) Frame = -2 Query: 530 HLVVQRRVKNPFEDDQVVGITNYIDFGLELAAKVDKDKV--SGDGNSFQLAASWQANKNF 357 H+VVQRRV+NPFE++QVVGITNYIDFG EL ++VD K + + Q+AASWQANKNF Sbjct: 256 HVVVQRRVQNPFEENQVVGITNYIDFGFELQSRVDDSKTPPNAPDSLLQVAASWQANKNF 315 Query: 356 LLKGKLGPSTSSVALAFKSWWRPSFTFSVTAVNDHSKGTTSYGFGLRVEDLRRASYQRAD 177 LLKGK+G +S+++LAFKSWW+PSF F+++A +H G GFGLRV++LR ASYQRAD Sbjct: 316 LLKGKVGAHSSTLSLAFKSWWKPSFAFNISATTNHRTGNVQCGFGLRVDNLREASYQRAD 375 Query: 176 PNYVMLTPSKEHLAPGVLREYGKRPMFQTQIDSGNYDHLPTELKPLGRI 30 PN+VMLTP+KEHLA G++ + GKRPM+Q +D+ N+ LP EL+P +I Sbjct: 376 PNFVMLTPNKEHLAEGIVWKMGKRPMYQADVDAENFSELPKELRPSQKI 424 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,098,610 Number of Sequences: 27288 Number of extensions: 259313 Number of successful extensions: 644 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 631 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 643 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 745241396 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19821832|gb|BQ047856.1|BQ047856 952045B09.x2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (621 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g08960.1 hypothetical protein / similar to hypothetical pr... 228 3e-60 >At3g08960.1 hypothetical protein / similar to hypothetical proteins: GB:S61997, [Saccharomyces cerevisiae], GB:CAA22859 [Schizosaccharomyces pombe] Length = 754 Score = 228 bits (580), Expect = 3e-60 Identities = 106/198 (53%), Positives = 139/198 (69%) Frame = +3 Query: 27 SLEQRKKNFAAVASDMLKVILSGDRVVLLCNILVRRYFIFTAKDLEEWSENPESFHHEQN 206 + EQRKKN + ++ +L +R+VLLCN+LVRRYF+ TA DLEEW +NPESFHHEQ+ Sbjct: 297 TFEQRKKNASNTVGGIVSSLLPNERIVLLCNVLVRRYFVLTASDLEEWYQNPESFHHEQD 356 Query: 207 LVQWTEKKRPCAEALFIVIFEKYREV*TFKFSLWL*VYSML*CSHDHVSLQLLAPVVVSV 386 ++QWTEK RPCAEAL++V+FE Y QLL P+VVS+ Sbjct: 357 MIQWTEKLRPCAEALYMVLFENYS--------------------------QLLGPIVVSI 390 Query: 387 LREAMAISPPQETEVTAGMLLKDASYTAAGHVYYELSNYLSFNEWLHGSLSIEISNHHPN 566 L+EAM PP TE+T +LLKDA+Y A +VYYELSNYL+F +W +G+LS+E+SN HPN Sbjct: 391 LQEAMNNCPPSVTEITPALLLKDAAYAATAYVYYELSNYLNFRDWFNGALSLELSNDHPN 450 Query: 567 MRIIRRKIALLLGHWISE 620 RII RK+A++LGHW+SE Sbjct: 451 RRIIHRKVAMILGHWVSE 468 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,648,969 Number of Sequences: 27288 Number of extensions: 248039 Number of successful extensions: 705 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 688 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 704 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 990590196 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19821810|gb|BQ047834.1|BQ047834 952016H01.x2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (441 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g68900.1 hypothetical protein / predicted by genscan 155 6e-39 >At1g68900.1 hypothetical protein / predicted by genscan Length = 656 Score = 155 bits (393), Expect = 6e-39 Identities = 79/146 (54%), Positives = 106/146 (72%) Frame = +3 Query: 3 DANQKWTYEQAVEFGSRVQSLHLEYIEEPVSSVYDLIKFCEKNVLPVALDETIDNLRGDV 182 DAN +WT+E+A EFG V S +L+YIEEPV + DLI+F E+ LPVALDET+D+ Sbjct: 151 DANCRWTFEEAREFGLLVNSCNLKYIEEPVQNKDDLIRFHEETGLPVALDETLDDFEECP 210 Query: 183 ICKLHQFVHPGIIALVIKPSVVGGFENAAHIAKWAQLHDKMAVISSTYESSIGLASYIQX 362 + L ++ HPGI+A+VIKPSVVGGFENAA IA+WAQ H KMAVIS+ YES +GL++YI Sbjct: 211 LRMLTKYTHPGIVAVVIKPSVVGGFENAALIARWAQQHGKMAVISAAYESGLGLSAYILF 270 Query: 363 AHYVDQQSSIVSRIRNKDTCRATAHG 440 A Y++ ++ S + + T + AHG Sbjct: 271 ASYLEMENVKASTEQKQGTPPSVAHG 296 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,947,269 Number of Sequences: 27288 Number of extensions: 167824 Number of successful extensions: 496 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 482 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 494 length of database: 11,516,596 effective HSP length: 90 effective length of database: 9,060,676 effective search space used: 507397856 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19770567|gb|BQ035288.1|BQ035288 952078H09.y2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (588 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g60640.1 hypothetical protein / predicted by genemark.hmm 70 6e-13 >At1g60640.1 hypothetical protein / predicted by genemark.hmm Length = 325 Score = 70.5 bits (171), Expect = 6e-13 Identities = 48/179 (26%), Positives = 82/179 (44%), Gaps = 35/179 (19%) Frame = +2 Query: 41 EDNSWNDVDGASIDDDEHSSPRDEERHEISLPIMKSSGANADSAKS--------ADNTDD 196 + +S +D S D D + ++ E S+ + G ++ +S D+ DD Sbjct: 13 DKDSGSDSSCFSSDSDPETEESEQSEEEDSVAEVSEDGDDSGDDESPAAGEDADVDDGDD 72 Query: 197 AVDAD--------------------------FPNCILKCKSVFKCKLCPRIMCLNVEMVK 298 DAD C+++ KSV+KC+ CP ++CLN ++ Sbjct: 73 NSDADDYGGTLEKMSMNRFLEEPPEEEEENYILGCMIQSKSVYKCRYCPTVVCLNENTMQ 132 Query: 299 IHLKSKRHARSEKLLGEGRLKLMLNSDGELE-EEQETHAERHARTIALSQQVQKQKKDS 472 H+ SK+HAR EKL+ EG+++ +LE QE + + R+ ++ QKQ+KDS Sbjct: 133 AHVSSKKHARMEKLVKEGKIRTDDEEVDDLETASQEKEKKGNRRSQRQGKRSQKQEKDS 191 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,419,062 Number of Sequences: 27288 Number of extensions: 188586 Number of successful extensions: 779 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 723 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 769 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 904103924 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19770516|gb|BQ035237.1|BQ035237 952078C09.y2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (620 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g22590.1 unknown protein / ; supported by cDNA: gi_17529301 252 1e-67 >At3g22590.1 unknown protein / ; supported by cDNA: gi_17529301 Length = 415 Score = 252 bits (644), Expect = 1e-67 Identities = 123/172 (71%), Positives = 148/172 (85%), Gaps = 1/172 (0%) Frame = +3 Query: 75 LGDGVVPIILVPSASQTLITIYNVKEFLEDGVFVPSEERMRATKGGKPESVTVQKKLIRT 254 +G+GV PIILVPSA QTLITIYNVKEFLEDGV++P++ + + KG KP+ +TVQKK R Sbjct: 248 IGEGV-PIILVPSAFQTLITIYNVKEFLEDGVYIPNDVKAKEMKGLKPDCITVQKKFSRD 306 Query: 255 -ERAGGAGGAVSFEVRDKPASLKSDDWGRVVAVFVLGKEWQFKDWPFKDHVEIFNRVIGF 431 ER ++EVRDKP++LK DDW RVVAVFVLGK+WQFKDWPFKDHVEIFN++IGF Sbjct: 307 RERV-----VTAYEVRDKPSALKPDDWDRVVAVFVLGKDWQFKDWPFKDHVEIFNKIIGF 361 Query: 432 YVRFEDDSVEAAKVVKQWNVKIISISKNKRHQDRTAALEVWERLEEFMRART 587 ++RFEDDS+E+AK VKQWNVKIISISKNKRHQDR AALEVWE+LEEF+R+R+ Sbjct: 362 FLRFEDDSIESAKTVKQWNVKIISISKNKRHQDRAAALEVWEKLEEFVRSRS 413 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,831,025 Number of Sequences: 27288 Number of extensions: 318345 Number of successful extensions: 1398 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 1230 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1369 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 990590196 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19770512|gb|BQ035233.1|BQ035233 952078C01.y2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (514 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g45150.1 hypothetical protein / predicted by genemark.hmm 198 2e-51 >At1g45150.1 hypothetical protein / predicted by genemark.hmm Length = 687 Score = 198 bits (503), Expect = 2e-51 Identities = 88/113 (77%), Positives = 102/113 (89%) Frame = -2 Query: 513 TGFLYWGSNCYEKAMIPSAEICFRRGLPPGDGVLFYPGEVFSSSHEPVASTRLERILSGM 334 TGFLYWG+NCYEKA +PSAE+ FRRGLPPGDGVL+YPGEVFSSS EPVAS RLER+LSG+ Sbjct: 573 TGFLYWGANCYEKATVPSAEVKFRRGLPPGDGVLYYPGEVFSSSSEPVASLRLERLLSGL 632 Query: 333 QDIEYLKLYSSRYGREEGLALIEKTGMYLGPDRYTLDHGPVDVMRGEVYRTCR 175 QD EYLKLY S+YGREE + L+EKTG+Y GP+RYTL+H P+DV+RGEVY TCR Sbjct: 633 QDYEYLKLYESKYGREEAMGLLEKTGVYTGPERYTLEHRPIDVLRGEVYNTCR 685 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,254,415 Number of Sequences: 27288 Number of extensions: 283462 Number of successful extensions: 701 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 683 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 701 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 702475800 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19770508|gb|BQ035229.1|BQ035229 952078B08.y2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (525 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g15352.1 Expressed protein / ; supported by cDNA: gi_14794... 83 1e-16 At1g53030.1 cytochrome C oxidase assembly protein, putative / ... 79 1e-15 >At3g15352.1 Expressed protein / ; supported by cDNA: gi_14794881_gb_AF349684.1_AF349684 Length = 74 Score = 82.8 bits (203), Expect = 1e-16 Identities = 34/38 (89%), Positives = 36/38 (94%) Frame = -1 Query: 294 ICCACPDTKRLRDECVVEHGEPACTKWIEAHKRCLRAE 181 ICCACPDTK+LRDEC+VEHGE ACTKWIEAHK CLRAE Sbjct: 33 ICCACPDTKKLRDECIVEHGESACTKWIEAHKICLRAE 70 >At1g53030.1 cytochrome C oxidase assembly protein, putative / similar to GB:5851903 from [Rattus norvegicus];supported by full-length cDNA: Ceres:11496. Length = 72 Score = 79.0 bits (193), Expect = 1e-15 Identities = 32/38 (84%), Positives = 35/38 (91%) Frame = -1 Query: 294 ICCACPDTKRLRDECVVEHGEPACTKWIEAHKRCLRAE 181 ICCACPDTK+LRDEC+VEHGE ACTKWIEAH CLR+E Sbjct: 31 ICCACPDTKKLRDECIVEHGESACTKWIEAHILCLRSE 68 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,703,127 Number of Sequences: 27288 Number of extensions: 162809 Number of successful extensions: 512 Number of sequences better than 1.0e-05: 4 Number of HSP's better than 0.0 without gapping: 500 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 512 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 727283772 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19770494|gb|BQ035215.1|BQ035215 952078A05.y2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (556 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g12530.1 unknown protein / 213 5e-56 >At3g12530.1 unknown protein / Length = 191 Score = 213 bits (542), Expect = 5e-56 Identities = 104/146 (71%), Positives = 120/146 (81%) Frame = +3 Query: 117 VAEDEIVEIVPNIRMDALNMICGDFGPFFPQIPTKVPLWLAVALKKRSKCTIRTPDWMTV 296 +AEDE+VEIVPN+ M+ LN I GDFG F PQIPTKVPLWLAVALK+R KCT R P WM+V Sbjct: 1 MAEDELVEIVPNMNMEQLNFISGDFGRFIPQIPTKVPLWLAVALKRRGKCTFRPPGWMSV 60 Query: 297 DRLTQVLEAERESPREFQPLPFHYIEISKLLFDHARDDISDAYLVRSLIEDIRDVRFHKV 476 D LTQ+LEAERES FQ LPF Y+EI++LLFDHARDDI D Y+VRSL+EDIRDVR HK+ Sbjct: 61 DNLTQILEAERESQSTFQALPFSYVEIARLLFDHARDDIPDMYMVRSLVEDIRDVRLHKL 120 Query: 477 ETGLETISGRTHAVKLKNLSAMEVNI 554 ET L + G T AVK+ N+SAMEVNI Sbjct: 121 ETNLGSFQG-TSAVKISNVSAMEVNI 145 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,329,152 Number of Sequences: 27288 Number of extensions: 235621 Number of successful extensions: 762 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 744 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 761 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 817071892 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19770448|gb|BQ035169.1|BQ035169 952077C05.y2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (669 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g04690.1 unknown protein / ; supported by cDNA: gi_19715625 228 3e-60 >At2g04690.1 unknown protein / ; supported by cDNA: gi_19715625 Length = 210 Score = 228 bits (580), Expect = 3e-60 Identities = 106/165 (64%), Positives = 133/165 (80%) Frame = +1 Query: 64 KPSPSEATATARWLAAQNTWGVLSTISSDLSGAPFGNVVSYSDGVPGEGRGIPYFYLTTL 243 KP + A+ARWL +QN+WGVLST+S D GAPFGNVVS+SDG+P +G GIPYFYLTTL Sbjct: 36 KPDRHDYAASARWLVSQNSWGVLSTLSVDHKGAPFGNVVSFSDGLPEKGNGIPYFYLTTL 95 Query: 244 DPSARDALEDERTSFTLSEFPLGTCGEIDPENPTCAKLTLNGKLKMVDLQSSEADLAKSA 423 DP+AR+AL+D+R S +SE PLGTC DP NPTC+KLTL GKL +++ S EA++AK A Sbjct: 96 DPTARNALKDQRASLAISESPLGTCTR-DPMNPTCSKLTLTGKLLILEGGSEEAEVAKKA 154 Query: 424 LFTKHPEMKDWPKNHHFKIFKMEIENIFLIDWFGGPKPISPSQYL 558 LFTKHPEM DWPK+H F+ FK+EI +IFLI+W+GG KPI+ +YL Sbjct: 155 LFTKHPEMMDWPKDHDFRFFKLEIIDIFLINWYGGAKPITVDEYL 199 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 15,486,965 Number of Sequences: 27288 Number of extensions: 365133 Number of successful extensions: 886 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 858 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 884 length of database: 11,516,596 effective HSP length: 96 effective length of database: 8,896,948 effective search space used: 1121015448 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19770364|gb|BQ035085.1|BQ035085 1091012C12.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (602 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g77550.1 hypothetical protein / similar to GB:CAB55886 [Ho... 191 2e-49 >At1g77550.1 hypothetical protein / similar to GB:CAB55886 [Homo sapiens], GB:BAA09774 [Homo sapiens], GB:CAA87778 [Caenorhabditis elegans] Length = 883 Score = 191 bits (485), Expect = 2e-49 Identities = 105/204 (51%), Positives = 133/204 (64%), Gaps = 4/204 (1%) Frame = +3 Query: 3 LNLWGNKLRDPEKVMQEIRKCTKLKALWLNENPVLGKS---IDKAVLDGLSGLEIYNSHF 173 L+L GNK+ + V+QEI K LKALWLN+NPVL KS + +L G LEIYNS F Sbjct: 186 LSLIGNKIESADVVIQEIVKFKNLKALWLNDNPVLQKSERQMADEILQGCPSLEIYNSCF 245 Query: 174 TSKAGEWALGFCADIVGADNPCSSVESTLFGSIGTIDLSDRCIHKLP-EVFXXXXXXXXX 350 T G WALGFC DI G DNP + ++ ++DLS+R IH L + F Sbjct: 246 TPNYGLWALGFCGDIFGKDNPGCVQQDQPLCNVTSLDLSNRSIHNLVNKAFSVHEMPLLS 305 Query: 351 XXNVRGNPLDQISGDDLLKLFGGFTQLQELEVDIPGPLGNSAISILESLPNLSLLNGVDS 530 N+RGN LDQ S +LL++ F L LEVDIPGPLG +A+ ILESL NLSLLNGVD+ Sbjct: 306 HLNIRGNSLDQNSVGELLEVLKLFPSLSSLEVDIPGPLGINALEILESLSNLSLLNGVDT 365 Query: 531 SSIIESGKHIVDSALEPRLPEWSP 602 + I+E+GKH+VDS L+PR+PE +P Sbjct: 366 AKILETGKHVVDSMLQPRIPELNP 389 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,067,799 Number of Sequences: 27288 Number of extensions: 304032 Number of successful extensions: 926 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 886 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 923 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 948861544 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19770263|gb|BQ034984.1|BQ034984 1091011C12.x1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (533 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g20835.1 hypothetical protein / 92 1e-19 >At2g20835.1 hypothetical protein / Length = 68 Score = 92.4 bits (228), Expect = 1e-19 Identities = 42/68 (61%), Positives = 52/68 (75%) Frame = -3 Query: 486 MVFFCFLVEQRRTVRSSKPAAGICSRCGGCASVADMETATRVCYLLTVHRRTWRAIICTF 307 M FCFLV+QR+ VR KPAAG+CSRCGG A VADM T+TR C + +R+ W+AI+C Sbjct: 1 MAIFCFLVDQRKKVRGRKPAAGLCSRCGGGAVVADMRTSTRFCG-VPFYRKAWKAIVCHI 59 Query: 306 CGAMLKSY 283 CGA+LKSY Sbjct: 60 CGAVLKSY 67 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,023,255 Number of Sequences: 27288 Number of extensions: 130775 Number of successful extensions: 528 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 501 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 527 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 754220208 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19770156|gb|BQ034877.1|BQ034877 1091010B08.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (580 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g45830.1 DNA-binding protein - like / DNA-binding protein... 264 3e-71 >At3g45830.1 DNA-binding protein - like / DNA-binding protein R kappa B, Homo sapiens, PIR:S52863 Length = 1298 Score = 264 bits (674), Expect = 3e-71 Identities = 126/187 (67%), Positives = 155/187 (82%) Frame = +1 Query: 10 KVQELKAQKSLNTISPSSDEARTYFQREEFLRYSIPDRAFCYTAADGEKSIVAPLRRGGG 189 + ++L+AQKSL+TI+ SS+EAR YF++EEFLRYSIPDRAF YTAADG+KSIVAPLRRGGG Sbjct: 1016 RFKDLRAQKSLSTITQSSEEARAYFRKEEFLRYSIPDRAFVYTAADGKKSIVAPLRRGGG 1075 Query: 190 KPTANARGHPMLLPDRPPHVTILCLVRDAASRLPARTGTRADVCTLLRDSQYLNHEEANK 369 KPT+ AR H ML +RPPHVTILCLVRDAA+RLP GTRADVCTL+RDSQY+ + ++ Sbjct: 1076 KPTSKARDHFMLKRERPPHVTILCLVRDAAARLPGSIGTRADVCTLIRDSQYIVEDVSDS 1135 Query: 370 EAAINQVVSGALDRLHYERDPCVLYDNDKKLWTYLHRGRXXXXXXXXGTSSTKKWKRPRR 549 + +NQVVSGALDRLHYERDPCV +D+++KLW YLHR R GTSSTKKWKRP++ Sbjct: 1136 Q--VNQVVSGALDRLHYERDPCVQFDSERKLWVYLHRDREQEDFEDDGTSSTKKWKRPKK 1193 Query: 550 DVSDAAE 570 + ++ E Sbjct: 1194 EAAEQTE 1200 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.316 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,684,280 Number of Sequences: 27288 Number of extensions: 237074 Number of successful extensions: 600 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 581 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 599 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 877249352 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19770131|gb|BQ034852.1|BQ034852 1091009H04.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (568 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g15200.1 expressed protein / EST gb|H77143 comes from this... 182 8e-47 >At1g15200.1 expressed protein / EST gb|H77143 comes from this gene; supported by cDNA: gi_15293206_gb_AY051037.1_ Length = 423 Score = 182 bits (463), Expect = 8e-47 Identities = 88/136 (64%), Positives = 112/136 (81%), Gaps = 2/136 (1%) Frame = +3 Query: 3 ARVAAKAEEKRLELLYIQWTEHHKRLSNFLRTKAEPPIYYMPTKPIIDDPAIVEQNKEKV 182 ARVAAKAE+K+LELL++QW+EH K+LSNF+RTKAEP IYY P KP+ +D + VEQ KE+ Sbjct: 231 ARVAAKAEQKKLELLFLQWSEHQKKLSNFIRTKAEPRIYYAPVKPLEEDTSEVEQQKERT 290 Query: 183 FEEWKSMRRAELTQFQKQVEEQYLSNVERQLERIQNARNARKANGPG-NMQE-MDKELDT 356 F EWK+ RR E++++QK++EEQ L NVE++LER QNAR ARKAN G N+QE MDKEL+T Sbjct: 291 FLEWKAARRQEVSEYQKEIEEQCLGNVEKELERWQNARKARKANNEGMNLQETMDKELET 350 Query: 357 HRAEHGPKPRRVPEEG 404 HR EHGPK R++P G Sbjct: 351 HRMEHGPKKRKIPGGG 366 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,663,325 Number of Sequences: 27288 Number of extensions: 200261 Number of successful extensions: 722 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 706 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 721 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 841443256 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19770114|gb|BQ034835.1|BQ034835 1091009F04.y1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (622 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g49970.1 putative protein / contains similarity to pyridox... 177 5e-45 >At5g49970.1 putative protein / contains similarity to pyridoxamine 5-phosphate oxidase;supported by full-length cDNA: Ceres:6244. Length = 466 Score = 177 bits (448), Expect = 5e-45 Identities = 77/119 (64%), Positives = 100/119 (83%) Frame = +1 Query: 1 WNEMNRQVRVEGSAEKVPEAESDKYFHSRPRGSQLGAIVSKQSTVIAGREVLQQDYKKLE 180 W +NRQVR+EG E++PE+ES+ YFHSRPRGSQ+GAIVSKQS+V+ GR VL +Y++L Sbjct: 348 WEILNRQVRIEGPVERIPESESENYFHSRPRGSQIGAIVSKQSSVVPGRHVLYDEYEELT 407 Query: 181 QKYSDGSLIPKPEYWGGYKLTPTLFEFWQGQQSRLHDRLQYSQREVDGSTVWHIERLSP 357 ++YSDGS+IPKP+ WGG++L P LFEFWQGQ SRLHDRLQYS ++V+G+ W I RL+P Sbjct: 408 KQYSDGSVIPKPKNWGGFRLKPNLFEFWQGQPSRLHDRLQYSLQDVNGNPAWKIHRLAP 466 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,333,754 Number of Sequences: 27288 Number of extensions: 315048 Number of successful extensions: 940 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 916 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 940 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 990590196 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19769779|gb|BQ034500.1|BQ034500 1091003A07.x2 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (573 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g23090.1 Expressed protein / ; supported by full-length cD... 135 2e-32 >At2g23090.1 Expressed protein / ; supported by full-length cDNA: Ceres: 14468. Length = 78 Score = 135 bits (339), Expect = 2e-32 Identities = 65/78 (83%), Positives = 68/78 (86%), Gaps = 1/78 (1%) Frame = -3 Query: 445 MGGGNGQKSKMARERNLEKNKGA-KGSQLETNKKAMSIQCKVCMQTFMCTTTEVKCREHA 269 MGGGN QKS MAR +NLEK K A KGSQLE NKKAMSIQCKVCMQTF+CTT+EVKCREHA Sbjct: 1 MGGGNAQKSAMARAKNLEKAKAAGKGSQLEANKKAMSIQCKVCMQTFICTTSEVKCREHA 60 Query: 268 EAKHPKTDVYQCFPHLKK 215 EAKHPK DV CFPHLKK Sbjct: 61 EAKHPKADVVACFPHLKK 78 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,619,154 Number of Sequences: 27288 Number of extensions: 264568 Number of successful extensions: 733 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 707 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 731 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 859346304 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19769502|gb|BQ034223.1|BQ034223 1091006G07.x1 1091 - Immature ear with common ESTs screened by Schmidt lab Zea mays cDNA, mRNA sequence (431 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g30820.1 Expressed protein / ; supported by full-length cD... 109 5e-25 >At4g30820.1 Expressed protein / ; supported by full-length cDNA: Ceres: 14193. Length = 178 Score = 109 bits (273), Expect = 5e-25 Identities = 52/87 (59%), Positives = 70/87 (79%) Frame = -2 Query: 430 LRRRIASIFNKTQEHFPTLKDYNDYLEEVEDMTFNLIEGIDVEEIEAKIARYQQENAEQI 251 +RRRI+ I+N+ +E FP+LKDYNDYLEEVE M F+LI+GI+VE IE KI RY +ENAEQI Sbjct: 16 VRRRISEIYNRREEEFPSLKDYNDYLEEVECMVFDLIDGINVEAIEQKIKRYSKENAEQI 75 Query: 250 YLSRAKRAEDLAAALKASRMNPVKAEA 170 ++RA++AE+L AAL A + P + +A Sbjct: 76 MINRARKAEELTAALAACKAKPPQTDA 102 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,779,324 Number of Sequences: 27288 Number of extensions: 169636 Number of successful extensions: 460 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 452 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 460 length of database: 11,516,596 effective HSP length: 90 effective length of database: 9,060,676 effective search space used: 480215828 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19437282|gb|BM953692.1|BM953692 952064H07.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (425 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g15140.1 expressed protein / ; supported by cDNA: gi_16930... 220 2e-58 >At3g15140.1 expressed protein / ; supported by cDNA: gi_16930516_gb_AF419612.1_AF419612 Length = 337 Score = 220 bits (560), Expect = 2e-58 Identities = 97/141 (68%), Positives = 116/141 (81%) Frame = +2 Query: 2 EQRTTEYIEGKYGKFGVDRVWHDTATPFKQVLQEFEDWLGNHNLWKKEQGGSLNRGAFVT 181 EQ +YIEGKYG+ GVDRVWHDTA PFKQV++EFE WL H+LW K+ LN AFVT Sbjct: 170 EQAINKYIEGKYGELGVDRVWHDTAIPFKQVVEEFEVWLAEHDLWDKDTDWGLNDAAFVT 229 Query: 182 CGNWDLKTKVPEQCKVSKINLPTYFMEWINLKDIYLNFYNRRATGMMTMMRELQLPIVGN 361 CGNWD+KTK+PEQC VS INLP YFMEWINLKD+YLNFY R A GM++MMR+ + ++G+ Sbjct: 230 CGNWDIKTKIPEQCVVSNINLPPYFMEWINLKDVYLNFYGREARGMVSMMRQCGIKLMGS 289 Query: 362 HHLGIDDSKNIARVVQRMLAD 424 HHLGIDD+KNI RVVQRML++ Sbjct: 290 HHLGIDDTKNITRVVQRMLSE 310 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,762,970 Number of Sequences: 27288 Number of extensions: 203981 Number of successful extensions: 601 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 584 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 600 length of database: 11,516,596 effective HSP length: 89 effective length of database: 9,087,964 effective search space used: 472574128 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19437058|gb|BM953468.1|BM953468 952062H09.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (487 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g29660.1 putative protein / predicted proteins, Arabidopsi... 128 2e-30 >At4g29660.1 putative protein / predicted proteins, Arabidopsis thaliana; supported by full-length cDNA: Ceres: 13465. Length = 103 Score = 128 bits (321), Expect = 2e-30 Identities = 53/83 (63%), Positives = 69/83 (82%) Frame = +3 Query: 27 YADRQFHKWEKTVLWDMVEPYRPPRSFAPLVGTYVAAFYTGVVGAAVTEQLYKEKYWEDH 206 YAD +++K+E+ +W+M+EPYR P++F L+ YVAAFYTGV+GAAVTEQLYKEK+WE+H Sbjct: 12 YADYKYNKFERFAVWEMIEPYRRPKTFTTLITIYVAAFYTGVIGAAVTEQLYKEKFWEEH 71 Query: 207 PGEAVPIMPPKFYWGPWRVMNGE 275 PG+ VP+M P FY GPWRV GE Sbjct: 72 PGKTVPLMKPVFYRGPWRVYRGE 94 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,494,443 Number of Sequences: 27288 Number of extensions: 190444 Number of successful extensions: 673 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 646 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 673 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 632337160 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19437030|gb|BM953440.1|BM953440 952062E02.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (385 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g53710.1 cell division control protein, putative / similar... 65 8e-12 >At1g53710.1 cell division control protein, putative / similar to SP:P40986 from [Saccharomyces cerevisiae] Length = 393 Score = 65.5 bits (158), Expect = 8e-12 Identities = 36/84 (42%), Positives = 53/84 (62%), Gaps = 3/84 (3%) Frame = +2 Query: 35 RGSVVARATAGRHRLEPDSSIRVDMGSEMTSEDGGK---LARAGKSGARKVLQRLFRVIQ 205 +G+ V R+ A ++ E I + M S++ + GG + A KS + V+QR+ R I Sbjct: 312 KGNAVMRSAARKNASE---QIELVMDSDVNASAGGSDPLMRSASKSRTKLVIQRVIRTIM 368 Query: 206 SVLVIAALNAPLYMMLLFKDWIDR 277 V+VIAALN P+YMMLLFKDWI++ Sbjct: 369 MVIVIAALNVPIYMMLLFKDWIEQ 392 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,367,247 Number of Sequences: 27288 Number of extensions: 150729 Number of successful extensions: 477 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 470 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 477 length of database: 11,516,596 effective HSP length: 87 effective length of database: 9,142,540 effective search space used: 365701600 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19436955|gb|BM953365.1|BM953365 952061C12.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (499 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g04900.1 expressed protein / ;supported by full-length cDN... 223 5e-59 >At1g04900.1 expressed protein / ;supported by full-length cDNA: Ceres:98874. Length = 442 Score = 223 bits (567), Expect = 5e-59 Identities = 106/158 (67%), Positives = 132/158 (83%) Frame = +2 Query: 26 ALFSSGILAGNKPILVRDFVRSALYDPNHGYFSKRAGPVGVLDASIRFNQLEGRSAYIQH 205 A FS+ L G++P+LVRDF+ +ALYDP GYFS+R+ VGVL+ SI+FNQLEGR AY++ Sbjct: 29 AFFSTQKLIGDEPVLVRDFIHTALYDPIQGYFSQRSKSVGVLERSIKFNQLEGRKAYMKL 88 Query: 206 LDKLYKKHDIAWFTPVELFKPWYAYAIAASILRTANLSVPLKIYEIGGGSGTCAKCILDY 385 L+K+YK+ DI+WFTPVELFKPWYA+ IA +ILRT NLSVPLKIYEIGGGSGTCAK +LDY Sbjct: 89 LEKVYKQSDISWFTPVELFKPWYAHGIAEAILRTTNLSVPLKIYEIGGGSGTCAKGVLDY 148 Query: 386 MMLNAPPKVYNDMKYISVEISSSLAEKQLETVGEVQSH 499 +MLNAP ++Y +M Y S+EIS SLA+ Q ETV +V SH Sbjct: 149 IMLNAPERIYKNMSYTSIEISPSLAKIQKETVAQVGSH 186 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,794,768 Number of Sequences: 27288 Number of extensions: 261324 Number of successful extensions: 803 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 793 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 803 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 657445300 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19436953|gb|BM953363.1|BM953363 952061C10.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (563 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g21820.1 putative protein / calmodulin-binding protein SHA... 103 6e-23 >At4g21820.1 putative protein / calmodulin-binding protein SHA1, Mus musculus,AF062378 Length = 1088 Score = 103 bits (257), Expect = 6e-23 Identities = 65/156 (41%), Positives = 90/156 (57%), Gaps = 15/156 (9%) Frame = -3 Query: 561 LVAAGAVDILLKQIYLLXRGIADQEVLMQVFLTLRNIARYSNLRQVLASTAESVEIIFHE 382 LVAAGA++ LL I R I DQ+V TL ++ARY + L +T S++ IF E Sbjct: 933 LVAAGAIEKLLTLIRSASRSIPDQQVSKHALSTLGHLARYPQMADELINTKGSIQTIFWE 992 Query: 381 LLR-------------NKADGFFIASDILKSLCESKEGRETVQALSHLIKRLRNLVHDLE 241 LLR NK + +FIASD+LK +C S +G E V+ L L+KRL LV +L Sbjct: 993 LLRSSFSSQVFFLSFKNKEEAYFIASDVLKKICSSHKGVEAVRKLPALVKRLHVLVEELT 1052 Query: 240 KKVELDKRN--GRTGAMKATNLRRLREAATLYHILT 139 +K ++KRN G+TG K+ RRL+EA L ++T Sbjct: 1053 RKANIEKRNVKGQTGKEKSE--RRLKEAIELVKLIT 1086 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,382,760 Number of Sequences: 27288 Number of extensions: 232133 Number of successful extensions: 665 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 638 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 664 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 832491732 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19436444|gb|BM952854.1|BM952854 952059F11.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (593 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g14240.1 putative protein / various predicted proteins fro... 175 1e-44 >At5g14240.1 putative protein / various predicted proteins from D. melanogaster, H. sapiens and S. pombe;supported by full-length cDNA: Ceres:37589. Length = 256 Score = 175 bits (444), Expect = 1e-44 Identities = 83/121 (68%), Positives = 103/121 (84%), Gaps = 1/121 (0%) Frame = +1 Query: 88 GIPECGLLQNCLEELATRYPATKFVKIISTDCIPNYPDRNVPTILVYNNSAVKGTYVGLQ 267 G+ EC LL CL+EL +RYPATKFVKIISTDCIPNYPD N+PT+LVY++ A+KGT+VGL+ Sbjct: 131 GVAECSLLLGCLDELGSRYPATKFVKIISTDCIPNYPDCNLPTLLVYHHGAIKGTHVGLK 190 Query: 268 KFGGKRCTPESVALALCQSDPVLNDGHGG-SDSSRDNVIEGVRRKFIEKVVAQHEEREEE 444 F G+RCTPESVAL LCQS+PVLNDG GG DSSR+ V+ GVRR+FIE+VV HE+++ + Sbjct: 191 SF-GRRCTPESVALLLCQSEPVLNDGKGGDDDSSREAVMAGVRRQFIERVVKDHEDKDND 249 Query: 445 D 447 D Sbjct: 250 D 250 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,422,431 Number of Sequences: 27288 Number of extensions: 340978 Number of successful extensions: 1088 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 1050 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1086 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 922006972 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19436394|gb|BM952804.1|BM952804 952059C06.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (625 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g34090.1 hypothetical protein / predicted by genefinder, s... 196 6e-55 >At2g34090.1 hypothetical protein / predicted by genefinder, similar to GP|1301832|gnl|PID|e239862|Z71287 Length = 488 Score = 196 bits (497), Expect(2) = 6e-55 Identities = 94/120 (78%), Positives = 107/120 (88%) Frame = +2 Query: 74 VVEELKKVTTRVAHVLPVSDDGGSTAEIVRVLGGPAVGDIRSRCLRLSDESTAEALSVRK 253 VVEELKK+TTRVAHVLPVSDDGGSTAEIVRVLGGPAVGDIRSRCLRLSDEST+EAL+VR+ Sbjct: 66 VVEELKKLTTRVAHVLPVSDDGGSTAEIVRVLGGPAVGDIRSRCLRLSDESTSEALAVRR 125 Query: 254 LLGHRLPIDPSEAKLEWYQIVEGDHTLWEGVSRPYRETIRAFLVYFHSEILRRSAEMFCF 433 LLGHRLPID +AK +WY IVEG+H+LW+GVSRPY ETIRAFL+YF +E++ E F Sbjct: 126 LLGHRLPIDAHQAKKDWYDIVEGNHSLWDGVSRPYSETIRAFLIYFQNEVMLTDTEKHVF 185 Score = 35.8 bits (81), Expect(2) = 6e-55 Identities = 15/22 (68%), Positives = 19/22 (86%) Frame = +3 Query: 30 PPSLLVFSGGTAFNGLLKN*RK 95 PPSLLVFSGGT FNG+++ +K Sbjct: 51 PPSLLVFSGGTGFNGVVEELKK 72 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,475,639 Number of Sequences: 27288 Number of extensions: 288747 Number of successful extensions: 838 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 805 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 834 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 999514432 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19436199|gb|BM952609.1|BM952609 952076D03.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (594 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g31780.1 unknown protein / 282 8e-77 >At1g31780.1 unknown protein / Length = 706 Score = 282 bits (722), Expect = 8e-77 Identities = 148/223 (66%), Positives = 163/223 (72%), Gaps = 26/223 (11%) Frame = +2 Query: 2 HHQRAGLELMDMMSVYQEGAYERLCRWV--------------------------QAECKK 103 HHQRAGLELMDMM+VYQEGAYERLCR + AEC+K Sbjct: 175 HHQRAGLELMDMMAVYQEGAYERLCRTIICFQKGSPFLNSFRANEKTQYWLLKQNAECRK 234 Query: 104 LGDTDNPEVSELLKMAVCCLKERPVLFKYCAEEVANMRHHALFRRFISALTRGGPGGFPR 283 LGDTDNPEVSELL+ AV CLKERPVLFKYCAEEV N+RH+ALFRRFISALTRGGPGG PR Sbjct: 235 LGDTDNPEVSELLRTAVRCLKERPVLFKYCAEEVGNLRHNALFRRFISALTRGGPGGMPR 294 Query: 284 PIEVHAHDPLRYVGEMLGWLHQALASERELIVVLLDPDAMTDSGPTFRRQSGRDGDSSKG 463 PIEVHAHDPLRYVG+MLGWLHQALASEREL+ L D D+ + + + G Sbjct: 295 PIEVHAHDPLRYVGDMLGWLHQALASERELVHALFDIDSADHQSNA--KNTSENIALKAG 352 Query: 464 EPDVTSVLDRIFEGACRPFKGRVEQGLQAQPSLIGSFKLSNTL 592 E D T VLDRIFEG CRPFK RVEQ LQ+QPSLI S+KL+NTL Sbjct: 353 ESDFTFVLDRIFEGVCRPFKVRVEQVLQSQPSLIISYKLTNTL 395 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,035,968 Number of Sequences: 27288 Number of extensions: 271289 Number of successful extensions: 759 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 734 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 756 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 922006972 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19436192|gb|BM952602.1|BM952602 952076C06.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (637 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g25830.1 expressed protein / ; supported by cDNA: gi_15810... 288 1e-78 >At2g25830.1 expressed protein / ; supported by cDNA: gi_15810382_gb_AY056230.1_ Length = 331 Score = 288 bits (738), Expect = 1e-78 Identities = 144/211 (68%), Positives = 178/211 (84%), Gaps = 1/211 (0%) Frame = +2 Query: 2 RSCKIAGRKDAQNLKKMKRNSKIGKEIVAAIKKGGPNPSSNTTLSAILEKARELDVPREI 181 RS KIAGRK AQ+ KK K +IGKE+V+A+KKGGPNP SNTTL+ IL+KA+ELDVP++I Sbjct: 87 RSSKIAGRKGAQDSKKAKLYCRIGKEVVSAVKKGGPNPVSNTTLATILDKAKELDVPKDI 146 Query: 182 LERNIKRASEKGQDTYTEKVYEVYGFGGVGMVVEVLTDKITRSVADIRNVVKDCGAKLAD 361 +ERNIKRASEKGQ+ + EK+YEVYG+GGV MVVEVLTDKI RSVA IR+VVKD G K+AD Sbjct: 147 VERNIKRASEKGQEAFIEKIYEVYGYGGVSMVVEVLTDKINRSVAAIRSVVKDYGGKMAD 206 Query: 362 PGSVTFRFRQARVVNIKVTDADKDQLLTVALDAGADDVLE-PNFDDDDSEEEAERFYKIV 538 GSV F+F++ RVVNIKVT+ADKDQLL +ALDAGA+DV+E P ++DD E+ ER+YKIV Sbjct: 207 SGSVMFKFKRVRVVNIKVTEADKDQLLIIALDAGAEDVIEPPTYEDDTDEDREERYYKIV 266 Query: 539 TTSENYPGLLSKLQEEGLKVETDNGYELGPL 631 T++ENY +LSKL++EG+ E DNG EL PL Sbjct: 267 TSNENYSTILSKLRDEGVNFEPDNGSELLPL 297 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,796,860 Number of Sequences: 27288 Number of extensions: 252490 Number of successful extensions: 1062 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 982 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1052 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 1035211376 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19436134|gb|BM952544.1|BM952544 952075E04.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (653 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g30790.1 putative protein / 84 5e-17 >At4g30790.1 putative protein / Length = 1148 Score = 84.3 bits (207), Expect = 5e-17 Identities = 47/119 (39%), Positives = 69/119 (57%), Gaps = 1/119 (0%) Frame = -1 Query: 362 KLIVHVAENGHTLEFQCGGDTLVEAIQHSIQLHCEIPPADQLLLCGNISLDGANALATYK 183 KL++ VAENGH+ EFQC T VE++ ++ I +DQLLL ++ L+ L+ + Sbjct: 14 KLLLCVAENGHSFEFQCSETTSVESVMRFVESVSGIALSDQLLLSLDMKLEPQKLLSAFG 73 Query: 182 LPRDDREVFLYNKARLLADSRPPAPESLYIXXXXXXXXXXXXXXXPS-DASADPALKAL 9 LP DREVF++NKA L ++S PP+PE + + D ++DPALKAL Sbjct: 74 LPASDREVFVFNKAMLQSNSHPPSPEDVDLQDVADALPPASLHEHHPLDDASDPALKAL 132 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,967,805 Number of Sequences: 27288 Number of extensions: 197841 Number of successful extensions: 694 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 674 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 694 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 1088756792 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19351633|gb|BM896165.1|BM896165 952067D07.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (515 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g67140.1 hypothetical protein / predicted by genemark.hmm 145 9e-36 >At1g67140.1 hypothetical protein / predicted by genemark.hmm Length = 2149 Score = 145 bits (367), Expect = 9e-36 Identities = 84/164 (51%), Positives = 104/164 (63%), Gaps = 38/164 (23%) Frame = -2 Query: 508 VAYVSQVQGTLFLAMEILLLEENGYVDLRQGIGHLINAIVAVIGPELAPDSTFFSRCKSV 329 +++VS VQ L LA++ILL EE+G++DL QGIG LINAIVAV+GPEL+P S FSRCKSV Sbjct: 831 LSFVSHVQAALGLALDILLTEESGWIDLSQGIGRLINAIVAVLGPELSPGSILFSRCKSV 890 Query: 328 IAEISSSNEMATLLE--------------------------------------SVRFSQQ 263 IAEISS E+ TLLE SV F+QQ Sbjct: 891 IAEISSWQEIPTLLEYVNAFLFATIILIHCYYYMNYLYPVSSLIFIVNLNIFRSVCFTQQ 950 Query: 262 LVLFAPQAVPVHLHVQGLIPMLYSRQPSLRYLAVSTLHHLIERD 131 L+LFAPQAV VH+HV+ L+ L SRQP +R L+VSTL HL+E+D Sbjct: 951 LILFAPQAVSVHIHVKNLLMTLASRQPIIRRLSVSTLRHLVEKD 994 Score = 64.7 bits (156), Expect = 3e-11 Identities = 29/45 (64%), Positives = 36/45 (79%) Frame = +1 Query: 1 PAAMIDENIEENLFSMLDEETDSETAMLVRATIIRLLYTSCPVCP 135 P ++IDE IE+NLF MLDEETDSE L+R+T+IRLLY +CP P Sbjct: 995 PVSVIDEQIEDNLFQMLDEETDSEIGNLIRSTLIRLLYATCPSRP 1039 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,435,707 Number of Sequences: 27288 Number of extensions: 167438 Number of successful extensions: 505 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 498 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 505 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 711481900 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19351626|gb|BM896158.1|BM896158 952067C10.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (611 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g13540.1 putative cap-binding protein / ; supported by cD... 218 3e-57 >At2g13540.1 putative cap-binding protein / ; supported by cDNA: gi_15192737_gb_AF272891.1_AF272891 Length = 848 Score = 218 bits (554), Expect = 3e-57 Identities = 113/203 (55%), Positives = 142/203 (69%), Gaps = 2/203 (0%) Frame = +2 Query: 8 ANLQDALHNENRDRIRILLRFLSGLMCSKVVVPSSIIETFETLLSSAVTILDDETGNPSW 187 AN Q AL + N + IRILLRF++ L+CSKV+ P+S+I FETLLSSA T +D+E GNPSW Sbjct: 88 ANFQVALDSGNCNSIRILLRFMTSLLCSKVIQPASLIVVFETLLSSAATTVDEEKGNPSW 147 Query: 188 QPRADFYVYCILASLPWAGPELFEQVPDEFERVLFGIQSYISIRRHFDDIAFSVFETDEG 367 QP+ADFYV CIL+SLPW G EL EQVPDE ERVL GIQ+Y+SIR++ + F E Sbjct: 148 QPQADFYVICILSSLPWGGSELAEQVPDEIERVLVGIQAYLSIRKNSSTSGLNFFHNGEF 207 Query: 368 HSP-NKKDFMEDLWERIQALSRNGWKVKSVPKPHLPFEAQLVAGKSHRLX-XXXXXXXXX 541 S +KDF+EDL +RIQ+L+ NGWK++SVP+PHL FEAQLVAGK H L Sbjct: 208 ESSLAEKDFVEDLLDRIQSLASNGWKLESVPRPHLSFEAQLVAGKFHELRPIKCMEQPSP 267 Query: 542 XXXXXEMLKGQEKHEADMKYPQR 610 G++KH+A +YPQR Sbjct: 268 PSDHSRAYSGKQKHDALTRYPQR 290 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,947,904 Number of Sequences: 27288 Number of extensions: 265508 Number of successful extensions: 755 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 745 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 753 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 963817488 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19351533|gb|BM896065.1|BM896065 952072G04.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (500 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g03910.1 hypothetical protein / predicted by genscan 241 1e-64 >At1g03910.1 hypothetical protein / predicted by genscan Length = 672 Score = 241 bits (616), Expect = 1e-64 Identities = 106/113 (93%), Positives = 109/113 (95%) Frame = +3 Query: 3 PRKPKYFNRVHTGYEWNKYNQTHYDHDNPPPKIVQGYKFNIFYPDLVDKSKAPTYSIEKD 182 PRKPKYFNRVHTGYEWNKYNQTHYDHDNPPPKIVQGYKFNIFYPDLVDK KAP Y+IEKD Sbjct: 554 PRKPKYFNRVHTGYEWNKYNQTHYDHDNPPPKIVQGYKFNIFYPDLVDKIKAPIYTIEKD 613 Query: 183 GSAGETCFIRFHAGPPYEDIAFRIVNKEWEYSHKKGFKCTFERGILHLYFNFK 341 G++ ETC IRFHAGPPYEDIAFRIVNKEWEYSHKKGFKCTFERGILHLYFNFK Sbjct: 614 GTSAETCMIRFHAGPPYEDIAFRIVNKEWEYSHKKGFKCTFERGILHLYFNFK 666 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,401,160 Number of Sequences: 27288 Number of extensions: 245076 Number of successful extensions: 725 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 692 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 725 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 666451400 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19351510|gb|BM896042.1|BM896042 952072D10.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (494 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g37190.1 tubulin-like protein / 160 2e-40 >At4g37190.1 tubulin-like protein / Length = 562 Score = 160 bits (406), Expect = 2e-40 Identities = 83/161 (51%), Positives = 111/161 (68%), Gaps = 2/161 (1%) Frame = +2 Query: 2 FFVEECDHIQGIQFLVDDSGGFASVAAQFLESIVDDYTNTPVMLYCVRNPDSYGSSRNQR 181 FFVEECDHIQGI+FLVDDSGGF++VAA FLE++ D+YTN PV+LY VR P S SS + Sbjct: 203 FFVEECDHIQGIKFLVDDSGGFSAVAADFLENMADEYTNVPVLLYSVRTPMSQMSS---K 259 Query: 182 ETIIRSLHDAVSLSKLSYYCNLMVPIGXXXXXXXXXXXXIK--DEKHFHSSAICAAAMHS 355 +T+ LHDA+S S+LS +C L PIG + DEK + SSA+ AAA+HS Sbjct: 260 KTVSNKLHDAISFSRLSSFCKLFTPIGLPSLTGSKASKFLNLGDEKPYRSSAVYAAALHS 319 Query: 356 LSIPFRLQHVGPASDSAHSSGKLDIGELVHILSDQGRQNMI 478 +IPFR+Q +SDS+ S +D+ LV +L+ +GRQN++ Sbjct: 320 STIPFRMQPT--SSDSSEVSNSMDVNTLVQLLTGRGRQNIV 358 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,297,459 Number of Sequences: 27288 Number of extensions: 233985 Number of successful extensions: 638 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 630 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 635 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 648439200 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19351489|gb|BM896021.1|BM896021 952072B05.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (529 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g19140.1 expressed protein / ;supported by full-length cD... 154 3e-38 >At4g19140.1 expressed protein / ;supported by full-length cDNA: Ceres:151921. Length = 303 Score = 154 bits (388), Expect = 3e-38 Identities = 81/171 (47%), Positives = 109/171 (63%), Gaps = 3/171 (1%) Frame = +2 Query: 8 STCRILSSGVDLRSSKVCELGLLNYRAKHVFYPSSKRRFRCHDDYYWASVLQVEYTEYFS 187 S C+I+SS VDLRSSKVC +GLLN +A+HVFYP + +FRC DYYWASV +VEY + Sbjct: 69 SRCKIVSSSVDLRSSKVCGIGLLNVKAQHVFYPFERDKFRCRYDYYWASVFKVEYKDNLM 128 Query: 188 GQLSYAVAEAPKEALPHNCRPDFGAAWSTTLKFKVNESYSCRYMLGSNKADIHSNKLFNC 367 GQ A +EAP EALP CRP+FGAA T FKVNE+Y C Y LG K ++ + F C Sbjct: 129 GQTRLAFSEAPNEALPPECRPNFGAALLTKDNFKVNETYDCWYTLGIPKIKLYRDSFFGC 188 Query: 368 TAEEPSTGELFKRILILFSEMYVS--EDFSSGRMLVY-VAAGVALGMLSSM 511 A++ S ++ K+ I+ S++ S S+ + Y V AG+ G +S+ Sbjct: 189 QADDLSFTDIAKQYSIVISKLLQSWLNGESTPKYWKYDVIAGIVSGFATSI 239 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,627,180 Number of Sequences: 27288 Number of extensions: 204277 Number of successful extensions: 535 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 528 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 535 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 736262584 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19351230|gb|BM895762.1|BM895762 952065B06.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (615 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g11790.1 expressed protein / supported by full-length cDNA... 128 2e-30 >At4g11790.1 expressed protein / supported by full-length cDNA: Ceres:151415. Length = 443 Score = 128 bits (322), Expect = 2e-30 Identities = 69/136 (50%), Positives = 87/136 (63%), Gaps = 1/136 (0%) Frame = -3 Query: 613 SPSXXXXXXXXXXXXXETKCKVYVKHDD-ATKGWKDIGVGQLSIRSKEGTEKASKESTPT 437 SPS E KCK+YVK D A KGWKD G G L I+ KEG +K +KES PT Sbjct: 312 SPSVKKTEEKGITVVHEVKCKLYVKSSDPADKGWKDKGTGNLYIKCKEGVDKGTKESKPT 371 Query: 436 IVIRNDVGKILLNALIYKGIKMNLQKNTVASIFHTSDAQSSESAGDTVVARTYLFRLKNV 257 I++RNDVGK+LLNAL+Y G+K + QKN + +IFH+SD + + V RT+L R KN Sbjct: 372 ILVRNDVGKLLLNALLYAGMKTSPQKNALVAIFHSSD-----DSNENVTPRTFLIRTKNA 426 Query: 256 EEATKLSTAIKENAPS 209 E L+TAI+E APS Sbjct: 427 EARDNLATAIQEYAPS 442 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,925,366 Number of Sequences: 27288 Number of extensions: 242530 Number of successful extensions: 614 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 597 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 612 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 972741724 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19351133|gb|BM895665.1|BM895665 952076G04.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (562 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g00450.1 hypothetical protein / 143 5e-35 >At4g00450.1 hypothetical protein / Length = 2124 Score = 143 bits (361), Expect = 5e-35 Identities = 78/125 (62%), Positives = 94/125 (74%), Gaps = 20/125 (16%) Frame = -3 Query: 335 ALKKRLRAINESRAQKRK--------------------AGQVYGVPLSGSLLIKPGVYPE 216 A++K LRAINESRA KRK AGQVYGVPLSGSLL KPG +PE Sbjct: 12 AVRKCLRAINESRALKRKVIKRLSDSNPFQFIFCAEFRAGQVYGVPLSGSLLCKPG-FPE 70 Query: 215 QRPCNEDARRKWIEALAQPNKRLRSLSEHVPHGYRRKSLFEGLIRYNVPLLRATWFIKVT 36 QR C E+ +++WIE+L+Q +KRLRSL++++P GYRRK+LFE LIR NVPLLRATWFIKVT Sbjct: 71 QRSCGEETKKRWIESLSQQHKRLRSLADNIP-GYRRKTLFEVLIRNNVPLLRATWFIKVT 129 Query: 35 YLNQL 21 YLNQ+ Sbjct: 130 YLNQV 134 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,897,344 Number of Sequences: 27288 Number of extensions: 238477 Number of successful extensions: 686 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 672 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 683 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 835029516 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19351055|gb|BM895587.1|BM895587 952075F03.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (498 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g18700.1 unknown protein / ; supported by cDNA: gi_17979006 108 2e-24 >At1g18700.1 unknown protein / ; supported by cDNA: gi_17979006 Length = 700 Score = 108 bits (269), Expect = 2e-24 Identities = 63/171 (36%), Positives = 97/171 (55%), Gaps = 7/171 (4%) Frame = +3 Query: 6 LSKAATAFKDDRLTFVWLDGEVQKQICAFYLATDYT-GACGPRDFGDDNDKPEIFIVRFQ 182 ++ AA+A K RL+F WLDGE Q + C FY+ ++ + CG R D P I IVR+ Sbjct: 445 ITPAASAHKSKRLSFAWLDGEAQNKYCFFYVQSETSYDTCGTRRA--PIDVPRILIVRYH 502 Query: 183 RNATYEALXAEKKNNLVDVLQGQHAD----ASQLVARYKGPVEIEEINRWISQIIKDGDT 350 RNAT A +K + + AD A+QLV Y G EI +I +W+S+++ DGD Sbjct: 503 RNATETANAEQKSSKWPKTVWQSEADDVDPAAQLVVSYDGTAEIPQIIKWLSKMVDDGDN 562 Query: 351 REIPYFTSKVPDLVPE--ETSKAWSSKSIRSAGKSLKENIGFHFQDYLTHP 497 R +P++ +K P+LVPE E ++ K++++ K L ++ +DYL P Sbjct: 563 RNLPFYRAKTPELVPESAEPMRSGVPKTVKATQKLL--SLWNRIKDYLADP 611 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,770,972 Number of Sequences: 27288 Number of extensions: 220351 Number of successful extensions: 652 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 642 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 650 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 657445300 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19351030|gb|BM895562.1|BM895562 952075C05.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (474 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g16570.1 unknown protein / contains similarity to beta-1,4... 143 5e-35 >At1g16570.1 unknown protein / contains similarity to beta-1,4 mannosyltransferase GI:6970470 from [Homo sapiens] Length = 465 Score = 143 bits (360), Expect = 5e-35 Identities = 75/124 (60%), Positives = 92/124 (73%), Gaps = 1/124 (0%) Frame = -2 Query: 377 ERRRASVVVLGDIGRSPRMQYHSLSLANQASMEVDIVANGGSDPHLSLRENPSIHIHEMK 198 +R RA VVVLGD+GRSPRMQYH+LSLA QAS +VDIVA GGS PH ++ +PSIHIH M Sbjct: 3 KRGRACVVVLGDLGRSPRMQYHALSLARQASFQVDIVAYGGSIPHEAVLNHPSIHIHTMA 62 Query: 197 TVQ-LTGISKISGALSLLLKAAVQFILLVWFLCFKIPRPDVFFVQNPPSVPTLAAVKLAA 21 + + KI ++LLLKA +QF +L+WFL K+P PD+F VQNPPSVPTL AVK A+ Sbjct: 63 QPRFIQYFPKILYPVTLLLKAFIQFTMLLWFLFVKVPAPDIFLVQNPPSVPTLIAVKWAS 122 Query: 20 G*ER 9 R Sbjct: 123 SWRR 126 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,100,671 Number of Sequences: 27288 Number of extensions: 217847 Number of successful extensions: 678 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 664 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 676 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 596203608 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19351029|gb|BM895561.1|BM895561 952075C03.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (568 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g18070.1 N-acetylglucosamine-phosphate mutase / ; support... 156 8e-39 >At5g18070.1 N-acetylglucosamine-phosphate mutase / ; supported by cDNA: gi_166925_gb_L11367.1_ATHUNIDA Length = 277 Score = 156 bits (394), Expect = 8e-39 Identities = 78/117 (66%), Positives = 97/117 (82%) Frame = +2 Query: 218 VRCASLDGDADRLVYFRLSSASDNRVDLVDGDKILSLFALFIREQLDIVNNNGGQANKSL 397 +RCASLDGDADRLVYF + S S +V+L+DGDKILSLFALFI+EQL+ + ++ + Sbjct: 1 MRCASLDGDADRLVYFYIPSDSSEKVELLDGDKILSLFALFIKEQLNALEDD----EERK 56 Query: 398 SARLGIVQTAYANGASTLFLKSLGLEVVFTPTGVKYLHKRALEYDIGIYFEANGHGT 568 +RLG+VQTAYANGAST +LK LGL+VVF TGVK+LH++A E+DIGIYFEANGHGT Sbjct: 57 QSRLGVVQTAYANGASTDYLKHLGLDVVFAKTGVKHLHEKAAEFDIGIYFEANGHGT 113 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,041,906 Number of Sequences: 27288 Number of extensions: 285671 Number of successful extensions: 899 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 877 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 898 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 841443256 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19351014|gb|BM895546.1|BM895546 952074H12.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (597 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g64960.1 hypothetical protein / predicted by genscan+ 128 3e-30 >At1g64960.1 hypothetical protein / predicted by genscan+ Length = 1227 Score = 128 bits (321), Expect = 3e-30 Identities = 69/187 (36%), Positives = 112/187 (58%) Frame = +1 Query: 1 KLLIPSYFPSKLSPKEACARCIALIKRSPTAGARFCEFALSEGSSPRSIVELVKYSITLA 180 +LLIPSYFPS+ +EAC RC LI R+P AGARFCEF +S G++ +S++ LV + + Sbjct: 533 RLLIPSYFPSRKRAEEACQRCRTLINRNPKAGARFCEFLVSLGATVKSVLHLVGFFLNSV 592 Query: 181 LSQTGLNSDQIDGLIIASVNLIKSLSNERSSLAALREFLANGKLRLVLQVAVSERARAAL 360 LS L +Q +GL+ A+ L K L + +A+L+E L KL+ +L A + +A++++ Sbjct: 593 LSGDKLLENQTEGLLRAAYYLCKDLVADSGCMASLKELLPGEKLKSLLAFAPTAQAQSSV 652 Query: 361 LGIVPVVLPDELSVLHEECMDIVVNAARISKQEEYQETVLEAHKLIVLGGWSDELFEALT 540 + I+ +V PD +S + E+CM++VVN + Q + HKL++ +L T Sbjct: 653 IDIITMVSPDIVSEVLEDCMNLVVNCGGLPSDAGRQTELRSVHKLLLSSNAFCDLIGTFT 712 Query: 541 NTLQSKA 561 + +Q A Sbjct: 713 SIMQKTA 719 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.317 0.133 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,159,854 Number of Sequences: 27288 Number of extensions: 243034 Number of successful extensions: 629 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 609 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 628 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 930958496 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19351001|gb|BM895533.1|BM895533 952074H04.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (568 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g61150.1 putative protein / Atu, Drosophila melanogaster, ... 132 1e-31 >At5g61150.1 putative protein / Atu, Drosophila melanogaster, EMBL:DMU75467 Length = 633 Score = 132 bits (332), Expect = 1e-31 Identities = 81/185 (43%), Positives = 113/185 (60%), Gaps = 14/185 (7%) Frame = -1 Query: 517 DQRMESDAKGLDSE--DEGYQQRTVSSRRRGVVASESEGSEDNYYADGAQEDEEPRQTRK 344 D + ES+A+ DS+ +E + R RR+ VV S SE S + +Y EDEE QTR Sbjct: 100 DPQEESEARDSDSDNKEEEHGGRVAKKRRQEVVESGSERSGEKHYES---EDEEVDQTRS 156 Query: 343 QSSP------MXXXXXXXXXXXVFGDSDEDEPAPYRSRHEIDEDSHRSPMEDEDQHEKDM 182 SP + VFG SD+++ Y R+++++D HRSP+EDE+ EKD+ Sbjct: 157 PRSPSEEKEEVQVAQSDVNIRNVFGSSDDEDAEEY-VRNDVEQDEHRSPIEDEEGSEKDL 215 Query: 181 QPDDTVAD-----EDMRYESD-ENRELKPKEKPVGPPLDLVVPFKQPPARPDKMNVIKVS 20 +PDD V D ED +YES+ E+ E + +E+PVGPPL++ VPF+ PP P KMN+IKVS Sbjct: 216 RPDDMVLDDIIPEEDPQYESEAEHVEARYRERPVGPPLEVEVPFRPPPGDPVKMNMIKVS 275 Query: 19 NIMGI 5 NIMGI Sbjct: 276 NIMGI 280 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,313,129 Number of Sequences: 27288 Number of extensions: 231583 Number of successful extensions: 2153 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 1364 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1711 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 841443256 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19350967|gb|BM895499.1|BM895499 952074F09.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (602 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g29420.1 hypothetical protein / 148 2e-36 >At4g29420.1 hypothetical protein / Length = 446 Score = 148 bits (373), Expect = 2e-36 Identities = 71/117 (60%), Positives = 92/117 (77%) Frame = +2 Query: 113 LVKLCLKNAWLSVDRLKKMPNLTYLTLEFIRLDDEDLNQLNECFPCLHTLNLIGVGGLKD 292 LVKL +KNAWLSV L +MPNL YLTLEFIRLDDE+L ++N+CFP L LNLIGVGGLK+ Sbjct: 153 LVKLEVKNAWLSVVGLTEMPNLRYLTLEFIRLDDENLEKVNDCFPFLQELNLIGVGGLKE 212 Query: 293 PKIHLLQLKACCWEVSNVPRSLVVHAPDLVFLELKCIRPDTLILDTPSLSTLKLTID 463 P+IH L LK+C W VSN P SL + AP+L+ L+LKC +P +L+++TP L L+++ Sbjct: 213 PRIHFLHLKSCHWTVSNAPLSLAIVAPNLLELKLKCNKPKSLLVETPKLVQCHLSVE 269 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,146,156 Number of Sequences: 27288 Number of extensions: 279369 Number of successful extensions: 794 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 770 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 794 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 948861544 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19350966|gb|BM895498.1|BM895498 952074F08.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (567 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g21150.1 putative XAP-5 protein [Homo sapiens] / 206 9e-54 >At2g21150.1 putative XAP-5 protein [Homo sapiens] / Length = 383 Score = 206 bits (523), Expect = 9e-54 Identities = 111/174 (63%), Positives = 119/174 (67%), Gaps = 21/174 (12%) Frame = +3 Query: 108 VKSDPRLSFCDDIENGSDEDDFENQETKKNGP--AKLGKDPTVETSFLPDXXXXXXXXXX 281 +K RLSF +D ENGSDEDD EN+ + KLGKDP+VET+FLPD Sbjct: 116 IKGSSRLSFAEDFENGSDEDDGENKSSGTGNLRCGKLGKDPSVETNFLPDSEREAEEQAE 175 Query: 282 XXXLKKQWSHEQELIK-----NEPLSITYSYWDGTGHRRAIQ--------------VRKG 404 LKKQW EQE IK NEPL ITYSYWDGTGHRR IQ VRKG Sbjct: 176 RERLKKQWLREQEQIKSCPFSNEPLEITYSYWDGTGHRRVIQASTNYTFVILLEPYVRKG 235 Query: 405 DTIGEFLRAVQQQLAPEFREVRTTSVENLLYVKEDLIIPHQHSFYELIINKARG 566 D IG FLRAVQQQLAP+FRE+RT SVENLLYVKEDLIIPHQHSFYELIINKARG Sbjct: 236 DPIGNFLRAVQQQLAPDFREIRTASVENLLYVKEDLIIPHQHSFYELIINKARG 289 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,206,323 Number of Sequences: 27288 Number of extensions: 157560 Number of successful extensions: 535 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 518 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 529 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 841443256 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19350961|gb|BM895493.1|BM895493 952074F06.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (572 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g05680.1 unknown protein / 172 1e-43 >At3g05680.1 unknown protein / Length = 2070 Score = 172 bits (436), Expect = 1e-43 Identities = 87/168 (51%), Positives = 116/168 (68%) Frame = +3 Query: 69 FCRSGLSFLLSQPEATELILLSLQDAEKMNKSECITLRQAFVLLSKGFFCRPKEVGLITE 248 F R+GLSFLL PE T I+ SL+ + +NK EC+ L A +L+SKGF C E+G+ E Sbjct: 531 FSRTGLSFLLHHPELTATIIQSLKGSVDLNKEECVPLHYASILISKGFTCSLLEIGINLE 590 Query: 249 LHLKVGSAATRILSVPPNSDELLWVLWELCAISRSDSGRQALLALGYFPEAISVLLRSLS 428 +HL+V SA R+L ++E LW+LWEL +SRSD GR+ALL LG FPEA++VL+ +L Sbjct: 591 MHLRVVSAVDRLLKSIQQTEEFLWILWELRDVSRSDCGREALLTLGVFPEALAVLIEALH 650 Query: 429 SYKDLDSVMAENGGSPLGLAIFHSAAEILEALAADSTASSLESWIGFA 572 S KD++ + +G SPL LAI HSAAEI E + +DSTAS L +WI A Sbjct: 651 SAKDMEPAVENSGISPLNLAICHSAAEIFEVIVSDSTASCLHAWIEHA 698 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,878,344 Number of Sequences: 27288 Number of extensions: 272377 Number of successful extensions: 785 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 765 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 785 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 859346304 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19350952|gb|BM895484.1|BM895484 952074E10.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (591 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g64790.1 hypothetical protein / predicted by genemark.hmm 318 1e-87 >At1g64790.1 hypothetical protein / predicted by genemark.hmm Length = 2428 Score = 318 bits (815), Expect = 1e-87 Identities = 159/193 (82%), Positives = 177/193 (91%) Frame = -3 Query: 589 QCLPKIVPKLAELLTDTHPKVQASGQTALQEVGSVIKNPEISALVPILLSALMDPNAHTK 410 QCLP++VPKL E+LTDTHPKVQ++GQ ALQ+VGSVIKNPEIS+LVP LL AL DPN +T+ Sbjct: 1295 QCLPRVVPKLTEVLTDTHPKVQSAGQLALQQVGSVIKNPEISSLVPTLLLALTDPNEYTR 1354 Query: 409 HSLDILLQTTFINSIDAPSLALLVPIVHRGLRERGVETKKKAAQIVGNMSSLVTEPKDMI 230 H+LD LLQTTF+NS+DAPSLALLVPIVHRGLRER ETKKKA+QIVGNM SLVTEPKDMI Sbjct: 1355 HALDTLLQTTFVNSVDAPSLALLVPIVHRGLRERSSETKKKASQIVGNMCSLVTEPKDMI 1414 Query: 229 PYISLLLPEVKKVLVDPIPEVRAVAARALGSLISGMGEEIFPDLVPWLLDTLKSDNSNVE 50 PYI LLLPEVKKVLVDPIPEVR+VAARA+GSLI GMGE+ FPDLVPWL +TLKSD SNVE Sbjct: 1415 PYIGLLLPEVKKVLVDPIPEVRSVAARAVGSLIRGMGEDNFPDLVPWLFETLKSDTSNVE 1474 Query: 49 RSGAAQGLSEVLA 11 R GAAQGLSEV+A Sbjct: 1475 RYGAAQGLSEVIA 1487 Score = 50.8 bits (120), Expect = 5e-07 Identities = 40/174 (22%), Positives = 82/174 (46%), Gaps = 6/174 (3%) Frame = -3 Query: 514 QTALQEVGSVIKNPE--ISALVPILLSALMD----PNAHTKHSLDILLQTTFINSIDAPS 353 Q AL +++ N + ++PIL+S L+ P++ + L + + Sbjct: 1662 QAALHVWKTIVANTPKTLKEIMPILMSTLISSLASPSSERRQVAGRSLGE-LVRKLGERV 1720 Query: 352 LALLVPIVHRGLRERGVETKKKAAQIVGNMSSLVTEPKDMIPYISLLLPEVKKVLVDPIP 173 L L++PI+ +GL++ V+ K++ I N ++ ++ L+P ++ L D Sbjct: 1721 LPLIIPILSKGLKDPDVD-KRQGVCIGLNEVMASAGRSQLLSFMDQLIPTIRTALCDSAL 1779 Query: 172 EVRAVAARALGSLISGMGEEIFPDLVPWLLDTLKSDNSNVERSGAAQGLSEVLA 11 EVR A A +L G + +++P LL+ L+ D + + A GL ++++ Sbjct: 1780 EVRESAGLAFSTLYKSAGLQAMDEIIPTLLEALEDDEMS---TTALDGLKQIIS 1830 Score = 49.3 bits (116), Expect = 1e-06 Identities = 38/176 (21%), Positives = 85/176 (47%), Gaps = 5/176 (2%) Frame = -3 Query: 574 IVPKLAELLTDTHPKVQASG-----QTALQEVGSVIKNPEISALVPILLSALMDPNAHTK 410 ++ L E L D + + G + +++G + + P + ++P+LL + D + Sbjct: 1179 LIVTLQEALIDRNSAKRREGALLAFECLCEKLGKLFE-PYVIKMLPLLLVSFSDQVGAVR 1237 Query: 409 HSLDILLQTTFINSIDAPSLALLVPIVHRGLRERGVETKKKAAQIVGNMSSLVTEPKDMI 230 + + + ++ + A + L++P + +GL ++ TK+ + Q++G M+ P+ + Sbjct: 1238 EAAECAARA-MMSQLSAYGVKLVLPSLLKGLEDKAWRTKQSSVQLLGAMAFCA--PQQLS 1294 Query: 229 PYISLLLPEVKKVLVDPIPEVRAVAARALGSLISGMGEEIFPDLVPWLLDTLKSDN 62 + ++P++ +VL D P+V++ AL + S + LVP LL L N Sbjct: 1295 QCLPRVVPKLTEVLTDTHPKVQSAGQLALQQVGSVIKNPEISSLVPTLLLALTDPN 1350 Score = 47.4 bits (111), Expect = 6e-06 Identities = 27/80 (33%), Positives = 44/80 (54%) Frame = -3 Query: 244 PKDMIPYISLLLPEVKKVLVDPIPEVRAVAARALGSLISGMGEEIFPDLVPWLLDTLKSD 65 PK + + +L+ + L P E R VA R+LG L+ +GE + P ++P L LK Sbjct: 1676 PKTLKEIMPILMSTLISSLASPSSERRQVAGRSLGELVRKLGERVLPLIIPILSKGLKDP 1735 Query: 64 NSNVERSGAAQGLSEVLADS 5 + + +R G GL+EV+A + Sbjct: 1736 DVD-KRQGVCIGLNEVMASA 1754 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,504,771 Number of Sequences: 27288 Number of extensions: 222139 Number of successful extensions: 769 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 728 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 764 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 913055448 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19350941|gb|BM895473.1|BM895473 952074E05.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (601 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g24350.1 putative protein / similar to unknown protein (re... 153 6e-38 >At5g24350.1 putative protein / similar to unknown protein (ref|NP_056993.1) Length = 2376 Score = 153 bits (387), Expect = 6e-38 Identities = 88/201 (43%), Positives = 118/201 (57%), Gaps = 1/201 (0%) Frame = +2 Query: 2 RMVITCQAGAYLHFEEIIDVAKLLGLRSXXXXXXXXXXXXXXXXXNGDLQLALDICLNLT 181 +M IT AYLH EE+I+VAKLLGL S GD+QLA D+CL LT Sbjct: 1076 KMAITGDPEAYLHGEELIEVAKLLGLNSSEDISSVKEAIAREAAIAGDMQLAFDLCLVLT 1135 Query: 182 KKSHGAVWDLCAAIARGPPLDNLDTGTREKLLGFALSHCDDDSVGELLNAWKELHAQGTF 361 K+ HG +WDL AAIAR P L+++D +R++LLGFAL HCDD+S+ ELL+AWK+ QG Sbjct: 1136 KEGHGPIWDLGAAIARSPALEHMDISSRKQLLGFALGHCDDESISELLHAWKDFDLQGQC 1195 Query: 362 EKLMITTATNPPNF-LIDGSSITPLPVQSVQDILDLRDDNGHDRRSDLVGIVKDMLSKVC 538 E L + + +N P F +DG V + D + D D++ DL KD +S V Sbjct: 1196 ETLGMLSESNSPEFQKMDG-------VSCLTDFPQMLDGLSSDQQLDL-DRAKDSISCVA 1247 Query: 539 LDFSNGDTHNWESMLEENRKL 601 D D+ + ES+L+EN KL Sbjct: 1248 KDMPVDDSVDLESLLKENGKL 1268 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,040,684 Number of Sequences: 27288 Number of extensions: 271681 Number of successful extensions: 720 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 701 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 720 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 939910020 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19350746|gb|BM895278.1|BM895278 952073B09.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (504 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g11560.1 putative protein / predicted proteins, Homo sapie... 270 4e-73 >At5g11560.1 putative protein / predicted proteins, Homo sapiens, Drosophila melanogaster, Caenorhabditis elegans Length = 955 Score = 270 bits (689), Expect = 4e-73 Identities = 132/164 (80%), Positives = 152/164 (92%) Frame = +1 Query: 13 DNKDVMKLILGKHNLSAPITSYARPEVAVKSQSYFFTHSVKAMAVTQTAKGITSKQLLIG 192 +NK+V KLILGKHNL+APITSY+RPEV KSQSYFF SVK +AVT TAKGITSKQLLIG Sbjct: 767 ENKNVWKLILGKHNLTAPITSYSRPEVFTKSQSYFFAQSVKTIAVTSTAKGITSKQLLIG 826 Query: 193 TIGDQVLALDKRYLDPRRSVNPTQQEKEEGIIPLTDSLPIIPQSFVTHSHQVEALRGIVS 372 TIGDQ+LALDKR++DPRR++NP+Q EKEEGIIPLTD+LPIIPQ++VTHSH+VE LRGIV+ Sbjct: 827 TIGDQILALDKRFVDPRRTLNPSQAEKEEGIIPLTDTLPIIPQAYVTHSHKVEGLRGIVT 886 Query: 373 IPAKLESTTLVFTYGVDLFYTQLAPSRTYDSLTDEFSYALLLIT 504 P+KLESTT VF YGVDLFYT+LAPS+TYDSLTD+FSYALLLIT Sbjct: 887 APSKLESTTHVFAYGVDLFYTRLAPSKTYDSLTDDFSYALLLIT 930 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,338,540 Number of Sequences: 27288 Number of extensions: 248251 Number of successful extensions: 700 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 684 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 700 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 675457500 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19350666|gb|BM895198.1|BM895198 952070A07.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (529 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g15566.1 hypothetical protein / 97 5e-21 >At4g15566.1 hypothetical protein / Length = 249 Score = 97.1 bits (240), Expect = 5e-21 Identities = 59/125 (47%), Positives = 77/125 (61%), Gaps = 3/125 (2%) Frame = +2 Query: 161 RFQKIVLSWDYLCLVAES--KGGKQAKV-LQHVKNTYVSVAEYLGVFEPLLFEEVKAQII 331 RF I+L WDY L E+ K K +K L VKNTY V +Y FEPLLFEEVKAQI+ Sbjct: 18 RFYNIILGWDYKQLTKENERKNRKDSKEKLNVVKNTYKDVDDYFETFEPLLFEEVKAQIL 77 Query: 332 QGRSNDEEESGMDWRRGAVGSCTESEGFHKLSVAVEDNFQDNVSENDLLLISKEKIEEGS 511 Q + D EE+ + R V C E EGFH L V E + +++NDLLL+SKE+++ S Sbjct: 78 QNK--DGEEASVCKMR-LVMECNEGEGFHFLLVTYEHEEDEYLAQNDLLLLSKEEVKGNS 134 Query: 512 TPNAY 526 P++Y Sbjct: 135 FPSSY 139 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,444,985 Number of Sequences: 27288 Number of extensions: 250211 Number of successful extensions: 901 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 859 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 900 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 736262584 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19350653|gb|BM895185.1|BM895185 952066H04.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (560 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g17560.1 putative protein / ;supported by full-length cDN... 124 3e-29 >At5g17560.1 putative protein / ;supported by full-length cDNA: Ceres:9667. Length = 177 Score = 124 bits (311), Expect = 3e-29 Identities = 59/79 (74%), Positives = 68/79 (85%) Frame = +3 Query: 267 DSLATKALEAKIKEQLEADTVTVVDTSGDGRHVCIDVISKAFEGKSAVNRQRMVYKAIWE 446 D +++E KIKEQL A++VTV D SGDGRHVCI+V+S AFEG+SAVNRQRMVYKAIWE Sbjct: 98 DQTLMQSMELKIKEQLNAESVTVTDMSGDGRHVCINVVSSAFEGQSAVNRQRMVYKAIWE 157 Query: 447 ELQSTVHAVDQMTTKTPDE 503 ELQ+ VHAVDQMTTKTP E Sbjct: 158 ELQNVVHAVDQMTTKTPSE 176 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,051,867 Number of Sequences: 27288 Number of extensions: 129574 Number of successful extensions: 360 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 357 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 360 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 835029516 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19272538|gb|BM888794.1|BM888794 952069E07.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (499 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g40480.1 nuclear pore protein -like / nuclear pore protein... 118 1e-27 >At5g40480.1 nuclear pore protein -like / nuclear pore protein gp210 precursor, rat, PIR:S04921 Length = 1919 Score = 118 bits (296), Expect = 1e-27 Identities = 59/151 (39%), Positives = 87/151 (57%) Frame = +3 Query: 27 GVLSRVVYRVEALKRLSFSDKVTIVLPATGQTEEVEVSYDTGEEAESPSSWGLTTTAVML 206 G+ Y+V L+ F+D++ I LPATGQ E++V YDTGE + S G + +L Sbjct: 1772 GIAGHAQYKVNVLRSEQFTDRIIITLPATGQIVEIDVCYDTGESLVASSKDGYSVLLKIL 1831 Query: 207 TCIIVTIVTVALFIKLLQRPIRQAPSGSXXXXXXXXXXXXXXXXXXXXXXXXNGQFSPRT 386 ++V +V+V + +K++ R Q P+G+ + + SPRT Sbjct: 1832 WGVLVLVVSVIILMKVIDR---QVPTGATGTATYSGNAAQGTPERRSGTVIYHEE-SPRT 1887 Query: 387 PQPFMEYVRRTIDDTPYYKRDARRRFNPQNT 479 P PFMEYV+RT+D+TPYY+R+ RRRFNPQNT Sbjct: 1888 PSPFMEYVKRTVDETPYYRREGRRRFNPQNT 1918 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,742,413 Number of Sequences: 27288 Number of extensions: 147421 Number of successful extensions: 436 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 420 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 434 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 657445300 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19272453|gb|BM888709.1|BM888709 952068B02.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (493 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g10370.1 putative glycerol-3-phosphate dehydrogenase / sim... 180 9e-70 >At3g10370.1 putative glycerol-3-phosphate dehydrogenase / similar to glycerol-3-phosphate dehydrogenase GB:BAA08926 [Mus musculus]; supported by cDNA: gi_17380777 Length = 629 Score = 180 bits (457), Expect(2) = 9e-70 Identities = 84/96 (87%), Positives = 89/96 (92%) Frame = +2 Query: 206 KEFETFAKVVVNAAGPFCDSVRKMADSTVAPMICPSSGVHIVLPDYYSPEGMGLIVPKTK 385 +EF ++AKVVVNAAGPFCDS+RKM D PMICPSSGVHIVLPDYYSPEGMGLIVPKTK Sbjct: 281 QEFNSYAKVVVNAAGPFCDSIRKMIDEDTKPMICPSSGVHIVLPDYYSPEGMGLIVPKTK 340 Query: 386 DGRVVFMLPWLGRTVAGTTDSNTAITMLPEPHEDEI 493 DGRVVFMLPWLGRTVAGTTDSNT+IT LPEPHEDEI Sbjct: 341 DGRVVFMLPWLGRTVAGTTDSNTSITSLPEPHEDEI 376 Score = 100 bits (248), Expect(2) = 9e-70 Identities = 48/62 (77%), Positives = 58/62 (93%) Frame = +1 Query: 13 LKGTVVYYDGQMNDSRLNVGLACTAAVVGAAVLNYAEVVSLIKDESGERIIGARVRDTLS 192 L+GTVVYYDGQMNDSRLNVGLACTAA+ GAAVLN+AEVVSLI D++ +RIIGAR+R+ L+ Sbjct: 220 LRGTVVYYDGQMNDSRLNVGLACTAALAGAAVLNHAEVVSLITDDATKRIIGARIRNNLT 279 Query: 193 GR 198 G+ Sbjct: 280 GQ 281 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,952,331 Number of Sequences: 27288 Number of extensions: 195213 Number of successful extensions: 456 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 448 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 456 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 639433100 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058676|gb|BM737343.1|BM737343 952055C09.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (602 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g12050.1 fumarylacetoacetate hydrolase-like protein / simi... 341 1e-94 >At1g12050.1 fumarylacetoacetate hydrolase-like protein / similar to fumarylacetoacetate hydrolase, gb|L41670 from Emericella nidulans; supported by cDNA: gi_20334819 Length = 421 Score = 341 bits (875), Expect = 1e-94 Identities = 152/200 (76%), Positives = 177/200 (88%) Frame = +1 Query: 1 RGPQTPVNPNWFYLPIGYNGRASSIVVSGTDVIRPRGQGHPTGNSAPYFGPSQKLDFELE 180 RGP+ +NPNWF LPI Y+GRASSIV+SGTD+IRPRGQGHP GNS PYFGPS+KLDFELE Sbjct: 147 RGPENAINPNWFRLPIAYHGRASSIVISGTDIIRPRGQGHPQGNSEPYFGPSKKLDFELE 206 Query: 181 MAAIVGPGNELGKPIDINDAEEHIFGLTLMNDWSARDIQAWETIPLGPFLGKSFSTTISP 360 MAA+VGPGNELGKPID+N+A +HIFGL LMNDWSARDIQAWE +PLGPFLGKSF TTISP Sbjct: 207 MAAVVGPGNELGKPIDVNNAADHIFGLLLMNDWSARDIQAWEYVPLGPFLGKSFGTTISP 266 Query: 361 WIVTLDALKPFMCEAPKQEPEPLPYLAEKNHINYDIPLEVLIKPKDQNVALIXTKTNFKH 540 WIVTLDAL+PF C+APKQ+P PLPYLAEK +NYDI LEV +KP ++ + + TK+NF++ Sbjct: 267 WIVTLDALEPFGCQAPKQDPPPLPYLAEKESVNYDISLEVQLKPSGRDDSCVITKSNFQN 326 Query: 541 LYWTATQQLTHHTINGCNMR 600 LYWT TQQL HHT+NGCN+R Sbjct: 327 LYWTITQQLAHHTVNGCNLR 346 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,517,376 Number of Sequences: 27288 Number of extensions: 258372 Number of successful extensions: 733 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 643 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 733 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 948861544 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058554|gb|BM737221.1|BM737221 952053F12.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (589 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g49930.1 putative protein / strong similarity to unknown p... 167 7e-43 >At5g49930.1 putative protein / strong similarity to unknown protein (gb|AAF56406.1) Length = 1080 Score = 167 bits (424), Expect(2) = 7e-43 Identities = 83/138 (60%), Positives = 110/138 (79%) Frame = +1 Query: 175 LAYGPALAEHIILDAGLVPSTKVGKDPESTINDSTVQSLMESITRFEDWLVDIISGQRIP 354 L YGP L+EHIILDAGLVP+TK+ +D + ++D+ +Q L++++ FEDWL DII+GQ++P Sbjct: 227 LGYGPQLSEHIILDAGLVPTTKLSEDKK--LDDNEIQLLVQAVIVFEDWLEDIINGQKVP 284 Query: 355 EGYILMQNKMTAKNITPLEEASINHKIYDEYCPVLLNQFKSREYNEFATFDAALDEFYSK 534 EGYILMQ ++ A + T E+ K+YDE+C +LLNQFKSR Y +F TFDAALDEFYSK Sbjct: 285 EGYILMQKQILANDTT--SESGGVKKMYDEFCSILLNQFKSRVYEKFETFDAALDEFYSK 342 Query: 535 IESQKVNQQQKAKEESAA 588 IESQ+ QQQKAKE+SA+ Sbjct: 343 IESQRSEQQQKAKEDSAS 360 Score = 23.5 bits (49), Expect(2) = 7e-43 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = +3 Query: 114 KVNNGAQSNKATLKTILGEAPGLWP 188 K N+ + + TLK ILG+A G P Sbjct: 207 KSNDSTGAKQYTLKNILGDALGYGP 231 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,997,207 Number of Sequences: 27288 Number of extensions: 219643 Number of successful extensions: 595 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 582 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 593 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 904103924 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058552|gb|BM737219.1|BM737219 952053F10.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (543 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g02220.1 unknown protein / 119 1e-44 >At3g02220.1 unknown protein / Length = 227 Score = 119 bits (297), Expect(2) = 1e-44 Identities = 54/131 (41%), Positives = 72/131 (54%) Frame = +1 Query: 127 RCRDQIDWKRRYGKYKPIVEPAKCQKCNKRNVRQAYHNVCRDCSKDLGICAKCCTCVNEL 306 RCR+QI WKR+YGKYK + E KCQKC KRNVRQAYH +C C+K+ +CAKCC V+++ Sbjct: 45 RCREQIAWKRKYGKYKTLTEATKCQKCTKRNVRQAYHKLCPGCAKEQKVCAKCCQSVDQI 104 Query: 307 VGRDANXXXXXXXXXXXXXXXXXXXXXXXXXXIMNKGKGGESGPSVPKIADRSREGDIFP 486 +GRD MNK +D S+ GD+FP Sbjct: 105 LGRDIYEVEAEQKLLDETIKNARERDRRTLLRAMNKDNKPNKSDEEASRSDSSKVGDVFP 164 Query: 487 AASLDEYAEQT 519 + SL+EYA ++ Sbjct: 165 STSLEEYANKS 175 Score = 78.2 bits (191), Expect(2) = 1e-44 Identities = 33/41 (80%), Positives = 37/41 (89%) Frame = +3 Query: 3 RQGPPKHQNRYAWKPNLGLKINETEPGGRFRPLSEITGVCH 125 RQGPPKHQN++AW P G+KINETE GGRFRPLSEITGVC+ Sbjct: 4 RQGPPKHQNKFAWVPKAGVKINETEVGGRFRPLSEITGVCY 44 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,693,419 Number of Sequences: 27288 Number of extensions: 251608 Number of successful extensions: 903 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 874 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 901 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 781156644 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058488|gb|BM737155.1|BM737155 952053B02.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (534 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g04950.1 unknown protein / 108 2e-24 >At3g04950.1 unknown protein / Length = 152 Score = 108 bits (269), Expect = 2e-24 Identities = 53/109 (48%), Positives = 73/109 (66%), Gaps = 7/109 (6%) Frame = +2 Query: 227 DKIKSTITGKKPEEASEAS----SFTLIH---TMEKARKLGTFKNFVAGRASEATVVNVF 385 D IK TG K E++ +FTL+ ++ AR+LG FK ++ GR+SEAT + F Sbjct: 2 DSIKGVFTGNKDTPLEESNLPVEAFTLLRFADELKNARRLGKFKQYIVGRSSEATFADAF 61 Query: 386 EKHSAVLRYLGAIDPTGENLRNTDKINATKHCNCTMADVEHILAKYTWA 532 EK AV+RYLGA+D TGENL+ + K +A KHC CT+ DVE+ L+K+TWA Sbjct: 62 EKQEAVIRYLGALDATGENLQASQKQDAAKHCKCTITDVENTLSKFTWA 110 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,212,479 Number of Sequences: 27288 Number of extensions: 174582 Number of successful extensions: 673 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 659 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 672 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 754220208 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058409|gb|BM737076.1|BM737076 952049B01.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (614 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g03250.1 expressed protein / EST gb|N96383 comes from this... 188 2e-48 >At1g03250.1 expressed protein / EST gb|N96383 comes from this gene;supported by full-length cDNA: Ceres:40461. Length = 245 Score = 188 bits (477), Expect = 2e-48 Identities = 92/176 (52%), Positives = 126/176 (71%) Frame = +3 Query: 69 MASADLLRREEFYSSLFDSAKGDGARSRSQLIERKIEALEDMATRVSNRRSRKWFNDRLL 248 MA+ +++RR+E S L D +GD + SR +E+KIEALE +A +VSNRRSR+W NDR+L Sbjct: 1 MAATNVMRRDE--SLLIDPQRGDTSVSRGLSLEKKIEALESLAGQVSNRRSRRWLNDRIL 58 Query: 249 IELVPRLYVEEIKGLFAPPPWGEQVPLSSFYRANVGEWDAFRSIDMDAEARLMQHMKKSL 428 +ELVPRL +EI+GLFAPPPWG+ VP S+F NVGEWD FR+IDMD EA +M + +S Sbjct: 59 MELVPRLDAQEIRGLFAPPPWGDDVPPSAFSLTNVGEWDKFRNIDMDKEANIMDSLNRSS 118 Query: 429 EKPRNHVDEGESIALNAWHRIDRHTREAIKRNFLPDLLGIYEERVRAFIEDASDKD 596 + + VD + LNAW RID TR+A++R+FLP+L+ YE + FIE+ + D Sbjct: 119 VRQKGSVDGDKIAVLNAWRRIDCRTRDALRRSFLPELIEGYESCISHFIEEGGEGD 174 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,829,840 Number of Sequences: 27288 Number of extensions: 268505 Number of successful extensions: 799 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 756 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 799 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 972741724 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058377|gb|BM737044.1|BM737044 952052F11.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (603 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g37460.1 putative protein / SPINDLY protein, Petunia x hyb... 277 3e-75 At4g12400.1 stress-induced protein sti1 -like protein / stres... 56 2e-08 At3g04240.1 putative O-linked GlcNAc transferase / similar to... 54 8e-08 At1g12270.1 hypothetical protein / ; supported by cDNA: gi_17... 53 1e-07 At1g62740.1 TPR-repeat protein / contains multiple TPR domain... 52 2e-07 At2g42810.1 putative phosphoprotein phosphatase / ; supporte... 51 5e-07 At4g22670.1 HSP associated protein like / p48, Homo sapiens, ... 50 9e-07 At3g17870.1 HSC70-interacting protein, putative / similar to ... 49 2e-06 >At4g37460.1 putative protein / SPINDLY protein, Petunia x hybrida, Y17720 Length = 1013 Score = 277 bits (708), Expect = 3e-75 Identities = 128/199 (64%), Positives = 167/199 (83%) Frame = -3 Query: 601 IGRGTAYAFQRELDSAISDFTKAIQSSPSAGEAWKRRGQARAALGEFKEAIEDLTKALEF 422 IGRGTAYAFQREL+SAI+DFTKAIQS+P+A EAWKRRGQARAALGE+ EA+EDLTKAL F Sbjct: 335 IGRGTAYAFQRELESAIADFTKAIQSNPAASEAWKRRGQARAALGEYVEAVEDLTKALVF 394 Query: 421 EPNSPDILHERGIVNFKFKDYNSALEDLSTCVKHDKKNSSAHTYLGLTLSALGEYKRAED 242 EPNSPD+LHERGIVNFK KD+ +A++DLS C+K +K N SA+TYLGL ++LGEYK+AE+ Sbjct: 395 EPNSPDVLHERGIVNFKSKDFTAAVKDLSICLKQEKDNKSAYTYLGLAFASLGEYKKAEE 454 Query: 241 EHVIGLKYDESFLDCWAHLAQLYLDLAYPEKLLNCLEKAIQIDSRFAKAYHLRGILYHGM 62 H+ ++ D ++L+ W HLAQ Y +LA K L C+E+ +Q+D+R KAYHLRG+++HG+ Sbjct: 455 AHLKSIQLDSNYLEAWLHLAQFYQELADHCKALECIEQVLQVDNRVWKAYHLRGLVFHGL 514 Query: 61 GRHRSAIKELSVALTYESS 5 G HR AI+ELS+ L+ E++ Sbjct: 515 GEHRKAIQELSIGLSIENT 533 >At4g12400.1 stress-induced protein sti1 -like protein / stress-induced protein sti1 -Glycine max,PID:g872116 Length = 558 Score = 55.8 bits (133), Expect = 2e-08 Identities = 25/96 (26%), Positives = 49/96 (51%) Frame = -3 Query: 595 RGTAYAFQRELDSAISDFTKAIQSSPSAGEAWKRRGQARAALGEFKEAIEDLTKALEFEP 416 +G + +++ A+ +++AI+ +P+ A+ R LG E ++D K +E +P Sbjct: 375 KGNGFFKEQKYPEAVKHYSEAIKRNPNDVRAYSNRAACYTKLGALPEGLKDAEKCIELDP 434 Query: 415 NSPDILHERGIVNFKFKDYNSALEDLSTCVKHDKKN 308 + +G + F K+Y+ A+E +KHD KN Sbjct: 435 SFTKGYSRKGAIQFFMKEYDKAMETYQEGLKHDPKN 470 >At3g04240.1 putative O-linked GlcNAc transferase / similar to O-linked GlcNAc transferase GB:AAB63465 [Caenorhabditis elegans]; supported by cDNA: gi_18139886 Length = 977 Score = 53.5 bits (127), Expect = 8e-08 Identities = 44/197 (22%), Positives = 78/197 (39%) Frame = -3 Query: 592 GTAYAFQRELDSAISDFTKAIQSSPSAGEAWKRRGQARAALGEFKEAIEDLTKALEFEPN 413 G Y +E D I+ +A++ P E + A G+ AI A+E PN Sbjct: 94 GAIYYQLQEYDMCIARNEEALRIQPQFAECYGNMANAWKEKGDTDRAIRYYLIAIELRPN 153 Query: 412 SPDILHERGIVNFKFKDYNSALEDLSTCVKHDKKNSSAHTYLGLTLSALGEYKRAEDEHV 233 D + + A + + + AH+ LG + A G A ++ Sbjct: 154 FADAWSNLASAYMRKGRLSEATQCCQQALSLNPLLVDAHSNLGNLMKAQGLIHEAYSCYL 213 Query: 232 IGLKYDESFLDCWAHLAQLYLDLAYPEKLLNCLEKAIQIDSRFAKAYHLRGILYHGMGRH 53 ++ +F W++LA L+++ + L ++A+++ F AY G +Y +GR Sbjct: 214 EAVRIQPTFAIAWSNLAGLFMESGDLNRALQYYKEAVKLKPAFPDAYLNLGNVYKALGRP 273 Query: 52 RSAIKELSVALTYESSS 2 AI AL +S Sbjct: 274 TEAIMCYQHALQMRPNS 290 >At1g12270.1 hypothetical protein / ; supported by cDNA: gi_17473521 Length = 572 Score = 53.1 bits (126), Expect = 1e-07 Identities = 25/96 (26%), Positives = 49/96 (51%) Frame = -3 Query: 595 RGTAYAFQRELDSAISDFTKAIQSSPSAGEAWKRRGQARAALGEFKEAIEDLTKALEFEP 416 +G + +++ AI +T+AI+ +P+ +A+ R + LG E ++D K +E +P Sbjct: 389 KGNDFFKEQKYPEAIKHYTEAIKRNPNDHKAYSNRAASYTKLGAMPEGLKDAEKCIELDP 448 Query: 415 NSPDILHERGIVNFKFKDYNSALEDLSTCVKHDKKN 308 + V F K+Y++A+E ++HD N Sbjct: 449 TFSKGYSRKAAVQFFLKEYDNAMETYQAGLEHDPSN 484 >At1g62740.1 TPR-repeat protein / contains multiple TPR domains: Pfam profile: PF00515 Length = 571 Score = 52.4 bits (124), Expect = 2e-07 Identities = 25/96 (26%), Positives = 47/96 (48%) Frame = -3 Query: 595 RGTAYAFQRELDSAISDFTKAIQSSPSAGEAWKRRGQARAALGEFKEAIEDLTKALEFEP 416 +G + +++ A+ +T+AI+ +P A+ R LG E ++D K +E +P Sbjct: 388 KGNDFFKEQKYPDAVRHYTEAIKRNPKDPRAYSNRAACYTKLGAMPEGLKDAEKCIELDP 447 Query: 415 NSPDILHERGIVNFKFKDYNSALEDLSTCVKHDKKN 308 +G V F K+Y++A+E ++HD N Sbjct: 448 TFLKGYSRKGAVQFFMKEYDNAMETYQKGLEHDPNN 483 Score = 47.8 bits (112), Expect = 4e-06 Identities = 22/62 (35%), Positives = 34/62 (54%) Frame = -3 Query: 595 RGTAYAFQRELDSAISDFTKAIQSSPSAGEAWKRRGQARAALGEFKEAIEDLTKALEFEP 416 R A+A D A+SD K ++ P G+ + R G A L +F EA+E +K LE +P Sbjct: 42 RSAAHASLNHYDEALSDAKKTVELKPDWGKGYSRLGAAHLGLNQFDEAVEAYSKGLEIDP 101 Query: 415 NS 410 ++ Sbjct: 102 SN 103 >At2g42810.1 putative phosphoprotein phosphatase / ; supported by cDNA: gi_16930440_gb_AF419574.1_AF419574 Length = 484 Score = 50.8 bits (120), Expect = 5e-07 Identities = 27/83 (32%), Positives = 43/83 (51%), Gaps = 5/83 (6%) Frame = -3 Query: 595 RGTAYAFQRELDSAISDFTKAIQSSPSAGEAWKRRGQARAALGEFKEAIEDLTKALEFEP 416 R A+ E SAI D +KAI+ + + RRG A A+G+FK+A++D + P Sbjct: 53 RAFAHTKLEEYGSAIQDASKAIEVDSRYSKGYYRRGAAYLAMGKFKDALKDFQQVKRLSP 112 Query: 415 NSPDILH-----ERGIVNFKFKD 362 N PD E+ ++ KF++ Sbjct: 113 NDPDATRKLKECEKAVMKLKFEE 135 >At4g22670.1 HSP associated protein like / p48, Homo sapiens, PATCHX:G904032;supported by full-length cDNA: Ceres:35786. Length = 441 Score = 50.1 bits (118), Expect = 9e-07 Identities = 33/102 (32%), Positives = 55/102 (53%), Gaps = 4/102 (3%) Frame = -3 Query: 505 AWKRRGQARAAL--GEFKEAIEDLTKALEFEPNSPDILHERGIVNFKFKDYNSALEDLST 332 A + +G+A AL G F EAIE LT+A+ P S + R V K K N+A+ D + Sbjct: 123 AQEAKGKAMEALSEGNFDEAIEHLTRAITLNPTSAIMYGNRASVYIKLKKPNAAIRDANA 182 Query: 331 CVKHDKKNSSAHTYLGLTLSALGEY-KRAEDEHVIG-LKYDE 212 ++ + ++ + G+ + LGE+ + A+D H+ + YDE Sbjct: 183 ALEINPDSAKGYKSRGMARAMLGEWAEAAKDLHLASTIDYDE 224 >At3g17870.1 HSC70-interacting protein, putative / similar to HSC70-INTERACTING PROTEIN GB:P50502 from [Homo sapiens] Length = 281 Score = 48.9 bits (115), Expect = 2e-06 Identities = 33/103 (32%), Positives = 53/103 (51%), Gaps = 4/103 (3%) Frame = -3 Query: 508 EAWKRRGQARAAL--GEFKEAIEDLTKALEFEPNSPDILHERGIVNFKFKDYNSALEDLS 335 +A + +A A+ G F EAIE LTKA+ P S + R V K K N+A+ D + Sbjct: 125 DAQSEKSKAMEAISDGRFDEAIEHLTKAVMLNPTSAILYATRASVFLKVKKPNAAIRDAN 184 Query: 334 TCVKHDKKNSSAHTYLGLTLSALGEYKR-AEDEHVIG-LKYDE 212 ++ + ++ + G+ + LG+++ A D HV L YDE Sbjct: 185 VALQFNSDSAKGYKSRGMAKAMLGQWEEAAADLHVASKLDYDE 227 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,619,117 Number of Sequences: 27288 Number of extensions: 258860 Number of successful extensions: 983 Number of sequences better than 1.0e-05: 16 Number of HSP's better than 0.0 without gapping: 848 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 963 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 948861544 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058376|gb|BM737043.1|BM737043 952052F09.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (605 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g12930.1 expressed protein / ; supported by cDNA: gi_15215... 145 1e-45 At5g62600.1 putative protein / contains similarity to transpo... 47 8e-06 >At1g12930.1 expressed protein / ; supported by cDNA: gi_15215745_gb_AY050402.1_ Length = 1005 Score = 145 bits (366), Expect(2) = 1e-45 Identities = 69/127 (54%), Positives = 95/127 (74%) Frame = +2 Query: 41 ERNRRILRCLLSWVRVRCFSETPAASLARHPLLTFAFNSLQVSFSFEVAIEVMTELVSQH 220 + NR+ILRCLLSWVR CFSE P ++ HPLL + FN+LQ + +F++AIEV+ ELV++H Sbjct: 198 DNNRKILRCLLSWVRAGCFSEIPQGAVPSHPLLNYVFNALQGT-TFDLAIEVLVELVTRH 256 Query: 221 QELPEAFLSKTPYIREALLLPALANRNEKIIAGLACLMCEVGQAAPALVAEGGSQALALT 400 ++LP+ L K ++R+ LL PAL N + KII+GLACLM E+GQAAP L+ E S+AL LT Sbjct: 257 EDLPQVLLYKVQFLRDTLLKPALINADLKIISGLACLMSEIGQAAPCLIVEASSEALILT 316 Query: 401 DGLLRSI 421 D +L + Sbjct: 317 DAILSCV 323 Score = 55.1 bits (131), Expect(2) = 1e-45 Identities = 27/56 (48%), Positives = 38/56 (67%), Gaps = 1/56 (1%) Frame = +3 Query: 441 CVAFTSEDWEIAESTLQFWCSLAHFILGI-DVKSAKRNVAQELLGPVFSSLLDALL 605 CV F SEDWEIA+ST+QFW + A +IL + + R ++ PVFS+L+DAL+ Sbjct: 322 CVTFPSEDWEIADSTVQFWSTFATYILSLGGNRQNDRTRVKDTFLPVFSALVDALV 377 >At5g62600.1 putative protein / contains similarity to transportin-SR Length = 572 Score = 47.0 bits (110), Expect = 8e-06 Identities = 34/137 (24%), Positives = 67/137 (48%), Gaps = 11/137 (8%) Frame = +2 Query: 35 LDERNRRILRCLLSWVRVRCFSETPAASLARHPLLTFAFNSLQVSFSFEVAIEVMTELVS 214 + E ++L SW+R+R P LA HPL+ A +SL E ++ V++EL+ Sbjct: 195 ISELKEQVLEAFASWLRLR--HGIPGTVLACHPLVHAALSSLNCDPLSEASVNVISELI- 251 Query: 215 QHQELPEA--FLSKTPYIREALLLPAL---------ANRNEKIIAGLACLMCEVGQAAPA 361 H P + ++TP I+ +++P + ++++E+ + + L +VG + Sbjct: 252 HHTASPSSGGISAQTPLIQ--VIVPQILSLQAHLRDSSKDEEDVKAIGRLFADVGDSYVE 309 Query: 362 LVAEGGSQALALTDGLL 412 L+A G + + + LL Sbjct: 310 LIATGSDEPMVIVHALL 326 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,472,925 Number of Sequences: 27288 Number of extensions: 281374 Number of successful extensions: 715 Number of sequences better than 1.0e-05: 4 Number of HSP's better than 0.0 without gapping: 691 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 713 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 957813068 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058329|gb|BM736996.1|BM736996 952051H12.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (566 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g24260.1 dipeptidyl peptidase IV-like protein / 218 2e-57 >At5g24260.1 dipeptidyl peptidase IV-like protein / Length = 746 Score = 218 bits (554), Expect = 2e-57 Identities = 101/167 (60%), Positives = 130/167 (77%), Gaps = 1/167 (0%) Frame = -3 Query: 564 PFAGAATVKVRLGVVHSHGG-EVTWMDLLCGDPNGPHSDEEYLARVNWMHNSALAVQVLN 388 PFAGA +RLGVV S GG + TWM+L+CG ++++EYL RVNW+ + L VQVLN Sbjct: 244 PFAGALNSTLRLGVVSSAGGGKTTWMNLVCGGRG--NTEDEYLGRVNWLPGNVLIVQVLN 301 Query: 387 RSHTKLKLLKFDISTGEREVLLEEQHDVWITLHDCFTPLDKGVNNKHPGGFIWASEKTGF 208 RS +KLK++ FDI+TG+ VLL E+ D W+TLHDCFTPL+K +++ GGFIWASE+TGF Sbjct: 302 RSQSKLKIISFDINTGQGNVLLTEESDTWVTLHDCFTPLEKVPSSRGSGGFIWASERTGF 361 Query: 207 RHLYVHGNDGACLGPLTQGDWMVEHIAGINESNGLIYFTGTLDGPLE 67 RHLY++ +DG CLG +T G+WMVE IAG+NE L+YFTGTLDGPLE Sbjct: 362 RHLYLYESDGTCLGAITSGEWMVEQIAGVNEPMSLVYFTGTLDGPLE 408 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,139,168 Number of Sequences: 27288 Number of extensions: 287805 Number of successful extensions: 759 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 732 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 757 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 841443256 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058314|gb|BM736981.1|BM736981 952051G03.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (209 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g60360.1 putative protein / CGI-94 protein, Homo sapiens, ... 87 1e-18 >At3g60360.1 putative protein / CGI-94 protein, Homo sapiens, EMBL:AF151852;supported by full-length cDNA: Ceres:13387. Length = 228 Score = 87.4 bits (215), Expect = 1e-18 Identities = 40/56 (71%), Positives = 49/56 (87%) Frame = +3 Query: 33 MSSLRNAIPRRAHKERAQPEARKKVGLLEKHKDYVVRARAYHIKEATIAKLKEKAA 200 MSSLRNAIPR AHKER+QPEARK+ G+LEKHKDY++RA AYH K+ T+ L++KAA Sbjct: 1 MSSLRNAIPRPAHKERSQPEARKRFGILEKHKDYIIRANAYHKKQETLKILRQKAA 56 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,756,649 Number of Sequences: 27288 Number of extensions: 54208 Number of successful extensions: 135 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 134 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 135 length of database: 11,516,596 effective HSP length: 45 effective length of database: 10,288,636 effective search space used: 246927264 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058310|gb|BM736977.1|BM736977 952051F08.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (569 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g80460.1 putative glycerol kinase / similar to GLYCEROL KI... 283 5e-78 >At1g80460.1 putative glycerol kinase / similar to GLYCEROL KINASE GB:O69664 from [Mycobacterium tuberculosis] Length = 522 Score = 283 bits (724), Expect(2) = 5e-78 Identities = 131/171 (76%), Positives = 150/171 (87%) Frame = +2 Query: 56 YDRHAKPGASHQLEFKQHYPEAGWVEHDPMEIIETVKVCMKDAVGKAKAGKHNVVAGLKA 235 YD A+P ASHQ+EF Q YPEAGWVEHDPMEI+E+VKVC+ A+ KA A HNV GLKA Sbjct: 23 YDHDARPVASHQVEFTQFYPEAGWVEHDPMEILESVKVCIAKALDKATADGHNVDGGLKA 82 Query: 236 IGITNQRETTVMWSKSTGRPLNNAIVWMDARTSPVCRRLENELSGGRTHFVETCGLPIST 415 IG+T+QRETTV+WSKSTG PL+ AIVWMDARTS +CRRLE ELSGGR+HFVE+CGLPIST Sbjct: 83 IGLTDQRETTVVWSKSTGLPLHKAIVWMDARTSSICRRLEKELSGGRSHFVESCGLPIST 142 Query: 416 YFSALKLLWLMENVDAVKDAVRTGDALFGTIDTWLIWNLTGGVAGGQHVTD 568 YFSA+KLLWLMENVD VKDA++ GDA+FGTIDTWLIWN+TGG+ GG HVTD Sbjct: 143 YFSAMKLLWLMENVDDVKDAIKKGDAIFGTIDTWLIWNMTGGINGGLHVTD 193 Score = 25.4 bits (54), Expect(2) = 5e-78 Identities = 10/16 (62%), Positives = 13/16 (80%) Frame = +1 Query: 1 EEVYVAAIDQGTTSTR 48 E ++ +IDQGTTSTR Sbjct: 4 ENGFIGSIDQGTTSTR 19 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,518,867 Number of Sequences: 27288 Number of extensions: 339894 Number of successful extensions: 812 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 784 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 812 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 850394780 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058179|gb|BM736846.1|BM736846 952049G08.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (299 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g27325.1 hypothetical protein / similar to negative regula... 108 1e-25 >At3g27325.1 hypothetical protein / similar to negative regulator of COPII vesicle formation GB:NP_011162 from [Saccharomyces cerevisiae] Length = 1048 Score = 108 bits (270), Expect(2) = 1e-25 Identities = 47/63 (74%), Positives = 58/63 (91%) Frame = +3 Query: 108 GGIHDYQIRSRVASLDGIVPSTHGFMVGSSSMKNVWLSMEHQSILWCNQLAVQVAHTLLS 287 GG +DYQ+RS++ SLDGIVPS+HGFM+ S+SM NVWLSMEHQ+ILWCNQL VQV+HTLLS Sbjct: 305 GGYNDYQVRSKLESLDGIVPSSHGFMISSTSMTNVWLSMEHQAILWCNQLVVQVSHTLLS 364 Query: 288 IID 296 ++D Sbjct: 365 MVD 367 Score = 23.1 bits (48), Expect(2) = 1e-25 Identities = 7/12 (58%), Positives = 10/12 (83%) Frame = +1 Query: 13 SHVNEEWRKGYK 48 + VN EW+KGY+ Sbjct: 269 AQVNREWKKGYE 280 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,579,970 Number of Sequences: 27288 Number of extensions: 119859 Number of successful extensions: 208 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 204 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 208 length of database: 11,516,596 effective HSP length: 75 effective length of database: 9,469,996 effective search space used: 227279904 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058175|gb|BM736842.1|BM736842 952049F12.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (561 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g64570.1 hypothetical protein / predicted by genscan+ 167 3e-42 >At1g64570.1 hypothetical protein / predicted by genscan+ Length = 1239 Score = 167 bits (424), Expect = 3e-42 Identities = 80/117 (68%), Positives = 95/117 (80%) Frame = -2 Query: 395 LLALGILEYNNDWAAIQKRFLPCKSKHQIFVSQKNRSSSKAPDNPVKDVRHMKTSPLTDE 216 LLALGI+EYN+DW AI++RFLP K +HQI+V QKNR SSKAP+NP+K V MK+SPLT + Sbjct: 511 LLALGIMEYNSDWKAIKQRFLPSKGEHQIYVRQKNRRSSKAPENPIKAVLRMKSSPLTPQ 570 Query: 215 EKECIQEGLRIFKNDWTSVWRFVVPHRDPSLLQRQWRVASGIQKSYTKSDAEKERRR 45 E IQEGL+ FK DWTSVW+FVVP+RDPSLL RQWR A GIQKSY +KE+RR Sbjct: 571 EILRIQEGLKYFKYDWTSVWKFVVPYRDPSLLPRQWRTALGIQKSYKLDAVKKEKRR 627 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,794,653 Number of Sequences: 27288 Number of extensions: 284386 Number of successful extensions: 901 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 866 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 901 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 835029516 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058079|gb|BM736746.1|BM736746 952048D04.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (544 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g60740.1 beta-tubulin cofactor - like protein / beta-tubul... 199 6e-52 >At3g60740.1 beta-tubulin cofactor - like protein / beta-tubulin folding cofactor D, Homo sapiens, EMBL:HSA6417; supported by cDNA: gi_20514262 Length = 1254 Score = 199 bits (507), Expect = 6e-52 Identities = 100/181 (55%), Positives = 136/181 (74%), Gaps = 1/181 (0%) Frame = +3 Query: 3 HQEHLIPFIPHRELLEEIIPNSRDLEWAVPTVSYPRLVKLLQVSYYSKSVLSGLVISTGG 182 H+ +P +P+RE LEEI+PN +L+WAVP S+PR V+LL++ YSK V+SGLVIS GG Sbjct: 964 HKSVSVPHVPYREKLEEILPNKANLQWAVPAFSFPRFVQLLKLRCYSKEVMSGLVISIGG 1023 Query: 183 LQESLKKASMSALVGYLQDSDINTNCEGKSREYLLSCDLLWGLQHYQKCDRVITPMFKTI 362 LQ+SL+KAS+ AL+ Y+++ + E +SRE L D+LW LQ Y+KCDRV+ P +TI Sbjct: 1024 LQDSLRKASLVALLEYMREGEAKDPKEQQSRESALGDDILWILQEYKKCDRVMVPCLQTI 1083 Query: 363 EALFSKKVFLNKEGYS-EFYSGLVDSVSSELKGSKDFTKLCAGLSILGYISSESDGTCTK 539 E LFS K+FLN+E Y+ FY+G++DS++ EL+ SKDFTKL AGL+ILGYI+S S TK Sbjct: 1084 EILFSSKIFLNQESYTFSFYAGVMDSLAIELRASKDFTKLKAGLAILGYIASVSHFISTK 1143 Query: 540 A 542 A Sbjct: 1144 A 1144 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,779,899 Number of Sequences: 27288 Number of extensions: 237791 Number of successful extensions: 630 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 619 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 629 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 781156644 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19058073|gb|BM736740.1|BM736740 952048C10.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (574 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g20190.1 unknown protein / ; supported by cDNA: gi_20259451 80 7e-16 >At2g20190.1 unknown protein / ; supported by cDNA: gi_20259451 Length = 1330 Score = 80.1 bits (196), Expect = 7e-16 Identities = 67/187 (35%), Positives = 90/187 (47%), Gaps = 1/187 (0%) Frame = -1 Query: 574 GTSAIVAMDKXXXXXXXXXXXSNTLRLSQSKTIGXXXXXXXXXXXXXXXXXXSAIESLLK 395 GTSAIVAMD+ S L LSQSK + SAIES+L+ Sbjct: 429 GTSAIVAMDRSSNLSSGGSLSSGLL-LSQSKDVNKGSERSLESVLQSSKQKVSAIESMLR 487 Query: 394 GVSMSG-QNFSAARSTSLDLGXXXXXXXXXXXPLAAPASNVLSLQNSALLDSSLPTIPPS 218 G+ +S QN +A RS+SLDLG APASN S +SA +S+ S Sbjct: 488 GLHISDRQNPAALRSSSLDLGVDPPSSRDPPFHAVAPASN--SHTSSAAAESTHSINKGS 545 Query: 217 SRNGGSRLLDTMTTHLPTKERSRSPYLSNMSSESISGLSLPYSRKSLDRLQEGGRMDESY 38 +RNGG L D +T +K+ RS Y N+ SES S +++ +R E ++ES Sbjct: 546 NRNGGLGLSDIITQIQASKDSGRSSYRGNLLSESHPTFSSLTAKRGSER-NERSSLEESN 604 Query: 37 DIRSTRQ 17 D R R+ Sbjct: 605 DAREVRR 611 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,239,527 Number of Sequences: 27288 Number of extensions: 214243 Number of successful extensions: 624 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 611 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 621 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 859346304 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19057997|gb|BM736664.1|BM736664 952047A12.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (406 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g18410.1 putative protein / p53 inducible protein PIR121, ... 175 8e-45 >At5g18410.1 putative protein / p53 inducible protein PIR121, Homo sapiens, EMBL:AF160973 Length = 1235 Score = 175 bits (443), Expect = 8e-45 Identities = 85/122 (69%), Positives = 100/122 (81%) Frame = +2 Query: 8 VGDSVAWAGCTIMYLLGQQQHFELFDFSYQFLNVAEVESATVSHYQSSERTKSSNFLQGY 187 +GDS+AW GCTI+YLLGQQ HFELFDFSYQ LNV+EVE+ + SH ++ QG+ Sbjct: 1117 LGDSIAWGGCTIIYLLGQQLHFELFDFSYQVLNVSEVETVSASHTH-----RNPQIHQGW 1171 Query: 188 EGILEAMRKARRLNNHVFSMLRARCPLEDKVACAIKPSGAPLHRMKFMNTVSAFETLPQR 367 EG+LE M+KARRLNNHVFSML+ARCPLEDK ACAIK SGAPL R++F NTVSAFETLPQ+ Sbjct: 1172 EGLLEGMKKARRLNNHVFSMLKARCPLEDKTACAIKQSGAPLPRVRFENTVSAFETLPQK 1231 Query: 368 VT 373 T Sbjct: 1232 GT 1233 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,777,029 Number of Sequences: 27288 Number of extensions: 172035 Number of successful extensions: 471 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 462 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 470 length of database: 11,516,596 effective HSP length: 88 effective length of database: 9,115,252 effective search space used: 419301592 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19057936|gb|BM736603.1|BM736603 952038G05.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (380 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g51740.1 putative protein / contains similarity to peptidase 189 5e-49 >At5g51740.1 putative protein / contains similarity to peptidase Length = 485 Score = 189 bits (479), Expect = 5e-49 Identities = 95/126 (75%), Positives = 112/126 (88%) Frame = +2 Query: 2 NKFRADAEVATVVGHEGGHAIARHSAEQITKNLWVAILQIGILQFIYMPDLINTGSTLLL 181 N F++DAEVATV+GHE GHA+ARH AE ITKNLW AILQ+ + QF+ MPDL+NT S L L Sbjct: 338 NHFKSDAEVATVIGHEVGHAVARHVAEGITKNLWFAILQLVLYQFV-MPDLVNTMSALFL 396 Query: 182 RLPFSRRMEIEADHIGLLLLAAAGYDPRVAPSVYEKLGKIGGDSALNNYLSTHPSSKKRA 361 RLPFSR+MEIEAD+IGLLLLA+AGYDPRVAP+VYEKLGK+GGD AL +YLSTHPS KKR+ Sbjct: 397 RLPFSRKMEIEADYIGLLLLASAGYDPRVAPTVYEKLGKLGGD-ALGDYLSTHPSGKKRS 455 Query: 362 QLLSQA 379 +LL+QA Sbjct: 456 KLLAQA 461 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,376,148 Number of Sequences: 27288 Number of extensions: 136941 Number of successful extensions: 393 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 389 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 391 length of database: 11,516,596 effective HSP length: 87 effective length of database: 9,142,540 effective search space used: 356559060 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19057773|gb|BM736440.1|BM736440 952050G10.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (518 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g21110.1 phosphoribosyamidoimidazole-succinocarboxamide synth... 267 2e-72 >At3g21110.1 phosphoribosyamidoimidazole-succinocarboxamide synthase, putative / similar to PHOSPHORIBOSYLAMIDOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE GB:P38025 from [Arabidopsis thaliana];supported by full-length cDNA: Ceres:35508. Length = 411 Score = 267 bits (683), Expect = 2e-72 Identities = 124/168 (73%), Positives = 149/168 (87%) Frame = +2 Query: 14 NQTRHITPNAVVSSPDKNVTIAKRCTVFPVEFVVRGFVTGSTDTSLWTGYNKGVRNYCGN 193 N T+HITPNA+VSSPD+NV IAK+C+VFP+EFVVRG+VTGSTDTSLWT YNKGVRNYCGN Sbjct: 158 NNTQHITPNAIVSSPDRNVVIAKKCSVFPIEFVVRGYVTGSTDTSLWTVYNKGVRNYCGN 217 Query: 194 VLPDGMVKNEKLSSNILTPTTKAADHDVPVTPDEIIESGLMSKEDFHEAGSKALSLFAYG 373 L DG+VKN+KL +NILTPTTKAADHDVP++P+EI+E G M++ +F EA KALSLF +G Sbjct: 218 ELSDGLVKNQKLPANILTPTTKAADHDVPISPNEIVEGGFMTQAEFDEASMKALSLFEFG 277 Query: 374 QKVALENGLILVDTKYEFGKTPDGTIGLIDEGHTPDSSRYWIANSYED 517 Q VA ++GLILVDTKYEFG++ DG+I LIDE HTPDSSRYW+A SYE+ Sbjct: 278 QGVAKKHGLILVDTKYEFGRSSDGSILLIDEIHTPDSSRYWLAGSYEE 325 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,566,330 Number of Sequences: 27288 Number of extensions: 210493 Number of successful extensions: 554 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 546 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 554 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 720488000 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|19057755|gb|BM736422.1|BM736422 952050E05.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (609 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g33945.1 putative protein / ; supported by cDNA: gi_20466... 188 2e-48 >At4g33945.1 putative protein / ; supported by cDNA: gi_20466737 Length = 464 Score = 188 bits (478), Expect = 2e-48 Identities = 92/194 (47%), Positives = 128/194 (65%) Frame = +1 Query: 28 KSNVQSLYDAIRVLLKPDDNRVVASQVYGYSRRFAEIGVAEVLVNALREQVXXXXXXXXX 207 K+ +++LYDAI LL PDD RVVASQVYGY+R FA++G+A L AL+ + Sbjct: 219 KTTIRALYDAICALLTPDDFRVVASQVYGYARTFAKLGIATALTEALQAGIGSDSLVSAS 278 Query: 208 XXXXXIAVNDEICRSISENGGIDVLLQCIDGAGEQKNKAVARXXXXXXXXXXXXXXXXXI 387 IAVNDEIC+SI+E+GGID LL+CID +GEQ NK A+ Sbjct: 279 TALKAIAVNDEICKSIAESGGIDTLLRCIDDSGEQGNKTAAKTCCSLLSKLAGSDSNKST 338 Query: 388 IIQRGGFDRFLKLTSRFSEDPAIIQEVMSMVTVLTLRSPENAARAMEEGYGTLAIQAMQR 567 I+++ G D+ + L RFS+DP +IQEVM +++++ LRSP++AA+A+E G G LA+QAM+R Sbjct: 339 IVEKQGLDKLITLALRFSDDPLVIQEVMLIISIICLRSPDHAAKAIEAGAGDLAVQAMKR 398 Query: 568 FPSSVQTQKQACLM 609 FP + Q Q+ AC M Sbjct: 399 FPVAAQMQRNACNM 412 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,556,309 Number of Sequences: 27288 Number of extensions: 293690 Number of successful extensions: 781 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 766 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 781 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 966764592 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18965232|gb|BM661467.1|BM661467 952048G12.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (424 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g05570.1 putative protein / similar to unknown protein (pi... 151 1e-37 >At5g05570.1 putative protein / similar to unknown protein (pir||T04661) Length = 1124 Score = 151 bits (382), Expect = 1e-37 Identities = 77/139 (55%), Positives = 98/139 (70%) Frame = -2 Query: 420 LQFITNQGLLVAISNENEIQVWNLEFRQLFYSSQWDVNITAFAVIERTFMMYLGDENGLL 241 L+F+ NQG LV+ISNENEIQVW+L+ RQ S +W+ NITAFA++ T MY+GDE G++ Sbjct: 91 LEFMQNQGFLVSISNENEIQVWDLDLRQPASSLKWESNITAFAILHGTGYMYVGDEYGMV 150 Query: 240 SVLKYDVDDGKLQIMPYNVHIHSLIERSGVSLQDPQPIVGILIQPDTFGTRLLIAYERGL 61 SVL Y D+GKL +PY V +L E +G+S P+VG+L QP + GTRLLIA+ GL Sbjct: 151 SVLNYSADEGKLLQLPYYVPTDALSEAAGLSSPIDYPVVGLLSQPCSKGTRLLIAFSNGL 210 Query: 60 LVLWDVSEDRAVSVRGYGD 4 L LWD SED V VRG D Sbjct: 211 LFLWDASEDHVVLVRGNKD 229 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,432,778 Number of Sequences: 27288 Number of extensions: 189861 Number of successful extensions: 575 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 560 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 575 length of database: 11,516,596 effective HSP length: 89 effective length of database: 9,087,964 effective search space used: 463486164 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18965199|gb|BM661448.1|BM661448 952048E07.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (585 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g15180.1 expressed protein / ;supported by full-length cDN... 129 8e-31 >At3g15180.1 expressed protein / ;supported by full-length cDNA: Ceres:39566. Length = 519 Score = 129 bits (325), Expect = 8e-31 Identities = 61/149 (40%), Positives = 96/149 (63%), Gaps = 1/149 (0%) Frame = +3 Query: 141 DFASYPGLHSDDTGREFLERCPLPKLLGALQSEEDVPGMVETVTECLDKIFSSRYGASLL 320 +FA YPG ++ + +EFL+R PLP + ALQ++ D+PG T+ CL+++F ++YGASL+ Sbjct: 13 EFAHYPGAQNETSVKEFLDRFPLPVIFNALQTDPDIPGFENTLVTCLERLFKTKYGASLI 72 Query: 321 PNYGAFIQAGLHTDSKQIRKLACKAVLHLLDKAEDGG-AAVETVVQHNLYPLLIKCLIEG 497 P Y +Q GL DS ++ LACK VL LL+ + ++V+ VV + +YPLL+ +I Sbjct: 73 PQYMPVLQVGLKADSAVVKSLACKTVLCLLEDCDTNDVSSVQLVVNNGIYPLLLDYIINS 132 Query: 498 DEEISAIILDTGKRLAQIPKGAEVIFPPD 584 D+E++ +T K LA+ P VIFP + Sbjct: 133 DDEVANAASETIKSLARFPDAMSVIFPSE 161 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,919,896 Number of Sequences: 27288 Number of extensions: 269870 Number of successful extensions: 815 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 790 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 814 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 895152400 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18965131|gb|BM661409.1|BM661409 952047H04.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (285 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g56730.1 zinc protease PQQL-like protein / 132 3e-32 >At5g56730.1 zinc protease PQQL-like protein / Length = 956 Score = 132 bits (333), Expect = 3e-32 Identities = 61/94 (64%), Positives = 84/94 (88%) Frame = +1 Query: 1 EISIVRALMMSEMESAYLERDQMQSTSLRDEFLQHFLREEPVVGIEYEAQLQKTLLPHIS 180 EIS+VRALMMSE+ESAYLERDQ+QSTSLRDE++QHFL +EPV+GIEYEAQLQKTLLP IS Sbjct: 358 EISVVRALMMSEIESAYLERDQVQSTSLRDEYIQHFLHKEPVIGIEYEAQLQKTLLPQIS 417 Query: 181 SAEVAKFAENFSTASSCVIKIVEPRAHASLEDLK 282 +++V++++E T+ CVIK +EP++ A+++ ++ Sbjct: 418 ASDVSRYSEKLRTSCGCVIKSMEPKSAATIDHMR 451 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.316 0.128 0.333 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,913,993 Number of Sequences: 27288 Number of extensions: 106934 Number of successful extensions: 344 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 342 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 344 length of database: 11,516,596 effective HSP length: 70 effective length of database: 9,606,436 effective search space used: 230554464 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18965050|gb|BM661364.1|BM661364 952047B05.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (537 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g42440.1 expressed protein / ; supported by cDNA: gi_13605... 218 2e-57 At1g06720.1 unknown protein / 50 7e-07 >At1g42440.1 expressed protein / ; supported by cDNA: gi_13605689_gb_AF361826.1_AF361826 Length = 793 Score = 218 bits (555), Expect = 2e-57 Identities = 108/167 (64%), Positives = 132/167 (78%), Gaps = 2/167 (1%) Frame = +2 Query: 41 FPDEVETPLDVPAKKRFAKYRGLKSFRTSSWDPKESLPLDYARIFAFDNFTRTQKHVFAK 220 FPDEVETP+DVPA++RFAKYRGLKSFRTSSWDP ESLP DYARIFAFDN RTQK V + Sbjct: 481 FPDEVETPIDVPARRRFAKYRGLKSFRTSSWDPNESLPQDYARIFAFDNVARTQKLVLKQ 540 Query: 221 IAEL-DGGMKNCAQVGSYVRLYVKNVPTDIASKLCH-PSRRIPVVVSGLLQHESKMSVLH 394 ++ + +C +GSYVRL++K VP ASKL + P++ GLLQHESKMSVLH Sbjct: 541 ALKMEEEDRDDCVPIGSYVRLHIKEVPLGAASKLSSLVNTTKPIIGFGLLQHESKMSVLH 600 Query: 395 FSIKKHDSYEAPIKSKEPLIFNVGFRQFTARPLXSSDNINCNKHKME 535 FS+KK+D YEAPIK+KE L+F+VGFRQF ARP+ ++DN + +KHKME Sbjct: 601 FSVKKYDGYEAPIKTKEELMFHVGFRQFIARPVFATDNFSSDKHKME 647 >At1g06720.1 unknown protein / Length = 1147 Score = 50.1 bits (118), Expect = 7e-07 Identities = 25/94 (26%), Positives = 55/94 (57%), Gaps = 2/94 (2%) Frame = +2 Query: 257 QVGSYVRLYVKNVPTDIASKL--CHPSRRIPVVVSGLLQHESKMSVLHFSIKKHDSYEAP 430 + G+Y+RL + NVP ++ CHP ++V G+ E + + +KKH ++ Sbjct: 751 RTGTYLRLEIHNVPYEMVEFFDPCHP-----ILVGGIGFGEDNVGYMQARLKKHRWHKKV 805 Query: 431 IKSKEPLIFNVGFRQFTARPLXSSDNINCNKHKM 532 +K+++P+I ++G+R++ P+ + ++ N +H+M Sbjct: 806 LKTRDPIIVSIGWRRYQTIPVFAIEDRN-GRHRM 838 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,692,723 Number of Sequences: 27288 Number of extensions: 215731 Number of successful extensions: 565 Number of sequences better than 1.0e-05: 4 Number of HSP's better than 0.0 without gapping: 552 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 562 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 763199020 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18965006|gb|BM661336.1|BM661336 952046H04.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (476 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g64420.1 putative protein / similar to unknown protein (pi... 114 2e-26 >At5g64420.1 putative protein / similar to unknown protein (pir||T00246) Length = 1306 Score = 114 bits (286), Expect = 2e-26 Identities = 61/156 (39%), Positives = 97/156 (62%), Gaps = 9/156 (5%) Frame = +3 Query: 33 SRYNTVASVAQNATFWILKIINSKHCSEQELARVVDLFRSILSDY-DRKKSRLKLGFVKQ 209 +RY + + QN+T+W++KII+S+ SE EL +++D+FRS ++ Y D +KS+LK+ F+++ Sbjct: 1113 NRYKMITHLGQNSTYWVMKIIDSRKFSETELEKILDVFRSAVTGYFDSRKSQLKIDFLEE 1172 Query: 210 VAKRNPWIGQKLFGFVLQRAENTKAQYRRNQLLELAEFILKS--------WADGTSEMFL 365 V +R PWIG +LFGF+L+ + N ++RR + L+L L+S AD M Sbjct: 1173 VFRRRPWIGHQLFGFLLEASVNANVEFRRLEALDLITETLRSLIPINENTQADSRKTM-T 1231 Query: 366 NHLSQLCGLIQAALSAVAENKSRRREVRNFCTGILQ 473 HL +L LI+ + + E K RR +VR FC I Q Sbjct: 1232 THLKELILLIKELVGNMPEAKVRRAQVRKFCGRIFQ 1267 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,605,201 Number of Sequences: 27288 Number of extensions: 180519 Number of successful extensions: 619 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 608 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 617 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 605236996 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18965004|gb|BM661335.1|BM661335 952046H02.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (448 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g62360.1 putative protein / Pm5 protein - Homo sapiens, PI... 153 3e-38 >At3g62360.1 putative protein / Pm5 protein - Homo sapiens, PIR:S21977 Length = 1227 Score = 153 bits (387), Expect = 3e-38 Identities = 78/136 (57%), Positives = 91/136 (66%), Gaps = 2/136 (1%) Frame = +3 Query: 42 PATSDVDIKILAAGKSNYAHLNKGDVATEAKTDSEGSFFAGPLYDDIVYKVEASKDGYHL 221 P V+IKI AA S + L KG++A E T S GSF AGPLYDDI Y EASK GYH+ Sbjct: 811 PPLPGVNIKIFAAKDSLISSLKKGEIAIETSTLSAGSFVAGPLYDDIPYATEASKPGYHI 870 Query: 222 KQTGPYTFSCQKLGQILVRIYGENXXXXXX--XXXXXXGEKGYRNNSISSSGGTFTFDNL 395 K+ GPY+FSCQKLGQI VR+ ++ G+ GYRNNSIS +GG F FD+L Sbjct: 871 KRLGPYSFSCQKLGQISVRVNSKDNAETSIPPLLLSLSGDHGYRNNSISGAGGLFVFDSL 930 Query: 396 FPGXFYLRPLLKEYKF 443 FPG FYLRPLLKEY F Sbjct: 931 FPGNFYLRPLLKEYSF 946 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,149,409 Number of Sequences: 27288 Number of extensions: 176865 Number of successful extensions: 390 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 386 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 389 length of database: 11,516,596 effective HSP length: 90 effective length of database: 9,060,676 effective search space used: 525519208 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18964932|gb|BM661293.1|BM661293 952046E10.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (455 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g18730.1 hypothetical protein / predicted by genscan+ 167 4e-43 >At3g18730.1 hypothetical protein / predicted by genscan+ Length = 1220 Score = 167 bits (424), Expect(2) = 4e-43 Identities = 81/133 (60%), Positives = 105/133 (78%), Gaps = 1/133 (0%) Frame = +1 Query: 55 KKYFKSSMKLAWILKERSP-SQKSDFLKELIDAYNNMGMLELELDNFEEAEKLLIQGLKI 231 KKYFK +M+LA ILKE+ P + S FL+E I+A+NN+GML+L+LDN E A +L +GL+I Sbjct: 134 KKYFKKAMELAQILKEKPPPGESSGFLEEYINAHNNIGMLDLDLDNPEAARTILKKGLQI 193 Query: 232 CDDEEVDSYDDARTRLHHNLGNVYTELQNWKKARGHIEKDIEICRKIRHLQGEAKGFINL 411 CD+EEV YD AR+RLHHNLGNV+ L++W +A+ HIE DI IC KI H+QGEAKG+INL Sbjct: 194 CDEEEVREYDAARSRLHHNLGNVFMALRSWDEAKKHIEMDINICHKINHVQGEAKGYINL 253 Query: 412 GELHYRVQKYDNA 450 ELH + QKY +A Sbjct: 254 AELHNKTQKYIDA 266 Score = 23.5 bits (49), Expect(2) = 4e-43 Identities = 10/19 (52%), Positives = 13/19 (67%) Frame = +3 Query: 3 YHEILLRSENDHSAIGMQK 59 YHE+ L+SE+D AI K Sbjct: 116 YHEMFLKSEDDCEAIQSAK 134 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,642,234 Number of Sequences: 27288 Number of extensions: 195064 Number of successful extensions: 587 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 566 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 584 length of database: 11,516,596 effective HSP length: 90 effective length of database: 9,060,676 effective search space used: 552701236 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18964413|gb|BM660991.1|BM660991 952043E06.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (611 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g19490.1 putative protein / probable membrane protein YDR0... 205 2e-53 >At4g19490.1 putative protein / probable membrane protein YDR027c, Saccharomyces cerevisiae, PIR2:S50934 Length = 939 Score = 205 bits (521), Expect = 2e-53 Identities = 110/216 (50%), Positives = 149/216 (68%), Gaps = 13/216 (6%) Frame = +3 Query: 3 KASIKTTVGELLPVLTARPIDSDSVTGDRATDADA---------GGQSLANKLRSLSSEG 155 K +IK V +LLP+L AR ++SD G+R+ D + GG SLA+KLR+LSSE Sbjct: 420 KNAIKKAVADLLPILVARSLESDFSHGERSVDDETILTPSAYTGGGLSLASKLRTLSSEA 479 Query: 156 FVQLLSAIFRIVQVHLQQAAEVKRIVEWVMGNLDGTLGVDASNPTLQHGGTVIPDTQEND 335 FV LL+AIF+IVQ HL +A+EVK+ +EW++ N+DG D+ + G QE Sbjct: 480 FVNLLTAIFKIVQAHLVRASEVKKAIEWILCNIDGHYAADSVAAAIAVGAVAAETAQEIG 539 Query: 336 SSRGS---NTLTRSTSKIPFVQGKTNDFS-IINSLKNVRADVLRENTEAVFAACDAAHGR 503 GS + L ++TSK P +QGK++D S ++N +N RADVLRENTEAVFAAC+ HGR Sbjct: 540 FQGGSLVSSPLGKATSKAPPLQGKSSDASSLMNMSRNFRADVLRENTEAVFAACEVTHGR 599 Query: 504 WAKLLGVRSALHPRLRLQEFLIIYNITEEFIAATEK 611 WAKLLGVR+ LHP+L+LQEF+ IY++T+EFI +TEK Sbjct: 600 WAKLLGVRALLHPKLKLQEFMSIYDLTQEFITSTEK 635 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,790,972 Number of Sequences: 27288 Number of extensions: 228237 Number of successful extensions: 619 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 607 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 616 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 963817488 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18964362|gb|BM660962.1|BM660962 952043C09.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (465 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g72550.1 putative phenylalanyl-tRNA synthetase beta-subunit; ... 167 2e-42 >At1g72550.1 putative phenylalanyl-tRNA synthetase beta-subunit; PheHB / similar to phenylalanyl-tRNA synthetase beta-subunit (PheHB) GB:5032011 [Homo sapiens]; supported by cDNA: gi_17065261 Length = 598 Score = 167 bits (423), Expect = 2e-42 Identities = 85/137 (62%), Positives = 102/137 (74%), Gaps = 1/137 (0%) Frame = +2 Query: 2 RNVFIECTATDLTKANIVLNTMVTMFSEYCETKFEVEPVEVIHHDGRKTVYPDLSCYKMD 181 +NVFIECTATDLTKA IVLNTMVT FSE+C KFE+EPVEV + DG+ +YPDL+ Y M+ Sbjct: 250 KNVFIECTATDLTKAKIVLNTMVTTFSEFCARKFEIEPVEVTYDDGKSYIYPDLAVYDME 309 Query: 182 VLLSDILGPIGISQDEKQVVCLLNKMQLQAE-SHSLKGEARISVSVPPTRSDILHARDLS 358 V LS I IG+S +QV LL +MQLQAE + S + I V VPP+RSD+LH D+ Sbjct: 310 VPLSFITDSIGVSLKVEQVTSLLTRMQLQAEQAKSSDNQCAIKVHVPPSRSDVLHPCDVM 369 Query: 359 EDVAIAYGFNNVPKSKP 409 EDVAIAYGFNN+P KP Sbjct: 370 EDVAIAYGFNNIPTRKP 386 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,146,777 Number of Sequences: 27288 Number of extensions: 213655 Number of successful extensions: 575 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 564 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 574 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 569103444 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18964171|gb|BM660851.1|BM660851 952042D07.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (488 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g26780.1 unknown protein / similar to GP|522127|gnl|PID|e3... 193 9e-58 >At2g26780.1 unknown protein / similar to GP|522127|gnl|PID|e349073|Z35639 and GP|2224677|gnl|PID|d1021664|AB002366; supported by cDNA: gi_20466505 Length = 1208 Score = 193 bits (491), Expect(2) = 9e-58 Identities = 94/131 (71%), Positives = 114/131 (86%) Frame = -1 Query: 482 CRALADIIQGRRYSQVCKHLRKIWTTTFRAMDDIKETVRTAGDSLCRAVSSLTIRLCDIS 303 C ALADIIQGR++ QV +HL+K+W FRAMDDIKETVR AGD LCRAV+SLTIR+CD++ Sbjct: 539 CLALADIIQGRKFDQVKEHLKKLWIAAFRAMDDIKETVRNAGDKLCRAVTSLTIRICDVT 598 Query: 302 LTSTSEANETMNIVLPYLLSEGILSKVSSVQKAAISLVMKLAKGAGPALRPHLPELVSCM 123 LT ++A + M+IVLP+LLSEGI+SKV+SV+KA+I +VMKLAKGAG ALRPHL +LV CM Sbjct: 599 LTELADAKQAMDIVLPFLLSEGIMSKVNSVRKASIGVVMKLAKGAGVALRPHLSDLVCCM 658 Query: 122 LECLSSLEDQG 90 LE LSSLEDQG Sbjct: 659 LESLSSLEDQG 669 Score = 47.0 bits (110), Expect(2) = 9e-58 Identities = 20/30 (66%), Positives = 26/30 (86%) Frame = -3 Query: 90 LNYVEMHAGNAGIKTDKLETLRIAVAKDSP 1 LNYVE+HA N GI+T+KLE LRI+++K SP Sbjct: 670 LNYVELHAANIGIETEKLENLRISISKGSP 699 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,310,974 Number of Sequences: 27288 Number of extensions: 166547 Number of successful extensions: 453 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 443 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 453 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 630427000 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18964079|gb|BM660797.1|BM660797 952042A04.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (521 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g25720.1 glutamine cyclotransferase precursor - like protein ... 241 2e-64 >At4g25720.1 glutamine cyclotransferase precursor - like protein / glutamine cyclotransferase precursor, Carica papaya, AF061240;supported by full-length cDNA: Ceres:114197. Length = 320 Score = 241 bits (615), Expect = 2e-64 Identities = 114/173 (65%), Positives = 136/173 (77%) Frame = +1 Query: 1 STRFFSFDLVREYPHDPAAFTQGILYAGNDTLFESTGLYGRSSVRKVELQTGKVLVNHQM 180 S R ++V E+PHDP AFTQG+LYAGNDTLFESTGLYG+SSVRKV+L+TGKV + +M Sbjct: 71 SHRINEIEVVAEFPHDPDAFTQGLLYAGNDTLFESTGLYGKSSVRKVDLRTGKVEILEKM 130 Query: 181 DGDVFGEGLTLLGDRLFQVTWLKNDGFIYDRHNFSKRTSFTHKMRDGWGLATDGKVLFGS 360 D FGEGLTLLG+RLFQV WL N GF YD N SK F H M+DGWGLATDGK LFGS Sbjct: 131 DNTYFGEGLTLLGERLFQVAWLTNTGFTYDLRNLSKVKPFKHHMKDGWGLATDGKALFGS 190 Query: 361 HGTSMLYKLDSKSLEVMKVVTVKYHGNQVPYLNELEYIDGEVWANVWQTDCIA 519 GTS LY++D ++++V V+Y+G +V YLNELEYI+ EVWANVWQ+DCIA Sbjct: 191 DGTSTLYRMDPQTMKVTDKHIVRYNGREVRYLNELEYINNEVWANVWQSDCIA 243 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,311,998 Number of Sequences: 27288 Number of extensions: 281536 Number of successful extensions: 834 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 795 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 832 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 729494100 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18963998|gb|BM660749.1|BM660749 952041E11.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (537 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g22770.1 putative gigantea protein / strong similarity to ... 204 3e-53 >At1g22770.1 putative gigantea protein / strong similarity to GB:CAB56039 from [Arabidopsis thaliana]; supported by cDNA: gi_6002679_gb_AF105064.1_AF105064 Length = 1173 Score = 204 bits (518), Expect = 3e-53 Identities = 103/144 (71%), Positives = 114/144 (78%) Frame = +3 Query: 36 ALACGEILRVLTHYNRPIFKVERQHTEAECXXXXXXXXXXXXXXKRSNNSPGNESDWKPL 215 ALACGEILR+LTHYNRPI+K E+Q+ + E + SP + + KPL Sbjct: 129 ALACGEILRILTHYNRPIYKTEQQNGDTERNCLSKATTSGSPTSEPKAGSP-TQHERKPL 187 Query: 216 RPLTPWITDILLAAPLGIRSDYFRWCGGVMGKYAAGGELKPPTTACSRGSGKHPQLMPST 395 RPL+PWI+DILLAAPLGIRSDYFRWC GVMGKYAAG ELKPPT A SRGSGKHPQLMPST Sbjct: 188 RPLSPWISDILLAAPLGIRSDYFRWCSGVMGKYAAG-ELKPPTIA-SRGSGKHPQLMPST 245 Query: 396 PRWAVANGAGVILSVCDEEVARYE 467 PRWAVANGAGVILSVCD+EVARYE Sbjct: 246 PRWAVANGAGVILSVCDDEVARYE 269 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,394,181 Number of Sequences: 27288 Number of extensions: 217740 Number of successful extensions: 585 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 559 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 582 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 763199020 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18963928|gb|BM660709.1|BM660709 952041B06.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (551 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g38840.1 putative guanylate binding protein / 146 8e-36 At5g46070.1 putative protein / contains similarity to guanyla... 57 5e-09 >At2g38840.1 putative guanylate binding protein / Length = 217 Score = 146 bits (368), Expect = 8e-36 Identities = 66/76 (86%), Positives = 73/76 (95%) Frame = +3 Query: 297 VIGPYRSGKSFLLNQLLSLSCDKGFGVGHMRDTKTKGIWIWGTPVEMDVDGSKVSVLYLD 476 VIGPYRSGKSFLLNQLLSLSC +GFGVGHMRDTKTKGIW+WGTP+E+++DG K SV+YLD Sbjct: 51 VIGPYRSGKSFLLNQLLSLSCYEGFGVGHMRDTKTKGIWVWGTPLELEIDGVKTSVIYLD 110 Query: 477 TEGFESVGKSNVYDDR 524 TEGFESVGKSNVYDDR Sbjct: 111 TEGFESVGKSNVYDDR 126 >At5g46070.1 putative protein / contains similarity to guanylate binding protein Length = 1060 Score = 57.4 bits (137), Expect = 5e-09 Identities = 29/70 (41%), Positives = 44/70 (62%), Gaps = 1/70 (1%) Frame = +3 Query: 288 LTQVIGPYRSGKSFLLNQLLSLSCDKGFGVGHMRDTKTKGIWIWGTPVEMD-VDGSKVSV 464 + V G R GKSF+LNQLL S GF V TKG+W+W +P++ +DG++ ++ Sbjct: 72 VVSVCGRARQGKSFILNQLLGRS--NGFQVASTHKPCTKGLWLWSSPIKRTALDGTEYNL 129 Query: 465 LYLDTEGFES 494 L LD+EG ++ Sbjct: 130 LLLDSEGIDA 139 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,030,001 Number of Sequences: 27288 Number of extensions: 257409 Number of successful extensions: 904 Number of sequences better than 1.0e-05: 4 Number of HSP's better than 0.0 without gapping: 857 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 899 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 808093080 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18963911|gb|BM660700.1|BM660700 952041A10.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (629 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g20050.1 C-8,7 sterol isomerase / identical to GB:AAD03489... 249 8e-67 >At1g20050.1 C-8,7 sterol isomerase / identical to GB:AAD03489 from [Arabidopsis thaliana] (Plant Mol. Biol. 38 (5), 807-815 (1998));supported by full-length cDNA: Ceres:2121. Length = 223 Score = 249 bits (636), Expect = 8e-67 Identities = 114/195 (58%), Positives = 150/195 (76%) Frame = +3 Query: 45 MGHPYAPAELDLPGFVPLKLSQVEILVSYLGASVFVFLAVWLVSGRCVRLSKTDRLLMCW 224 + HPY P +L+LPG+VP+ +S I+ YLG+S+ V VWL+ GR + +K D+LLMCW Sbjct: 4 LAHPYVPRDLNLPGYVPISMSMSSIVSIYLGSSLLVVSLVWLLFGR--KKAKLDKLLMCW 61 Query: 225 WAFTGLTHIMIEGPFVFTPDFFKKENPNFFDEVWKEYSKGDSRYVARDTATVTVEGITAV 404 W FTGLTH+++EG FVF+P+FFK + EVWKEYSKGDSRYV RD+A V+VEGITAV Sbjct: 62 WTFTGLTHVILEGYFVFSPEFFKDNTSAYLAEVWKEYSKGDSRYVGRDSAVVSVEGITAV 121 Query: 405 LEGPASLLVVYAIASRKSFSHILQFAVCLGQLYGCLVYFITAYLDGFNFWVGPFYFWAYF 584 + GPASLL +YAIA KS+S++LQ A+ + QLYGCLVYFITA L+G NF FY+++Y+ Sbjct: 122 IVGPASLLAIYAIAKEKSYSYVLQLAISVCQLYGCLVYFITAILEGDNFATNSFYYYSYY 181 Query: 585 IGANSFWIWIPMLIA 629 IGAN +W+ IP LI+ Sbjct: 182 IGANCWWVLIPSLIS 196 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,463,121 Number of Sequences: 27288 Number of extensions: 241895 Number of successful extensions: 904 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 830 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 903 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 1017362904 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18963872|gb|BM660677.1|BM660677 952040G08.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (454 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g48470.1 expressed protein / ;supported by full-length cD... 211 1e-55 >At5g48470.1 expressed protein / ;supported by full-length cDNA: Ceres:25275. Length = 397 Score = 211 bits (537), Expect = 1e-55 Identities = 103/135 (76%), Positives = 121/135 (89%) Frame = +1 Query: 46 KWDPAECDALLRGGEQVASVLQEMVTLMEDMEMDGAFEQVAVELVAQGVIGKRVDEMESG 225 K DPAE +ALL+GGEQV SVL+EM+TL+EDM+M+ A E VAVEL AQGVIGKRVDEMESG Sbjct: 82 KLDPAEYEALLKGGEQVTSVLEEMITLLEDMKMNEASENVAVELAAQGVIGKRVDEMESG 141 Query: 226 FLMALDYMIQLAQKDTDDERKSILEVIKQTGLDHLTKKCPPHVQVVGLLCQTEKKESRHE 405 F+MALDYMIQLA KD D++RKS+LEV+K+T L HLTKKCPPHVQV+GLLC+T KKESRHE Sbjct: 142 FMMALDYMIQLADKDQDEKRKSLLEVVKETVLSHLTKKCPPHVQVIGLLCRTPKKESRHE 201 Query: 406 SLRRVAAGGGVFKND 450 LRRVAAGGG F+++ Sbjct: 202 LLRRVAAGGGAFESE 216 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.315 0.134 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,596,306 Number of Sequences: 27288 Number of extensions: 171080 Number of successful extensions: 568 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 556 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 568 length of database: 11,516,596 effective HSP length: 90 effective length of database: 9,060,676 effective search space used: 543640560 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18963710|gb|BM660587.1|BM660587 952039G02.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (539 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g63420.1 putative protein / similar to unknown protein (sp... 292 8e-80 >At5g63420.1 putative protein / similar to unknown protein (sp|P54122) Length = 528 Score = 292 bits (747), Expect = 8e-80 Identities = 146/178 (82%), Positives = 167/178 (93%) Frame = +2 Query: 5 DGIIFHTGDWKIDESPVDGKNFDRQALEELSKEGVTLMMSDSTNVLSPGRSISEILVVAG 184 DG I HTGDWKIDE+P+DGK FDR+ALEELSKEGVTLMMSDSTNVLSPGR+ISE VVA Sbjct: 259 DGNILHTGDWKIDEAPLDGKVFDREALEELSKEGVTLMMSDSTNVLSPGRTISE-KVVAD 317 Query: 185 SLLRHISEAKGRVITTQFASNIHRIGSIKAAADLTGRKMVFVGMSLRTYLEAAFKDGKAP 364 +L+R++ AKGRVITTQFASNIHR+GSIKAAAD+TGRK+VFVGMSLRTYLEAA++DGKAP Sbjct: 318 ALVRNVMAAKGRVITTQFASNIHRLGSIKAAADITGRKLVFVGMSLRTYLEAAWRDGKAP 377 Query: 365 LDPSTLIKVEDMDAYAPKDLLVVTTGSQGEPRAALNLASYGGSHALKLSKEDVLLYSA 538 +DPS+LIKVED++AYAPKDLL+VTTGSQ EPRAALNLASYG SHA KL+KED++LYSA Sbjct: 378 IDPSSLIKVEDIEAYAPKDLLIVTTGSQAEPRAALNLASYGSSHAFKLTKEDIILYSA 435 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,706,952 Number of Sequences: 27288 Number of extensions: 287337 Number of successful extensions: 750 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 726 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 748 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 772177832 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18963336|gb|BM660378.1|BM660378 952037C03.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (576 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g05520.1 alpha subunit of F-actin capping protein / suppor... 237 4e-63 >At3g05520.1 alpha subunit of F-actin capping protein / supported by full-length cDNA: Ceres:33531. Length = 308 Score = 237 bits (604), Expect = 4e-63 Identities = 111/184 (60%), Positives = 133/184 (71%) Frame = +1 Query: 25 ELPSAYIEEFRCALDVELSKYVAEAYPKGTCAVYCTSGKDIEGPGADFSFAAVISATKRS 204 ELPS YIEE+R ALD E+ +YV+E+YPKG AV C GKD EGPG+DF +I+A + S Sbjct: 120 ELPSLYIEEYRYALDAEIQRYVSESYPKGMSAVNCVKGKDNEGPGSDFELVVIITAMRLS 179 Query: 205 PQNFCNGSWRSIWTMEFNYELQFVDIKGNIQVDAHYFEEGNVQLDTNIDRKDSTIMQSPD 384 PQNFCNGSWRS+W ++F E Q +DIKG +QV AHYFEEGNV+LD D +DSTI QS D Sbjct: 180 PQNFCNGSWRSVWNIDFQDESQVLDIKGKLQVGAHYFEEGNVELDAKKDFQDSTIFQSAD 239 Query: 385 DCAVSITSIIRHHXXXXXXXXXXXXXXXXDATFKDLRRKLPVTRTLFPWHNTLAVSLTRD 564 DCA++I +IIRHH D TFKDLRRKLPVTRTLFPW NTL SLTR+ Sbjct: 240 DCAIAIANIIRHHETEYLASLEVAYSKLPDNTFKDLRRKLPVTRTLFPWQNTLQFSLTRE 299 Query: 565 LAKQ 576 + K+ Sbjct: 300 VEKE 303 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,792,117 Number of Sequences: 27288 Number of extensions: 227763 Number of successful extensions: 531 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 522 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 531 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 868297828 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18963330|gb|BM660374.1|BM660374 952037B12.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (616 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g80410.1 putative N-terminal acetyltransferase / similar t... 347 2e-96 >At1g80410.1 putative N-terminal acetyltransferase / similar to N-TERMINAL ACETYLTRANSFERASE GB:P12945 from (Saccharomyces cerevisiae); supported by cDNA: gi_17381117 Length = 897 Score = 347 bits (891), Expect = 2e-96 Identities = 165/205 (80%), Positives = 185/205 (89%) Frame = +2 Query: 2 NCMDRKSEAYELVRRGLKNDLKSHVCWHVYGLLYRSDREYREAIKCYRNALRIDPDNIEI 181 NCMDRK+EAYELVR G+KND+KSHVCWHV GLLYRSDREYREAIKCYRNALRIDPDN+EI Sbjct: 54 NCMDRKTEAYELVRLGVKNDIKSHVCWHVLGLLYRSDREYREAIKCYRNALRIDPDNLEI 113 Query: 182 LRDLSLLQAQMRDLSGFVETRQQLLSLKPNHRMNWIGFAVAHHLNSNSSKAVEVLEAYEG 361 LRDLSLLQAQMRDLSGFVETRQQLL+LKPNHRMNWIGFAV+ HLN+N+SKAVE+LEAYEG Sbjct: 114 LRDLSLLQAQMRDLSGFVETRQQLLTLKPNHRMNWIGFAVSQHLNANASKAVEILEAYEG 173 Query: 362 TLEDDYPPENERYEHNEMLLYKISLFEECGMLDRALEEMQKKESKIVDKLSFKEQMASVL 541 TLEDDYPPENE EH EM+LYK+SL EE G D+ALEE+ KKE KIVDKLS+KEQ S+L Sbjct: 174 TLEDDYPPENELIEHTEMILYKVSLLEESGSFDKALEELHKKEPKIVDKLSYKEQEVSLL 233 Query: 542 FKLGRFDESESIYRSLLFMNPDNYK 616 K+GR +E+ +YR LL MNPDNY+ Sbjct: 234 SKVGRLEEANKLYRVLLSMNPDNYR 258 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,034,957 Number of Sequences: 27288 Number of extensions: 234259 Number of successful extensions: 970 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 908 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 966 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 972741724 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18963293|gb|BM660353.1|BM660353 952037A09.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (599 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g74260.1 putative phosphoribosylformylglycinamidine synthase ... 281 2e-76 >At1g74260.1 putative phosphoribosylformylglycinamidine synthase / similar to GB:KIAA0361 from [Homo sapiens] Length = 1387 Score = 281 bits (718), Expect = 2e-76 Identities = 143/199 (71%), Positives = 157/199 (78%) Frame = -3 Query: 597 ASPLQILVDASDGASDYGNKFGEPLIQGYTRTFGMRLLNGERREWLKPIMFSGAIGQIDH 418 ASPLQIL+DAS+GASDYGNKFGEP+IQGYTRTFGMRL +G+RREWLKPIMFS IGQIDH Sbjct: 441 ASPLQILIDASNGASDYGNKFGEPMIQGYTRTFGMRLPSGDRREWLKPIMFSAGIGQIDH 500 Query: 417 AHISKGDPEIGMLVVKIGGPAYRIXXXXXXXXXXXXGQNDAELDFNAVQRGDAEMAQKLY 238 HI+KG+PE+GMLVVKIGGPAYRI GQNDAELDFNAVQRGDAEM+QKLY Sbjct: 501 THITKGEPEVGMLVVKIGGPAYRIGMGGGAASSMVSGQNDAELDFNAVQRGDAEMSQKLY 560 Query: 237 RVVRACAEMGESNPIISIHDQGAGGNCNVVKEIIYPKGAEIDMPFNCCW*SYIVGLRDMG 58 RVVRAC EMGE NPIISIHDQGAGGNCNVVKEIIYP+GAEID+ + L G Sbjct: 561 RVVRACIEMGEKNPIISIHDQGAGGNCNVVKEIIYPQGAEIDIRAVVVGDHTMSVLEIWG 620 Query: 57 C*VPGTGCLLVKLESRSLL 1 +LVK ESR +L Sbjct: 621 AEYQEQDAILVKAESREIL 639 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,413,640 Number of Sequences: 27288 Number of extensions: 293943 Number of successful extensions: 759 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 742 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 758 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 939910020 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18963266|gb|BM660337.1|BM660337 952036H03.x3 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (589 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g42990.1 bZIP family transcription factor / contains Pfam ... 102 2e-22 At3g56660.1 bZIP family transcription factor / similar to Atb... 59 1e-09 At2g40950.1 bZIP family transcription factor / similar to Atb... 55 3e-08 At3g10800.1 bZIP family transcription factor / contains Pfam ... 54 8e-08 At3g17610.1 bZIP family transcription factor / similar to TGA... 50 9e-07 >At1g42990.1 bZIP family transcription factor / contains Pfam profile: PF00170: bZIP transcription factor; supported by cDNA: gi_15028322_gb_AY045964.1_ Length = 295 Score = 102 bits (253), Expect = 2e-22 Identities = 63/136 (46%), Positives = 89/136 (65%), Gaps = 13/136 (9%) Frame = +1 Query: 70 DSGRDD---VLEVVTPEAGTV------EVDGDDPISKKKMRQMRNRDSAMKSRERKKSYI 222 DSG+++ V+E + ++G+ E DD ++KK+ R++RNRD+A++SRERKK Y+ Sbjct: 106 DSGKENSDLVVEKKSNDSGSEIHDDDDEEGDDDAVAKKRRRRVRNRDAAVRSRERKKEYV 165 Query: 223 KDLETKSKHLEAECRRLSYALQCYAAENMALRQSLLKDRPLGAP-TATQESAV-LTETLP 396 +DLE KSK+LE EC RL L+C+ AEN +LR L K + QESAV L E+L Sbjct: 166 QDLEKKSKYLERECLRLGRMLECFVAENQSLRYCLQKGNGNNTTMMSKQESAVLLLESLL 225 Query: 397 LVSLLWL--VSIVCLF 438 L SLLWL V+ +CLF Sbjct: 226 LGSLLWLLGVNFICLF 241 >At3g56660.1 bZIP family transcription factor / similar to AtbZIP transcription factor GI:17065880 from [Arabidopsis thaliana]; contains Pfam profile: PF00170 bZIP transcription factor Length = 620 Score = 59.3 bits (142), Expect = 1e-09 Identities = 30/73 (41%), Positives = 48/73 (65%), Gaps = 1/73 (1%) Frame = +1 Query: 112 AGTVEVDGDDPISKKK-MRQMRNRDSAMKSRERKKSYIKDLETKSKHLEAECRRLSYALQ 288 A ++V G++ KKK +R +RNR+SA SR+RKK Y+++LE K K++ + LS + Sbjct: 160 ASVIKVGGEEDDEKKKNVRLVRNRESAHLSRQRKKHYVEELEDKVKNMHSTISELSSKMS 219 Query: 289 CYAAENMALRQSL 327 + AEN+ LRQ + Sbjct: 220 YFVAENVTLRQQM 232 >At2g40950.1 bZIP family transcription factor / similar to AtbZIP transcription factor GI:17065880 from [Arabidopsis thaliana]; contains Pfam profile: bZIP transcription factor PF00170 Length = 721 Score = 55.1 bits (131), Expect = 3e-08 Identities = 31/86 (36%), Positives = 48/86 (55%) Frame = +1 Query: 70 DSGRDDVLEVVTPEAGTVEVDGDDPISKKKMRQMRNRDSAMKSRERKKSYIKDLETKSKH 249 D R+ +A V G++ KK+ R MRNR+SA SR+RKK Y+++LE K ++ Sbjct: 204 DESRNSKYRRSGEDADASAVTGEED-EKKRARLMRNRESAQLSRQRKKHYVEELEEKVRN 262 Query: 250 LEAECRRLSYALQCYAAENMALRQSL 327 + + L+ + + AEN LRQ L Sbjct: 263 MHSTITDLNGKISYFMAENATLRQQL 288 >At3g10800.1 bZIP family transcription factor / contains Pfam profile: PF00170 bZIP transcription factor; contains similarity to TGACG-sequence specific DNA-binding protein TGA-1B (HSBF) GB:P14233 [Nicotiana tabacum] Length = 675 Score = 53.5 bits (127), Expect = 8e-08 Identities = 30/67 (44%), Positives = 42/67 (61%), Gaps = 1/67 (1%) Frame = +1 Query: 130 DGDDPISKKKM-RQMRNRDSAMKSRERKKSYIKDLETKSKHLEAECRRLSYALQCYAAEN 306 +GDD K+K+ RQ+RNR+SA SR RKK ++LE K K + A L+ + AEN Sbjct: 184 EGDDDDDKRKLIRQIRNRESAQLSRLRKKQQTEELERKVKSMNATIAELNGKIAYVMAEN 243 Query: 307 MALRQSL 327 +ALRQ + Sbjct: 244 VALRQQM 250 >At3g17610.1 bZIP family transcription factor / similar to TGACG-motif binding factor GI:2934884 from [Glycine max]; contains Pfam profile: PF00170 bZIP transcription factor; supported by full-length cDNA: Ceres: 35429. Length = 135 Score = 50.1 bits (118), Expect = 9e-07 Identities = 27/73 (36%), Positives = 42/73 (56%), Gaps = 3/73 (4%) Frame = +1 Query: 133 GDDPISKKKM---RQMRNRDSAMKSRERKKSYIKDLETKSKHLEAECRRLSYALQCYAAE 303 G +P+ K+ R +RNR SA ++RERKK Y+ DLE+++ L+ +L + E Sbjct: 57 GRNPVDKEYRSLKRLLRNRVSAQQARERKKVYVSDLESRANELQNNNDQLEEKISTLTNE 116 Query: 304 NMALRQSLLKDRP 342 N LR+ L+ RP Sbjct: 117 NTMLRKMLINTRP 129 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,207,987 Number of Sequences: 27288 Number of extensions: 235257 Number of successful extensions: 1392 Number of sequences better than 1.0e-05: 10 Number of HSP's better than 0.0 without gapping: 1099 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1329 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 904103924 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18662057|gb|BM501722.1|BM501722 952035B09.x3 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (583 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g20850.1 putative protein / tripeptidyl-peptidase II, Homo... 134 4e-63 >At4g20850.1 putative protein / tripeptidyl-peptidase II, Homo sapiens, Pir:S54376 Length = 1396 Score = 134 bits (337), Expect(2) = 4e-63 Identities = 59/103 (57%), Positives = 75/103 (72%) Frame = +2 Query: 2 ERRFINVPFGASWAEVTMRTSAFDTPRRFFLDTVQICPLKRPVKWEAVVTXXXXXXXXXX 181 ERR+I VP GA+WAE TMRTS FDT RRF++DT+Q+CPL+RP+KWE+ T Sbjct: 799 ERRYIEVPHGATWAEATMRTSGFDTTRRFYIDTLQVCPLRRPIKWESAPTFASPSAKSFV 858 Query: 182 XXVEGGLTLELSIAQFWSSGIASHEPTCVDFEIVLHGISIDQK 310 V G T+EL+IAQFWSSG+ S EPT VDFEI HG+ +D++ Sbjct: 859 FPVVSGQTMELAIAQFWSSGLGSREPTIVDFEIEFHGVGVDKE 901 Score = 124 bits (312), Expect(2) = 4e-63 Identities = 59/87 (67%), Positives = 75/87 (85%), Gaps = 1/87 (1%) Frame = +3 Query: 321 LDG-ESPLLIVARSLLASEKLVPVGTLNKIRIPYRPVECNLSSLPTDRDKLPSGKQIIAL 497 LDG E+P+ + A +LLASEKLVP+ LNKIR+PY+P++ L +L T RD+L SGKQI+AL Sbjct: 905 LDGSEAPIKVEAEALLASEKLVPIAVLNKIRVPYQPIDAQLKTLSTGRDRLLSGKQILAL 964 Query: 498 TLTYKFKLEDNAEIKPHVPLLNNRIYD 578 TLTYKFKLED+AE+KP++PLLNNRIYD Sbjct: 965 TLTYKFKLEDSAEVKPYIPLLNNRIYD 991 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,894,164 Number of Sequences: 27288 Number of extensions: 236895 Number of successful extensions: 534 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 518 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 534 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 886200876 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18661994|gb|BM501687.1|BM501687 952034G11.x2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (546 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g49510.1 von Hippel-Lindau binding protein (VHL binding prote... 281 2e-76 >At5g49510.1 von Hippel-Lindau binding protein (VHL binding protein; VBP) like / ; supported by cDNA: gi_13878182_gb_AF370354.1_AF370354 Length = 195 Score = 281 bits (719), Expect = 2e-76 Identities = 143/179 (79%), Positives = 158/179 (87%), Gaps = 4/179 (2%) Frame = +3 Query: 9 SASTPQG----VAERRGIPAAAFVEDVEAYLRQVGLDVNSALAFLQERLQQYKIVEMKLL 176 S+S+P G + ERRGIPAA F++DVE YL Q GLD NSALAF QERLQQYK+VEMKLL Sbjct: 2 SSSSPSGSGSDLTERRGIPAAKFIQDVETYLSQSGLDPNSALAFHQERLQQYKVVEMKLL 61 Query: 177 AQQRDLQAKIPDIEKCLDIVTTLQAKKDLGEALIADFELSEGIYSRAKIEDTDSVCLWLG 356 AQQRDLQAKIPDIEKCL++V TL+AKK GEAL+ADFE+SEGIYSRA IEDTDSVCLWLG Sbjct: 62 AQQRDLQAKIPDIEKCLEVVATLEAKKGTGEALLADFEVSEGIYSRACIEDTDSVCLWLG 121 Query: 357 ANVMLEYSCDEANALLKKNLENAKASLEVLVADLHFLRDQQTITQVTXARVFNWDVHHR 533 ANVMLEYSC+EA+ALLK NLENAKASLEVLVADL FLRDQ T+TQVT ARV+NWDVH R Sbjct: 122 ANVMLEYSCEEASALLKNNLENAKASLEVLVADLQFLRDQVTVTQVTIARVYNWDVHQR 180 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,528,089 Number of Sequences: 27288 Number of extensions: 216994 Number of successful extensions: 739 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 718 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 738 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 790135456 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18661819|gb|BM501595.1|BM501595 952033C11.x2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (708 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g54440.1 unknown protein / ; supported by cDNA: gi_20259378 338 2e-93 >At5g54440.1 unknown protein / ; supported by cDNA: gi_20259378 Length = 829 Score = 338 bits (866), Expect = 2e-93 Identities = 163/230 (70%), Positives = 193/230 (83%) Frame = +2 Query: 17 SYEKVCALYSAEGWEELLADVLPDLAECQKILNDEAGYLASCVKLLSLDSGLFSSKERQG 196 SYEKVCALY+ EGW++LLA+VLP+LA+CQKIL+D+AGY++SCV+LLSLD GLFSSKERQ Sbjct: 181 SYEKVCALYAGEGWQDLLAEVLPNLAQCQKILDDQAGYMSSCVRLLSLDKGLFSSKERQA 240 Query: 197 FQSEVVRLAHSEMKHLVPLDVSSLITFAGNAGPPLELCDGDPGTLSVAVWNGFPDDITXX 376 FQSEVV LAHSEMK+ VPLDVSSLITF+GN GPPL+LCDGDPG LSV VW+GFPDDIT Sbjct: 241 FQSEVVTLAHSEMKNPVPLDVSSLITFSGNTGPPLQLCDGDPGNLSVTVWSGFPDDITLD 300 Query: 377 XXXXXXXXXXXADEGIKAIKSSDSHVLVPGRNIISFDIPRQKPGSYVLGALTGQIGKLSF 556 DEG +A+KSS + VL PGRN I+F +P QKPGSYVLG +TGQIG+L F Sbjct: 301 SLSLTLVATNNTDEGGQALKSSAATVLNPGRNTITFALPPQKPGSYVLGVVTGQIGRLRF 360 Query: 557 RSHGFSQDGPVETDEFMSFEKPTRPVLKVRKPRALVDITPAVSSALLMNE 706 RSH FS+ GP ++D+FMS+EKPTRP+LKV KPRALVD+ AVSSALL+NE Sbjct: 361 RSHSFSKGGPADSDDFMSYEKPTRPILKVSKPRALVDLAAAVSSALLINE 410 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,694,253 Number of Sequences: 27288 Number of extensions: 303510 Number of successful extensions: 803 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 780 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 803 length of database: 11,516,596 effective HSP length: 96 effective length of database: 8,896,948 effective search space used: 1236675772 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18661770|gb|BM501570.1|BM501570 952033A03.x2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (573 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g58100.1 putative protein / 162 9e-41 >At5g58100.1 putative protein / Length = 945 Score = 162 bits (411), Expect = 9e-41 Identities = 73/107 (68%), Positives = 91/107 (84%) Frame = +1 Query: 250 SSVFSLFNLKDKSKFWTESIIRKEFDDLEGSASRDSSKNGLLNFTRAGNIANYLSLAEVD 429 SSVFSLFNL+DKS+FW+ES+ R +FDDLE S S +G+LN+T++GNIA+YL L EVD Sbjct: 42 SSVFSLFNLRDKSRFWSESVFRTDFDDLESSVH---SNSGVLNYTKSGNIASYLELMEVD 98 Query: 430 SIYLPIPVNFIFIGFDGNGRHEFKLGPEELERWFTKIDHIFEHTRIP 570 S+YLP+PVNFIFIGF+G G +FKL PEELERWF K+DH+FEHTR+P Sbjct: 99 SVYLPVPVNFIFIGFEGKGNQDFKLRPEELERWFNKLDHMFEHTRVP 145 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,926,970 Number of Sequences: 27288 Number of extensions: 214263 Number of successful extensions: 847 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 806 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 845 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 859346304 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18661628|gb|BM501498.1|BM501498 PAC000000001390 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (429 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g10180.1 Deetiolated1 (DET1) light signal transduction protei... 115 1e-26 >At4g10180.1 Deetiolated1 (DET1) light signal transduction protein / Length = 541 Score = 115 bits (287), Expect = 1e-26 Identities = 62/128 (48%), Positives = 89/128 (69%), Gaps = 4/128 (3%) Frame = +1 Query: 1 QNSSEDIYALFEQFYDHFHANPQASSHGKFISSHSNDIHALDQLRVIKNKASSSSQFVKK 180 QNS+ED+Y LFEQF DHF + ++ F++SHSN+++AL+QL+ KNK++S SQFVKK Sbjct: 376 QNSAEDLYQLFEQFSDHFTVS-SSTPFMNFVTSHSNNVYALEQLKYTKNKSNSFSQFVKK 434 Query: 181 MMTSLPYTCQSQSPSPYFDLSLFRYG*EAGFQQLIGIG----IGTEHPIKFMSSGGQNVV 348 M+ SLP++CQSQSPSPYFD SLFR+ ++LI +++PIKF+S + Sbjct: 435 MLLSLPFSCQSQSPSPYFDQSLFRFD-----EKLISAADRHRQSSDNPIKFISRRQPQTL 489 Query: 349 QI*RXKPG 372 + + KPG Sbjct: 490 KF-KIKPG 496 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,287,306 Number of Sequences: 27288 Number of extensions: 195485 Number of successful extensions: 485 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 474 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 484 length of database: 11,516,596 effective HSP length: 89 effective length of database: 9,087,964 effective search space used: 481662092 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18661405|gb|BM501260.1|BM501260 PAC000000000685 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (435 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g60590.1 putative protein / various predicted proteins fro... 112 6e-26 >At5g60590.1 putative protein / various predicted proteins from prokaryotic and eukaryotic species; supported by cDNA: gi_14190378_gb_AF378867.1_AF378867 Length = 164 Score = 112 bits (281), Expect = 6e-26 Identities = 54/69 (78%), Positives = 58/69 (83%) Frame = +1 Query: 4 DTIYGFACDACSAGAVNRIYEIKGRIQTRPLAICVGDVSDISRFALVDHLPHGLLDSLLP 183 DT+YGFACDACS AV+RIYEIKGR T PLAICVGDV DI R A HLPHGLLDSLLP Sbjct: 94 DTLYGFACDACSLEAVSRIYEIKGRKLTSPLAICVGDVLDIKRVATTSHLPHGLLDSLLP 153 Query: 184 GPVTIVLKR 210 GPVT+VL+R Sbjct: 154 GPVTLVLQR 162 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,341,729 Number of Sequences: 27288 Number of extensions: 219011 Number of successful extensions: 569 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 563 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 569 length of database: 11,516,596 effective HSP length: 90 effective length of database: 9,060,676 effective search space used: 489276504 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18661210|gb|BM501290.1|BM501290 PAC000000001019 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (464 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g16440.1 LET1 like protein / 196 5e-51 >At4g16440.1 LET1 like protein / Length = 451 Score = 196 bits (498), Expect = 5e-51 Identities = 97/155 (62%), Positives = 120/155 (76%), Gaps = 6/155 (3%) Frame = +1 Query: 1 LSLIEACNEFVSRYQKNHSCSGQEAGANLPMISSACPGWICYAEKTLGSYILPYISSVKS 180 L LIE+CNEFVSRY++ +S G+ + + LP++SSACPGWICYAEK LGSY+LPY+SSVKS Sbjct: 119 LVLIESCNEFVSRYKQANSDDGENSQSPLPVLSSACPGWICYAEKQLGSYVLPYVSSVKS 178 Query: 181 PQQAIGAAIKHHVVDKLGLKPYDVYHVTVMPCYDKKLEAVRDDFTFSVDGKE------VK 342 PQQAIGAAIKHH+ LGL+ ++VYHVTVMPCYDKKLEA RDDF F DG + + Sbjct: 179 PQQAIGAAIKHHLCQALGLRLHEVYHVTVMPCYDKKLEAARDDFVFD-DGTQDNGDLKLT 237 Query: 343 EVDSVLTTGEVLDLIQSKSTXSRQ*RNPSLDRIVT 447 EVDSVLTTGE++DLI+ K + LDR++T Sbjct: 238 EVDSVLTTGEIMDLIKLKGVDFKDLEESPLDRVLT 272 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,485,135 Number of Sequences: 27288 Number of extensions: 182216 Number of successful extensions: 412 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 407 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 411 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 569103444 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18661200|gb|BM501285.1|BM501285 PAC000000001092 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (390 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g67320.1 expressed protein / contains similarity to DNA pr... 169 4e-43 >At1g67320.1 expressed protein / contains similarity to DNA primase large subunit GI:172234 from [Saccharomyces cerevisiae]; supported by full-length cDNA: Ceres: 116252. Length = 454 Score = 169 bits (428), Expect = 4e-43 Identities = 85/129 (65%), Positives = 109/129 (83%), Gaps = 1/129 (0%) Frame = +3 Query: 3 HFVLRLVYCRTEELRKWFLSMETTLFRYRFRLESPETQRMLMTEFQLPYKALPYSEFEAV 182 HFVLRLVYCR++EL+KWFLSMET LFR+RFRL+ E QR ++ EF LPYKA+ +E E++ Sbjct: 92 HFVLRLVYCRSDELKKWFLSMETALFRHRFRLKKIEEQRAIVGEFGLPYKAVIGAELESL 151 Query: 183 KDKLSQVARSIGQ-SASVEHVFFKVPFEEVPDLVAGRRVFLSKGYAYVAMSQVVSLVVTQ 359 K++L VARS GQ S+ VE++++KVPFEEVPDLVA RRV L KG+A+VA +Q+VSLVVTQ Sbjct: 152 KERLGLVARSHGQISSDVENIYYKVPFEEVPDLVASRRVLLQKGFAFVAGTQLVSLVVTQ 211 Query: 360 FRCNISKAL 386 FR ++SKAL Sbjct: 212 FRSHLSKAL 220 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,410,502 Number of Sequences: 27288 Number of extensions: 166403 Number of successful extensions: 490 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 481 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 489 length of database: 11,516,596 effective HSP length: 88 effective length of database: 9,115,252 effective search space used: 373725332 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18660821|gb|BM501066.1|BM501066 PAC000000000940 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (467 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g12370.1 expressed protein / ; supported by cDNA: gi_14334... 83 6e-17 >At1g12370.1 expressed protein / ; supported by cDNA: gi_14334575_gb_AY034961.1_ Length = 490 Score = 83.2 bits (204), Expect = 6e-17 Identities = 38/79 (48%), Positives = 54/79 (68%), Gaps = 2/79 (2%) Frame = +1 Query: 1 VNKVINEYLVEYPEV-PQWAPWC-MEQPKSVDWDALINSIFSEAENVPEINWCEPGESSA 174 +NK++ +YL+E+P++ P W M K VDWD+LI+ + E VPEI WC PGE + Sbjct: 178 INKLLPDYLIEFPKLEPPKKKWTGMMDKKLVDWDSLIDKVVREGAEVPEIEWCVPGEDAG 237 Query: 175 MEVLLGSKDXVLTKKXKNY 231 +EVL+G+KD LTK+ KNY Sbjct: 238 IEVLMGNKDGFLTKRLKNY 256 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,355,389 Number of Sequences: 27288 Number of extensions: 168269 Number of successful extensions: 463 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 434 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 455 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 578136832 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18660609|gb|BM500954.1|BM500954 PAC000000000805 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (419 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g41010.1 putative protein / contains similarity to metallo... 99 8e-22 At1g53690.1 RNA polymerase II, putative / similar to GI:71718... 70 3e-13 >At5g41010.1 putative protein / contains similarity to metallothionein-I gene transcription activator; supported by cDNA: gi_19310614 Length = 51 Score = 99.0 bits (245), Expect = 8e-22 Identities = 45/52 (86%), Positives = 48/52 (91%) Frame = +1 Query: 70 MDPQQPEPVSYLCGDCGAENTLKPGDVIQCRECGYRILYKKRTRRIVQYEAR 225 MDP PEPV+Y+CGDCG ENTLK GDVIQCRECGYRILYKKRTRR+VQYEAR Sbjct: 1 MDPA-PEPVTYVCGDCGQENTLKSGDVIQCRECGYRILYKKRTRRVVQYEAR 51 >At1g53690.1 RNA polymerase II, putative / similar to GI:717186 from [Homo sapiens] (Mol. Cell. Biol. 15 (9), 4702-4710 (1995)) Length = 61 Score = 70.5 bits (171), Expect = 3e-13 Identities = 32/48 (66%), Positives = 38/48 (78%), Gaps = 1/48 (2%) Frame = +1 Query: 55 LERSTMDPQQPEP-VSYLCGDCGAENTLKPGDVIQCRECGYRILYKKR 195 L++S D +QPE V Y+CGDCG EN LK GDV QCR+CG+RILYKKR Sbjct: 3 LQQSETDDKQPEQLVIYVCGDCGQENILKRGDVFQCRDCGFRILYKKR 50 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,559,101 Number of Sequences: 27288 Number of extensions: 173028 Number of successful extensions: 453 Number of sequences better than 1.0e-05: 4 Number of HSP's better than 0.0 without gapping: 444 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 453 length of database: 11,516,596 effective HSP length: 89 effective length of database: 9,087,964 effective search space used: 454398200 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18660581|gb|BM500939.1|BM500939 PAC000000001329 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (362 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g33410.1 unknown protein / 73 2e-14 >At1g33410.1 unknown protein / Length = 1459 Score = 73.2 bits (178), Expect = 2e-14 Identities = 32/58 (55%), Positives = 43/58 (73%) Frame = +2 Query: 5 ASSRPVDVLHRKKMSAVWXPYTAVERLWCQLGEMQRAGHSVDQCDRLKKLLHGALMSH 178 ASS+P +VL RKK+S VW PYT VERLW +L + +G V+QC +LK+ LH AL++H Sbjct: 1385 ASSKPAEVLKRKKVSGVWFPYTTVERLWWELEKTMNSGRMVEQCHKLKEQLHHALLNH 1442 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,339,639 Number of Sequences: 27288 Number of extensions: 168462 Number of successful extensions: 543 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 525 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 543 length of database: 11,516,596 effective HSP length: 96 effective length of database: 8,896,948 effective search space used: 213526752 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18660419|gb|BM500851.1|BM500851 PAC000000000890 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (424 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g16970.1 expressed protein / ; supported by cDNA: gi_12006... 180 2e-46 >At1g16970.1 expressed protein / ; supported by cDNA: gi_12006423_gb_AF283759.1_AF283759 Length = 621 Score = 180 bits (457), Expect = 2e-46 Identities = 83/132 (62%), Positives = 106/132 (79%) Frame = +2 Query: 14 IEVNTYALVRPTTTGTITWLDSLSNLPLKVERSFICNDTGALLQDXQTRFQMYNDTIVKF 193 IE+N YAL+RP G+ITWLDS +NLP+KVERS+IC DTGA++QD R Q Y + + F Sbjct: 280 IELNGYALLRPAIPGSITWLDSTTNLPVKVERSYICTDTGAIMQDPIQRIQPYKNQNIMF 339 Query: 194 SVRELSEVKRVASHHLRLIGFKPLDCLKDYHNLRPSTFIYPSDERIFGSTCVFXALHSSM 373 +V ELS+VKR+++ HLRL+GFKPL CLKDYHNL+PSTF+YPSD+ + GST F ALH SM Sbjct: 340 TVEELSQVKRISTGHLRLLGFKPLSCLKDYHNLKPSTFLYPSDKEVIGSTRAFIALHRSM 399 Query: 374 LRLGRXALAFYG 409 ++L R A+AFYG Sbjct: 400 IQLERFAVAFYG 411 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,040,695 Number of Sequences: 27288 Number of extensions: 172771 Number of successful extensions: 418 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 413 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 417 length of database: 11,516,596 effective HSP length: 89 effective length of database: 9,087,964 effective search space used: 463486164 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18659892|gb|BM500577.1|BM500577 PAC000000001211 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (461 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g18640.1 3-phosphoserine phosphatase / identical to 3-phos... 218 9e-58 >At1g18640.1 3-phosphoserine phosphatase / identical to 3-phosphoserine phosphatase GI:3759177 from [Arabidopsis thaliana];supported by full-length cDNA: Ceres:34821. Length = 295 Score = 218 bits (556), Expect = 9e-58 Identities = 106/146 (72%), Positives = 123/146 (83%) Frame = +1 Query: 1 LEKRPPRISPGMADLVKKLKSNNIDVFLVSGGFRQMIKPVAFELGIPPENITANQLLFGT 180 L+KRPPR+SPG+ +LVKKL++NNIDV+L+SGGFRQMI PVA LGIP ENI AN LLFG Sbjct: 149 LDKRPPRLSPGIEELVKKLRANNIDVYLISGGFRQMINPVASILGIPRENIFANNLLFGN 208 Query: 181 LGEYARFDPTEPTSRSGGKAKAVQQIKQDHGYKTVVMIGDGATDLEARQPGGADLFICYA 360 GE+ FD EPTSRSGGKAKAVQQI++ YKT+ MIGDGATDLEAR+PGGADLFICYA Sbjct: 209 SGEFLGFDENEPTSRSGGKAKAVQQIRKGRLYKTMAMIGDGATDLEARKPGGADLFICYA 268 Query: 361 GVQMREPVAAQADWVVFDFQELITKL 438 GVQ+RE VAA ADW++F F+ LI L Sbjct: 269 GVQLREAVAANADWLIFKFESLINSL 294 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,253,747 Number of Sequences: 27288 Number of extensions: 250125 Number of successful extensions: 687 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 668 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 687 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 560070056 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18659878|gb|BM500569.1|BM500569 PAC000000001202 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (447 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g41880.1 DNA polymerase alpha subunit IV (primase)-like prote... 185 8e-48 >At5g41880.1 DNA polymerase alpha subunit IV (primase)-like protein / Length = 407 Score = 185 bits (470), Expect = 8e-48 Identities = 86/150 (57%), Positives = 115/150 (76%), Gaps = 1/150 (0%) Frame = +1 Query: 1 HCWVCDSRARKLSNEQRAAIADYFRVYKGGENALKKVSLAGPVLHPFLARSYMDVLKTFF 180 HCWVCD++AR+L+NEQR+A+A+YFRVYKG EN KKV L G LHPFLARSY+D +K+FF Sbjct: 176 HCWVCDAKARRLTNEQRSAVAEYFRVYKGNENNAKKVDLMGHSLHPFLARSYVDFIKSFF 235 Query: 181 EDRLLLNQQLFASEERCRKILDLIPDESVASEVHDKWQGNRRSSISKEDVNATRWEQLKM 360 E L Q +F+S+E+ KIL +I DE + +++ KW+ + RSS+S+E + RWEQLK Sbjct: 236 EGELQATQSIFSSKEKYEKILGMITDEDIQADLRGKWENSARSSLSEEATSLLRWEQLKK 295 Query: 361 TLQSGK-KAQGLRRCVEEIVFSYTYPRLDM 447 LQS + KA LR CVEEIVF++TYPR+D+ Sbjct: 296 ALQSKRNKALSLRTCVEEIVFTFTYPRIDL 325 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,642,899 Number of Sequences: 27288 Number of extensions: 197893 Number of successful extensions: 662 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 649 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 660 length of database: 11,516,596 effective HSP length: 90 effective length of database: 9,060,676 effective search space used: 525519208 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18659719|gb|BM500487.1|BM500487 PAC000000000818 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (394 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g25070.1 putative protein / ; supported by cDNA: gi_20466... 110 3e-25 >At5g25070.1 putative protein / ; supported by cDNA: gi_20466599 Length = 736 Score = 110 bits (274), Expect = 3e-25 Identities = 59/121 (48%), Positives = 84/121 (68%) Frame = +2 Query: 14 IQMQRQKITDARSTLQEVSSRRTSIQQETEALKQKLSFIDKRGPELEAEKKVAAASRNFK 193 +Q +++++ R L+E SS+++ IQQ + K+ FI+KR PELEAEKKVAA++RNFK Sbjct: 526 VQKLQEEVSSTRELLKERSSKKSIIQQNITSFMDKIMFIEKRMPELEAEKKVAASTRNFK 585 Query: 194 EAGRIAAEAKALNSEKDELYGKLEKAATDLETIEKDIVATTNKIQECEGLIELKGRESAL 373 EAGRIAAEAK+LN EKD+ + KA +LE E +I T ++QE E LI K +E A+ Sbjct: 586 EAGRIAAEAKSLNLEKDKTQMETGKANAELEKAEHEIEETIKRLQEIEKLILSKEKELAI 645 Query: 374 T 376 + Sbjct: 646 S 646 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,956,093 Number of Sequences: 27288 Number of extensions: 118305 Number of successful extensions: 572 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 534 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 567 length of database: 11,516,596 effective HSP length: 88 effective length of database: 9,115,252 effective search space used: 382840584 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18659711|gb|BM500483.1|BM500483 PAC000000000820 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (400 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g15770.1 acetyltransferase-like protein / Glucosamine-6-ph... 77 2e-18 >At5g15770.1 acetyltransferase-like protein / Glucosamine-6-phosphate acetyltransferase EMeg32 protein - Mus musculus, EMBL:AJ001006 Length = 149 Score = 77.0 bits (188), Expect(2) = 2e-18 Identities = 38/84 (45%), Positives = 54/84 (64%) Frame = +1 Query: 88 DTIHIRRLECSDHERGFVALLSQLSPCPDLTTSVFATRFAELAAQGDDHIILVAEDPSAS 267 +T IR+LE SD +GF+ LL QL+ +T F RF E+ + GDDH+I V E+ ++ Sbjct: 3 ETFKIRKLEISDKRKGFIELLGQLTVTGSVTDEEFDRRFEEIRSYGDDHVICVIEEETSG 62 Query: 268 DRRILATGCLFVERKFLRGCGKWG 339 +I ATG + +E+KFLR CGK G Sbjct: 63 --KIAATGSVMIEKKFLRNCGKAG 84 Score = 31.2 bits (69), Expect(2) = 2e-18 Identities = 12/23 (52%), Positives = 18/23 (78%) Frame = +3 Query: 330 QVGHVEDVVVDAAARGRGLGLRI 398 + GH+EDVVVD+ RG+ LG ++ Sbjct: 82 KAGHIEDVVVDSRFRGKQLGKKV 104 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,322,349 Number of Sequences: 27288 Number of extensions: 109922 Number of successful extensions: 410 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 402 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 409 length of database: 11,516,596 effective HSP length: 88 effective length of database: 9,115,252 effective search space used: 401071088 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18659611|gb|BM500428.1|BM500428 PAC000000000918 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (461 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g13690.1 alpha-N-acetylglucosaminidase / ; supported by c... 248 1e-66 >At5g13690.1 alpha-N-acetylglucosaminidase / ; supported by cDNA: gi_19423947 Length = 806 Score = 248 bits (632), Expect = 1e-66 Identities = 105/153 (68%), Positives = 129/153 (83%) Frame = -3 Query: 459 GSLPRVPDGGVLIQRPIDWSYSQNAVTSSYSLAWWDWDRWEKEIDWMALQGINLPLAFTG 280 G LPR+ + I+RP+ W+Y QN VTSSYS WW W+RWE+EIDWMALQGINLPLAFTG Sbjct: 138 GHLPRIDSKRIFIRRPVPWNYYQNVVTSSYSYVWWGWERWEREIDWMALQGINLPLAFTG 197 Query: 279 QESIWQRIFQRYNISKSDLDDFFGGPAFLAWSRMANMHGWGGPLPQTWLDDQLVLQKKIL 100 QE+IWQ++F+R+NISK DLDD+FGGPAFLAW+RM N+H WGGPL + WLDDQL+LQK+IL Sbjct: 198 QEAIWQKVFKRFNISKEDLDDYFGGPAFLAWARMGNLHAWGGPLSKNWLDDQLLLQKQIL 257 Query: 99 SRMYSFGMFPVLPAFSGNIPAALKSKFPSAKVT 1 SRM FGM PVLP+FSGN+P+AL+ +P A +T Sbjct: 258 SRMLKFGMTPVLPSFSGNVPSALRKIYPEANIT 290 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,948,633 Number of Sequences: 27288 Number of extensions: 251849 Number of successful extensions: 764 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 710 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 764 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 560070056 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18659594|gb|BM500419.1|BM500419 PAC000000000906 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (457 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g72050.1 C2H2-type zinc finger protein, putative / contain... 86 7e-18 At3g48430.1 similar to zinc finger protein - like / temporary... 49 1e-06 >At1g72050.1 C2H2-type zinc finger protein, putative / contains multiple zinc finger domains: PF00096: Zinc finger, C2H2 type; supported by cDNA: gi_15809914_gb_AY054225.1_ Length = 324 Score = 86.3 bits (212), Expect = 7e-18 Identities = 52/169 (30%), Positives = 76/169 (44%), Gaps = 55/169 (32%) Frame = +1 Query: 94 KRPFSCTVDGCPFSYSRKDHLNRHLLTHEGKLFVCPVEGCGRKFNIKGNMQRHVQEIHK- 270 +R F+C VD C SY RKDHLNRHLLTH+GKLF CP E C +F+++GN+ RHV++ H Sbjct: 3 ERSFTCYVDDCAASYRRKDHLNRHLLTHKGKLFKCPKENCKSEFSVQGNVGRHVKKYHSN 62 Query: 271 ---------------------------------------------------DVPPCE--- 288 D P E Sbjct: 63 DNRDKDNTGLGDGDKDNTCKGDDDKEKSGSGGCEKENEGNGGSGKDNNGNGDSQPAECST 122 Query: 289 SKKEFICPKVNCGEGFQICF*VKEA*RITCXAGNTPEVMCCEPGCMKSF 435 +K+ +C ++ CG+ F+ +++ + + ++ E C EPGCMK F Sbjct: 123 GQKQVVCKEIGCGKAFKYPSQLQKH-QDSHVKLDSVEAFCSEPGCMKYF 170 Score = 46.2 bits (108), Expect = 8e-06 Identities = 24/69 (34%), Positives = 37/69 (52%), Gaps = 5/69 (7%) Frame = +1 Query: 145 KDHLNRHLLTHE-----GKLFVCPVEGCGRKFNIKGNMQRHVQEIHKDVPPCESKKEFIC 309 K ++ RHL TH+ G++ C VEGC F+ N+Q+H++ +H D+ P F+C Sbjct: 198 KKNIKRHLRTHDEDSSPGEI-KCEVEGCSSTFSKASNLQKHMKAVHDDIRP------FVC 250 Query: 310 PKVNCGEGF 336 CG F Sbjct: 251 GFPGCGMRF 259 >At3g48430.1 similar to zinc finger protein - like / temporary automated functional assignment Length = 1354 Score = 48.9 bits (115), Expect = 1e-06 Identities = 33/112 (29%), Positives = 51/112 (45%) Frame = +1 Query: 103 FSCTVDGCPFSYSRKDHLNRHLLTHEGKLFVCPVEGCGRKFNIKGNMQRHVQEIHKDVPP 282 + C ++GC S+S + L H K +CP++GCG+ F + +H Q +H D P Sbjct: 1237 YQCNMEGCTMSFSSEKQLMLH------KRNICPIKGCGKNFFSHKYLVQH-QRVHSDDRP 1289 Query: 283 CESKKEFICPKVNCGEGFQICF*VKEA*RITCXAGNTPEVMCCEPGCMKSFQ 438 + CP C F+ + E I G P V C EP C ++F+ Sbjct: 1290 LK------CPWKGCKMTFKWAWSRTE--HIRVHTGARPYV-CAEPDCGQTFR 1332 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,242,256 Number of Sequences: 27288 Number of extensions: 214017 Number of successful extensions: 472 Number of sequences better than 1.0e-05: 4 Number of HSP's better than 0.0 without gapping: 441 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 467 length of database: 11,516,596 effective HSP length: 90 effective length of database: 9,060,676 effective search space used: 552701236 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18659504|gb|BM500373.1|BM500373 PAC000000000489 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (457 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g48850.1 chorismate synthase, putative / similar to choris... 205 1e-58 >At1g48850.1 chorismate synthase, putative / similar to chorismate synthase GI:452796 from [Synechocystis sp.]; supported by cDNA: gi_15982823_gb_AY057519.1_ Length = 436 Score = 205 bits (521), Expect(2) = 1e-58 Identities = 98/125 (78%), Positives = 109/125 (86%) Frame = +3 Query: 6 IESNIVRCPDPEYAEKMIAAIDTVRVRGDSIGGVVTCIARNVPRGLGSPVFDKLEAELAK 185 IE+NIVRCP+PEYAEKMIAAID VR +G+S+GGVVTCI RN PRGLG+PVFDKLEAELAK Sbjct: 229 IENNIVRCPNPEYAEKMIAAIDAVRTKGNSVGGVVTCIVRNAPRGLGTPVFDKLEAELAK 288 Query: 186 AMLSLPASKGFEIGSGFAGTDFTGSEHNDEFYMDEAGNVRTRTNRSGGVQGGISNGEIIY 365 A +SLPA+KGFE GSGFAGT TG EHNDEFY DE G +RTRTNRSGG+QGGISNGEII Sbjct: 289 ACMSLPATKGFEFGSGFAGTFLTGLEHNDEFYTDENGRIRTRTNRSGGIQGGISNGEIIN 348 Query: 366 FQSGF 380 + F Sbjct: 349 MRVAF 353 Score = 38.1 bits (87), Expect(2) = 1e-58 Identities = 16/34 (47%), Positives = 25/34 (73%) Frame = +1 Query: 355 KLFTSKVAFKPTATIXKKPNTVSREHEDVELXAR 456 ++ +VAFKPT+TI +K NTV+R+ + E+ AR Sbjct: 345 EIINMRVAFKPTSTIGRKQNTVTRDKVETEMIAR 378 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,045,449 Number of Sequences: 27288 Number of extensions: 217075 Number of successful extensions: 628 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 591 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 627 length of database: 11,516,596 effective HSP length: 90 effective length of database: 9,060,676 effective search space used: 552701236 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18659090|gb|BM500149.1|BM500149 PAC000000000269 Pioneer AF-1 array Zea mays cDNA, mRNA sequence (423 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g12200.1 dihydropyrimidinase / ; supported by cDNA: gi_13... 163 3e-41 >At5g12200.1 dihydropyrimidinase / ; supported by cDNA: gi_13878036_gb_AF370281.1_AF370281 Length = 248 Score = 163 bits (413), Expect = 3e-41 Identities = 77/90 (85%), Positives = 83/90 (91%) Frame = +1 Query: 154 MSIDAMDEIAKAKREGQRVIGEPVVSGLVLDDSWLWDPDFTIASKYVMSPPIREAGHGKA 333 MS+DAMDEIAKA++ GQ+VIGEPVVSGL+LDD WLWDPDFTIASKYVMSPPIR GHGKA Sbjct: 1 MSVDAMDEIAKARKSGQKVIGEPVVSGLILDDHWLWDPDFTIASKYVMSPPIRPVGHGKA 60 Query: 334 LQAALSSGILQLVGTDHCTFNSTQKAFGSD 423 LQ ALS+GILQLVGTDHCTFNSTQKA G D Sbjct: 61 LQDALSTGILQLVGTDHCTFNSTQKALGLD 90 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,790,435 Number of Sequences: 27288 Number of extensions: 169012 Number of successful extensions: 411 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 409 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 411 length of database: 11,516,596 effective HSP length: 89 effective length of database: 9,087,964 effective search space used: 463486164 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18655245|gb|BM500050.1|BM500050 952036D04.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (501 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g09180.1 expressed protein / ; supported by full-length cD... 217 3e-57 >At3g09180.1 expressed protein / ; supported by full-length cDNA: Ceres: 119803. Length = 402 Score = 217 bits (552), Expect = 3e-57 Identities = 108/149 (72%), Positives = 125/149 (83%) Frame = +1 Query: 52 DAPPKQVAQAMERLGRAGRLIADIRLGADRLLEALFVSGDAPPYSVRQHVDRMEHIIXKE 231 DAPPKQVAQAMERL +A R+IADIRLGADR+LEA+FV+ S +H D + +E Sbjct: 24 DAPPKQVAQAMERLNQAARVIADIRLGADRILEAMFVA------SQPRHTDMPLQLFLRE 77 Query: 232 DAAMRLHFQDLRALGRQLEESGVLNGALKARGNLWGLHMPLVCPDGAVVAYAWKRQLAGQ 411 DA+MR H QDLR +G++LEESGVL +L++R N WGLHMPLVCPDGAVVAYAWKRQLAGQ Sbjct: 78 DASMRQHLQDLRLIGKKLEESGVLTESLRSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQ 137 Query: 412 AGASAVDRTRLALKAFTDQKRRFFPHLED 498 AGASAVDRTRLALKAFTDQKRRFFPH++D Sbjct: 138 AGASAVDRTRLALKAFTDQKRRFFPHIDD 166 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,319,005 Number of Sequences: 27288 Number of extensions: 148405 Number of successful extensions: 408 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 400 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 407 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 666451400 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18655202|gb|BM500007.1|BM500007 952036A04.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (519 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g16860.1 Expressed protein / ; supported by full-length cD... 186 8e-48 >At2g16860.1 Expressed protein / ; supported by full-length cDNA: Ceres: 34622. ; supported by cDNA: gi_20466269 Length = 298 Score = 186 bits (471), Expect = 8e-48 Identities = 91/144 (63%), Positives = 107/144 (74%) Frame = -1 Query: 519 ELRLKMNEARKASQQAMVAEKKRMEPRGESRGVSKQKWLADRKKKIGKLLDSNGLDMKMA 340 ELRLKMNEARK++Q + +EKK+ME E++G+SKQKWL RKKKIGKLLD+NGLDM A Sbjct: 92 ELRLKMNEARKSNQTDVGSEKKKMEAPTETKGISKQKWLEGRKKKIGKLLDANGLDMTQA 151 Query: 339 YMLDTQXXXXXXXXXXXXXXXXYGWDVFNQKTLYDAYKKRTKNIEVDMESYNKAKEADPE 160 YMLDTQ GWDVFNQKTLY+AYKKRTKNI+VD+E YN+ + ADPE Sbjct: 152 YMLDTQEAAESKYKKWEKEPTPAGWDVFNQKTLYNAYKKRTKNIQVDLEEYNRMRAADPE 211 Query: 159 FYRNASSLQYGKVSNVPEENIDKM 88 FYR ASSLQYGK ++ IDKM Sbjct: 212 FYREASSLQYGKAPKTSQDKIDKM 235 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,735,731 Number of Sequences: 27288 Number of extensions: 190486 Number of successful extensions: 554 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 547 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 553 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 720488000 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18655109|gb|BM499914.1|BM499914 952035E06.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (532 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g20410.1 expressed protein / ;supported by full-length cDN... 253 2e-69 >At2g20410.1 expressed protein / ;supported by full-length cDNA: Ceres:13277. Length = 339 Score = 253 bits (646), Expect(2) = 2e-69 Identities = 114/153 (74%), Positives = 131/153 (85%) Frame = +1 Query: 73 HGIKRVEGRSWPSPITGRLWIHAASKVPDPDTIKAMEDFYREIYAVDGITGIKFPDHYPV 252 HGIKR+EGRSWPSPI GRLWIHAASKVPD TIKAME+FY++IYAVDGIT I+FP HYPV Sbjct: 23 HGIKRIEGRSWPSPIRGRLWIHAASKVPDEATIKAMEEFYQQIYAVDGITDIQFPQHYPV 82 Query: 253 SRLLGCVEVVGCLRSEELVCWEHVPESVRLEGLTDFXWLCENPQKLVVPFEMRGYQGVYN 432 SRL+GCVEVVGC+ S+EL W+ +P+ VRLEG T+F WLCE PQKL++PFEMRGYQGVYN Sbjct: 83 SRLIGCVEVVGCVTSDELQNWDALPQGVRLEGQTNFCWLCEKPQKLIIPFEMRGYQGVYN 142 Query: 433 LERRIYDGAVRGLLPVQGPLPVKFPLPDPRDPF 531 LE +IY A RGL+P Q VKFPLPDP+DPF Sbjct: 143 LENKIYVAAARGLMPSQNSFKVKFPLPDPKDPF 175 Score = 26.6 bits (57), Expect(2) = 2e-69 Identities = 10/16 (62%), Positives = 12/16 (74%) Frame = +3 Query: 9 LSAGIPREPCLTMHQP 56 +S G + PCLTMHQP Sbjct: 1 MSRGNYKNPCLTMHQP 16 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,945,003 Number of Sequences: 27288 Number of extensions: 304221 Number of successful extensions: 791 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 759 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 789 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 745241396 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18654958|gb|BM499763.1|BM499763 952034F08.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (561 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g65540.1 unknown protein / 94 6e-20 >At5g65540.1 unknown protein / Length = 595 Score = 93.6 bits (231), Expect = 6e-20 Identities = 63/165 (38%), Positives = 86/165 (51%), Gaps = 5/165 (3%) Frame = -2 Query: 557 VSIDVLSEIIIKPICKLGRNLKLLTDSYRKQFSSIELLKMFLQTVGYSNIGSLMEITKMG 378 V IDV S+++ + + KLGR LKLLTDSY+K+ S+++L+KMFL T GYSN+GSL EI K G Sbjct: 350 VPIDVFSQLVSRHMSKLGRILKLLTDSYKKECSAMQLIKMFLNTTGYSNLGSLAEIVKDG 409 Query: 377 NRVANYPIHQDAQVLQTQNAISIHA-----QQLPRQFPPQMXXXXXXXXXXXXXXXXXXX 213 R P + QVLQ Q + A QQ+ RQ PQM Sbjct: 410 TRNHPPPNQKQPQVLQQQLHLQQQASLRLPQQIQRQMHPQMQQMVNPQNFQQQQQLERMR 469 Query: 212 XXXXXXXXXXXXXXLCSPRGPLTMADKNQPMVNVKVENTMDSQID 78 SPR + M +K++P+V VK+EN + +D Sbjct: 470 RRPVT-----------SPRPNMDM-EKDRPLVQVKLENPSEMAVD 502 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,281,196 Number of Sequences: 27288 Number of extensions: 154196 Number of successful extensions: 351 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 344 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 351 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 835029516 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18654809|gb|BM499614.1|BM499614 952033C09.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (562 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g16300.1 low density lipoprotein B-like protein / 168 2e-42 >At5g16300.1 low density lipoprotein B-like protein / Length = 1068 Score = 168 bits (426), Expect = 2e-42 Identities = 82/165 (49%), Positives = 114/165 (68%) Frame = -2 Query: 561 GGGFWFSESKIKKGGVLAHLKPIADENDFHSCLTLYFGPEVSRIRSAIDSKCKNILDDLL 382 GGG WF E KK G+++ K +E+DF SCLT YFGPEVS++R A+D +C ++L+DLL Sbjct: 485 GGGVWFIEPNSKKVGLISGNKSSPEESDFQSCLTAYFGPEVSQMRDAVDRRCHSVLEDLL 544 Query: 381 SFVESHNSAPRLKELVPYLQEKCYRTISGVLKELEAELRKLSALLGTKKEGNDIPAASII 202 SF ES + PRLK+L PY+Q KCY ++S +L +++ EL L A + + + ++ +II Sbjct: 545 SFFESEKAGPRLKDLAPYVQNKCYDSVSALLADVDKELEFLCAAVKKENKDSEAIPPAII 604 Query: 201 AERSLFIGRILFALRYYSSHVPLILGSPREWVEEAGGAAFARLSS 67 E+SLF+GR+LFAL +S HVPLILGSPR W E A +LSS Sbjct: 605 IEKSLFMGRLLFALLNHSKHVPLILGSPRLWCRETMTAVSDKLSS 649 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,899,654 Number of Sequences: 27288 Number of extensions: 287712 Number of successful extensions: 871 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 848 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 871 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 835029516 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18650404|gb|BM499223.1|BM499223 947043H10.x1 947 - 2 week shoot from Barkan lab Zea mays cDNA, mRNA sequence (540 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g52120.1 gamma response I protein / ; supported by cDNA: ... 138 2e-33 >At3g52120.1 gamma response I protein / ; supported by cDNA: gi_14335159_gb_AY037259.1_ Length = 443 Score = 138 bits (347), Expect = 2e-33 Identities = 65/105 (61%), Positives = 73/105 (68%) Frame = -2 Query: 539 RCNDXXXXXXXXXXXXKVKIQADNIGHKLLSKMGWXXXXXXXXXXXXRADPVMAGDVKKD 360 +CND K KIQADN+GHKLLSKMGW ADP+MAGDVK + Sbjct: 339 KCNDAAAQKATKEAAEKAKIQADNVGHKLLSKMGWKEGEGIGSSRKGMADPIMAGDVKTN 398 Query: 359 HLGVGAVQPGEVTSEDDIYEQYKKRMMLGYRYRPNPLNNPRKSYY 225 +LGVGA PGEV EDDIYEQYKKRMMLGY++RPNPL NPRK+YY Sbjct: 399 NLGVGASAPGEVKPEDDIYEQYKKRMMLGYKHRPNPLGNPRKAYY 443 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,606,068 Number of Sequences: 27288 Number of extensions: 257761 Number of successful extensions: 664 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 646 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 664 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 772177832 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18650345|gb|BM499164.1|BM499164 947043A12.x1 947 - 2 week shoot from Barkan lab Zea mays cDNA, mRNA sequence (616 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g22660.1 putative tRNA adenylyltransferase / Similar to tR... 130 7e-31 >At1g22660.1 putative tRNA adenylyltransferase / Similar to tRNA adenylyltransferase gb|U15930 from Lupinus albus. EST gb|AA721797 comes from this gene; supported by cDNA: gi_13877576_gb_AF370489.1_AF370489 Length = 605 Score = 130 bits (326), Expect = 7e-31 Identities = 62/139 (44%), Positives = 93/139 (66%), Gaps = 6/139 (4%) Frame = -3 Query: 611 IPGDSVKRVFAGLILREIKEFWRVALLLSILFYRETGTPAGTLDEQD------ELRRRKE 450 IP S RV G +LR+IK+FWRV+LL S+L + T G+ D QD +L R +E Sbjct: 470 IPATSKIRVLTGFLLRDIKDFWRVSLLTSLLL---SATVDGSNDHQDIGQLDFQLERMRE 526 Query: 449 KYIRAERSITDLDLDGVWKLKPILDGKSIMGVMQVKSGGPLIGKWQQRVLKWQLAHPDGT 270 Y+ E +I +L LD +W KP+++G+ IM + ++K G LI +WQQ++L WQLA+P+GT Sbjct: 527 TYLTVEATIHELGLDKIWDAKPLVNGREIMQIAELKGGSRLIREWQQKLLTWQLAYPNGT 586 Query: 269 VDECVEWMKQSQSKRQKVE 213 +EC EWM+ ++KRQ++E Sbjct: 587 AEECKEWMRDIKAKRQRIE 605 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,793,021 Number of Sequences: 27288 Number of extensions: 272769 Number of successful extensions: 796 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 766 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 795 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 972741724 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18650250|gb|BM499069.1|BM499069 949015G11.x1 949 - Juvenile leaf and shoot cDNA from Steve Moose Zea mays cDNA, mRNA sequence (428 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g12340.2 FKBP-type peptidyl-prolyl cis-trans isomerase, putat... 64 3e-11 >At3g12340.2 FKBP-type peptidyl-prolyl cis-trans isomerase, putative / contains Pfam profile: PF00254, FKBP-type peptidyl-prolyl cis-trans isomerases Length = 186 Score = 63.9 bits (154), Expect = 3e-11 Identities = 34/88 (38%), Positives = 51/88 (57%) Frame = -3 Query: 330 VTLPPVPLREPRSALGAPVELGFTAYSERLIXXXXXXXXXXXXXVELGSGQALVKNNQPA 151 + LPPV L+E S G VE GF+ +SER+ VEL +G++++ + P+ Sbjct: 98 IYLPPVSLKEAVSG-GLKVEPGFSPFSERINGRIAGLGLTALLLVELATGKSVLNYHTPS 156 Query: 150 TLFLQAYTVXXXXALFIKYEKERISTWP 67 +FLQ Y V A+F+K EKE++S WP Sbjct: 157 VVFLQIYFVAAVSAMFVKIEKEKVSVWP 184 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,781,571 Number of Sequences: 27288 Number of extensions: 67412 Number of successful extensions: 176 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 174 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 176 length of database: 11,516,596 effective HSP length: 89 effective length of database: 9,087,964 effective search space used: 481662092 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18649854|gb|BM498673.1|BM498673 952019G08.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (546 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g18380.1 unknown protein / ; supported by cDNA: gi_20260285 191 2e-49 >At3g18380.1 unknown protein / ; supported by cDNA: gi_20260285 Length = 348 Score = 191 bits (485), Expect = 2e-49 Identities = 94/127 (74%), Positives = 103/127 (81%) Frame = -3 Query: 421 YDVAAFLSHRLFESGDPEVRVRFSGFGAEEDEWINFRKCVRQRSLPCEATECVAVLPGDL 242 YDV AFL+HR E GDPEV+VRF+GF EEDEWIN +K VRQRSLPCEA+ECVAVL GDL Sbjct: 156 YDVQAFLAHRNLEIGDPEVQVRFAGFEVEEDEWINVKKHVRQRSLPCEASECVAVLAGDL 215 Query: 241 ILCFQEGKDQALYYDAHVLDAQXXXXXXXXXXXRFLVRYDHDSSEEIVPLRKVCRRPETD 62 +LCFQEGKDQALY+DA VLDAQ RFLVRY HD SEEIVPLRK+CRRPETD Sbjct: 216 VLCFQEGKDQALYFDAIVLDAQRRRHDVRGCRCRFLVRYSHDQSEEIVPLRKICRRPETD 275 Query: 61 YRLQKNH 41 YRLQ+ H Sbjct: 276 YRLQQLH 282 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,074,657 Number of Sequences: 27288 Number of extensions: 267357 Number of successful extensions: 935 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 858 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 934 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 790135456 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18649795|gb|BM498614.1|BM498614 952019C12.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (616 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g13235.1 Expressed protein / ; supported by full-length cD... 249 6e-67 >At3g13235.1 Expressed protein / ; supported by full-length cDNA: Ceres: 12734. Length = 414 Score = 249 bits (637), Expect = 6e-67 Identities = 133/199 (66%), Positives = 145/199 (72%) Frame = -2 Query: 615 PEAFARVVMLYVDMEVNGVPLKAFVDSGAQSTIISKSCAERCGXXXXXXXXXRGIAVGVG 436 PE FARV+MLYVDMEVNGVPLKAFVDSGAQSTIISKSCAERCG +GIA GVG Sbjct: 193 PEGFARVIMLYVDMEVNGVPLKAFVDSGAQSTIISKSCAERCGLLRLMDQRYKGIAHGVG 252 Query: 435 QSEILGRIHVAPIKIGNIFYPCSFTVLDAPNMEFLFGLDMLRKHQCMIDLKDNVLRVGGG 256 Q+EILGRIHVAPIKIGN FYPCSF VLD+PNMEFLFGLDMLRKHQC IDLK+NV+ VGGG Sbjct: 253 QTEILGRIHVAPIKIGNNFYPCSFVVLDSPNMEFLFGLDMLRKHQCTIDLKENVMTVGGG 312 Query: 255 EVSVPFLQEKDIPAHIHNEEKLSKLGSLSQATGESSRTREXXXXXXXXXXXXXXXXXXXX 76 EVSVPFLQEKDIP+ +EE++ S S AT S T E Sbjct: 313 EVSVPFLQEKDIPSRFLDEERVPNDASSSGATVPSGFT-EKKNNTVANPTSQQPKRQNTS 371 Query: 75 XXGDFEAKVTKLVELGFDR 19 +FEAK+ KLVELGF R Sbjct: 372 EGPEFEAKIAKLVELGFSR 390 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,489,581 Number of Sequences: 27288 Number of extensions: 243929 Number of successful extensions: 665 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 646 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 664 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 972741724 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18649776|gb|BM498595.1|BM498595 952019A07.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (369 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g40740.1 putative protein / ; supported by cDNA: gi_14532... 102 2e-24 >At5g40740.1 putative protein / ; supported by cDNA: gi_14532693_gb_AY039971.1_ Length = 741 Score = 102 bits (254), Expect(2) = 2e-24 Identities = 48/71 (67%), Positives = 58/71 (81%) Frame = -3 Query: 259 QEKEEALTRSDDRNGRTQQTVDVAEILRRWTHALQRIHKQSLHLAKANDGEGPELLRSAS 80 Q +E L+R DDR G+ QTVDVAE++RRWTHALQRIHKQSL LAKANDG+GP++LR+AS Sbjct: 351 QASDETLSRVDDRGGKINQTVDVAEVIRRWTHALQRIHKQSLLLAKANDGDGPDILRTAS 410 Query: 79 SGEASTHSDSL 47 G S H +SL Sbjct: 411 DGGTSGHVESL 421 Score = 25.4 bits (54), Expect(2) = 2e-24 Identities = 10/16 (62%), Positives = 12/16 (74%) Frame = -2 Query: 50 VTATLAEHRQHLVSIQ 3 + ATL EH+QHL S Q Sbjct: 421 LAATLTEHQQHLASFQ 436 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,077,093 Number of Sequences: 27288 Number of extensions: 128938 Number of successful extensions: 394 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 382 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 393 length of database: 11,516,596 effective HSP length: 87 effective length of database: 9,142,540 effective search space used: 319988900 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18649725|gb|BM498544.1|BM498544 952032C11.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (627 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g65260.1 expressed protein / contains similarity to chloro... 243 4e-65 >At1g65260.1 expressed protein / contains similarity to chloroplast membrane-associated protein IM30 GI:169106 from [Pisum sativum];supported by full-length cDNA: Ceres:32284. Length = 330 Score = 243 bits (621), Expect = 4e-65 Identities = 129/181 (71%), Positives = 153/181 (84%), Gaps = 3/181 (1%) Frame = +3 Query: 93 SFRRTPLRTSFL-RGSVSLKVVQVRQSNVNRFKCNS--IRSNLFDRLTRVVRSYANAVLS 263 S R PLRTSF S +L+V +R + NR +CN NLF+R +RVV+SYANA++S Sbjct: 32 SLRILPLRTSFFGNSSGALRVNVLRLACDNRLRCNGHGATMNLFERFSRVVKSYANALIS 91 Query: 264 SFEDPEKILDQAVLEMNDDLTKMRQATAQVLASQKRLENKYKAAEQASADWYRRAQLALQ 443 SFEDPEKIL+Q V+EMN DLTKMRQATAQVLASQK+L+NKYKAA+Q+S DWY+RAQLAL Sbjct: 92 SFEDPEKILEQTVIEMNSDLTKMRQATAQVLASQKQLQNKYKAAQQSSDDWYKRAQLALA 151 Query: 444 KGDEDLAREALKRRKSYADNASSLRSQLDQQKGVVENLISNTRLLESKISEAKQKKDTLK 623 KGDEDLAREALKRRKS+ADNA++L++QLDQQKGVV+NL+SNTRLLESKI EAK KKDTL Sbjct: 152 KGDEDLAREALKRRKSFADNATALKTQLDQQKGVVDNLVSNTRLLESKIQEAKAKKDTLL 211 Query: 624 A 626 A Sbjct: 212 A 212 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,386,327 Number of Sequences: 27288 Number of extensions: 186980 Number of successful extensions: 899 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 769 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 885 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 1008438668 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18649611|gb|BM498430.1|BM498430 952026C01.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (533 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g05760.1 expressed protein / ; supported by cDNA: gi_14517... 161 6e-42 >At3g05760.1 expressed protein / ; supported by cDNA: gi_14517382_gb_AY039526.1_ Length = 202 Score = 161 bits (407), Expect(2) = 6e-42 Identities = 75/104 (72%), Positives = 88/104 (84%) Frame = -1 Query: 353 SGNPVGVDNTSRRKFXKEEYLERARQREQREKDEARKGKERGPPVQRQPLKHRDYEVDLD 174 S GVDNT R+KF EE+ ERAR+RE++E D + K + +GPPVQR PLKHRDY VDL+ Sbjct: 4 SNTTTGVDNTFRKKFDVEEFKERAREREKKESDRS-KSRSKGPPVQRAPLKHRDYHVDLE 62 Query: 173 SRLGKTQVVTPIAPLNQQAGYYCSVCECVVKDSANYLDHINGKK 42 SRLGKTQVVTP+APL+QQAGY+C VC+CVVKDSANYLDHINGKK Sbjct: 63 SRLGKTQVVTPVAPLSQQAGYFCRVCDCVVKDSANYLDHINGKK 106 Score = 26.6 bits (57), Expect(2) = 6e-42 Identities = 12/13 (92%), Positives = 13/13 (99%) Frame = -2 Query: 40 RALGMSMRVERAS 2 RALGMSMRVER+S Sbjct: 109 RALGMSMRVERSS 121 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,281,466 Number of Sequences: 27288 Number of extensions: 236853 Number of successful extensions: 1091 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 964 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1058 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 754220208 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18649562|gb|BM498381.1|BM498381 952024C01.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (573 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g35400.1 putative protein / 150 8e-40 >At5g35400.1 putative protein / Length = 411 Score = 150 bits (379), Expect(2) = 8e-40 Identities = 77/159 (48%), Positives = 99/159 (61%), Gaps = 2/159 (1%) Frame = -2 Query: 500 HVHYKHTDACHQLRWTAKESYEYMYARPWSRVVDFYAELVRXXXXXXXXAKLFGNDEKDY 321 +VHY H DAC + RW A+E++ +MY RPW V+DFY+ V + K Sbjct: 6 YVHYHHADACSKARWNARETFRFMYDRPWQHVLDFYSNAVEGNLSVLSLFQ-----PKKS 60 Query: 320 TLYTAG--ENYLTPSEKPTTTTFSKGRGGRWERVTFKVVISYHGGSFDGWQKQPGLNTVQ 147 ++ G E L SE P T+T GR GRWERV FK+V+SY+G SFDGWQKQP L+TVQ Sbjct: 61 LVHDDGITEEMLLESE-PETSTRKDGRSGRWERVNFKIVLSYNGASFDGWQKQPDLHTVQ 119 Query: 146 GLVEKHLGQFVDERKAKQLEARSLPIEGCALMLDALTKG 30 G+VEK LG FVDERKA+ L+ + P+EG L+ KG Sbjct: 120 GVVEKSLGGFVDERKAQLLKKKCKPLEGRVLVAGRTDKG 158 Score = 30.4 bits (67), Expect(2) = 8e-40 Identities = 12/15 (80%), Positives = 14/15 (93%) Frame = -1 Query: 51 AGRTDKGVTALQQVC 7 AGRTDKGV+AL Q+C Sbjct: 152 AGRTDKGVSALNQIC 166 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,391,615 Number of Sequences: 27288 Number of extensions: 244485 Number of successful extensions: 641 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 621 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 640 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 859346304 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18649446|gb|BM498265.1|BM498265 952028H03.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (609 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g26460.1 unknown protein / 192 1e-49 >At2g26460.1 unknown protein / Length = 585 Score = 192 bits (488), Expect = 1e-49 Identities = 96/147 (65%), Positives = 112/147 (75%), Gaps = 1/147 (0%) Frame = +3 Query: 171 MSSKKNYYXXXXXXXXXXXXXXPETPRYRDRAKERREDQNPDYEPTELGSFHAVAPPGN- 347 M K+++ + P+YRDRAKERRE+QNPDY+P+EL SFHAVAPPG Sbjct: 1 MKPSKSHHKEKTARRREEKLEESDNPKYRDRAKERRENQNPDYDPSELSSFHAVAPPGAV 60 Query: 348 DLRLADAHKISIEKSKYLGGDLEHTHLVKGLDYALLHKVRSEIEKKPEAEDGKDTKSRAA 527 D+R ADA KISIE SKYLGGD+EHTHLVKGLDYALL+KVRSEI KKP+ EDG K+ A Sbjct: 61 DIRAADALKISIENSKYLGGDVEHTHLVKGLDYALLNKVRSEIVKKPDGEDGDGGKTSAP 120 Query: 528 KEDQAVSFRTATAKSVYQWIIKPQSII 608 KEDQ V+FRT AKSVYQWI+KPQ+II Sbjct: 121 KEDQRVTFRTIAAKSVYQWIVKPQTII 147 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,207,306 Number of Sequences: 27288 Number of extensions: 236457 Number of successful extensions: 1877 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 1183 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1757 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 966764592 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18649284|gb|BM498103.1|BM498103 952022F10.x2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (568 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g47610.1 putative protein / activating signal cointegrator... 239 9e-64 >At3g47610.1 putative protein / activating signal cointegrator 1 - Homo sapiens, EMBL:AF168418; supported by full-length cDNA: Ceres:13265. Length = 408 Score = 239 bits (609), Expect = 9e-64 Identities = 117/181 (64%), Positives = 145/181 (79%) Frame = -2 Query: 567 KKGAKVISLAEAAKGSIVFKQGKPCSCQARQHDLVSSCLSCGKIVCEQEGEGPCSFCGAL 388 KKG KVISLAEAAKGSIVF+QGKPC+CQAR+H LVS+CLSCGKIVCEQEGEGPCSFCGAL Sbjct: 136 KKGGKVISLAEAAKGSIVFQQGKPCACQARRHHLVSNCLSCGKIVCEQEGEGPCSFCGAL 195 Query: 387 VLKEGSTYAGLSDVGLPLSEAETAAEAYAKRLVDYDRNAAARTKVYDDQSDYYEMEGNSW 208 VLKEGSTYAGL P+S+A+ AAEAYAKRLV+YDRN+AART V DDQSDYYE E ++W Sbjct: 196 VLKEGSTYAGLEVGYTPVSDADVAAEAYAKRLVEYDRNSAARTTVIDDQSDYYESESSTW 255 Query: 207 LSSKERSVLXXXXXXXXXXXXXXXXKVIVTFDLVGRKVILNKDGSTEVETEHPIMRRPKR 28 LS++E+ ++ KV++TFDL+GRKV+LN+D +E+E+ + I+ P+ Sbjct: 256 LSAEEKELVRKKREEIEEAERVKKSKVVMTFDLIGRKVLLNEDDISELESGNRILGPPET 315 Query: 27 R 25 + Sbjct: 316 K 316 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,664,277 Number of Sequences: 27288 Number of extensions: 226911 Number of successful extensions: 750 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 681 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 750 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 841443256 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18450668|gb|BM428946.1|BM428946 952026B02.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (573 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g35630.1 similar to ch-TOG protein from Homo sapiens / ; ... 291 2e-79 >At2g35630.1 similar to ch-TOG protein from Homo sapiens / ; supported by cDNA: gi_14317952_gb_AF367246.1_AF367246 Length = 1611 Score = 291 bits (745), Expect = 2e-79 Identities = 143/176 (81%), Positives = 158/176 (89%) Frame = -3 Query: 571 FVVLINLLRPLDPSRWPSPTPMESLAVKNQKFSDLVVKCLIKLPKVLQSXIYEVDLDRIL 392 FVVLI+LLRPLDPSRWPSP E AV+NQKFSDLVVKCLIKL K+LQS IYEVDLDR+L Sbjct: 1241 FVVLISLLRPLDPSRWPSPATAEVYAVRNQKFSDLVVKCLIKLTKLLQSTIYEVDLDRLL 1300 Query: 391 QSIHIYLQELGMEEIRRRAGADDKPLRMVKTVLHELVKLRGTAIKGHLSMVPIDAEPQPI 212 QSIH+YLQ+LGMEEIRRRAGADDKPLRMVKTVLHELVKLRG AIKGHLS+VPID PQPI Sbjct: 1301 QSIHVYLQDLGMEEIRRRAGADDKPLRMVKTVLHELVKLRGAAIKGHLSLVPIDMRPQPI 1360 Query: 211 ILAYIDLNLQTLAAARMLMPSGPMGQTHWGDAASNNPNPSTHSADAQLKQELAAVF 44 ILAYIDLNL+TLAAARML +GP+GQTHW D+ +NNP+P +SAD QLKQEL A+F Sbjct: 1361 ILAYIDLNLETLAAARMLTATGPVGQTHWTDSTANNPSPPANSADVQLKQELGAIF 1416 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,628,778 Number of Sequences: 27288 Number of extensions: 231665 Number of successful extensions: 628 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 606 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 627 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 859346304 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18385249|gb|BM418448.1|BM418448 952016F11.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (525 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g49550.1 Expressed protein / ; supported by full-length cD... 135 1e-32 >At5g49550.1 Expressed protein / ; supported by full-length cDNA: Ceres: 31276. Length = 127 Score = 135 bits (340), Expect = 1e-32 Identities = 64/106 (60%), Positives = 88/106 (82%) Frame = +2 Query: 92 DELSESLAELFTNVSLMVRGELQGTNNQLALLEKMNDRVSQEYSNYGDIAAGLRVFVEQL 271 D+L+ESL LFT+VS MV+ ELQGTNN L LLEKMN RV+ EY + GD+AAGLRVF EQ+ Sbjct: 6 DDLAESLQNLFTSVSSMVKSELQGTNNHLDLLEKMNLRVASEYDDMGDVAAGLRVFAEQM 65 Query: 272 NEKNRGFDDYVSQIDAIDQQVTEFEAVVSMLDKHVALLEKKVKSAY 409 K+ G D++V Q+DAI++QV+EFEAV+S+LD++V++LE K+++ Y Sbjct: 66 KSKSGGLDEFVGQMDAIEKQVSEFEAVISVLDRYVSVLESKIRAEY 111 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,983,996 Number of Sequences: 27288 Number of extensions: 186455 Number of successful extensions: 549 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 534 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 549 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 727283772 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18385146|gb|BM418345.1|BM418345 952014G06.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (286 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g58450.1 putative protein / predicted proteins from D.mela... 113 2e-30 >At5g58450.1 putative protein / predicted proteins from D.melanogaster, C.elegans and S.pombe Length = 1035 Score = 113 bits (283), Expect(2) = 2e-30 Identities = 59/77 (76%), Positives = 66/77 (85%) Frame = +2 Query: 56 PIWDAVDSRQYKPALKLCTALLAKHPTSPYALALKALILERMGKPDEALSGPLNAKELLY 235 PIWDA+DSRQ+K ALKL T+LLAK+P SPYALALKALI ERMGK DEALS L+AKELLY Sbjct: 81 PIWDAIDSRQFKNALKLVTSLLAKYPKSPYALALKALIHERMGKTDEALSVCLDAKELLY 140 Query: 236 SDNIFHFDDLTLSTLQI 286 D++ DDLTLSTLQI Sbjct: 141 KDDLALMDDLTLSTLQI 157 Score = 34.3 bits (77), Expect(2) = 2e-30 Identities = 15/16 (93%), Positives = 16/16 (99%) Frame = +1 Query: 4 MASKFGLAGGIPERRV 51 M+SKFGLAGGIPERRV Sbjct: 64 MSSKFGLAGGIPERRV 79 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,610,928 Number of Sequences: 27288 Number of extensions: 90684 Number of successful extensions: 252 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 242 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 252 length of database: 11,516,596 effective HSP length: 70 effective length of database: 9,606,436 effective search space used: 230554464 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18385136|gb|BM418335.1|BM418335 952014E12.x1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (615 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g31340.1 unknown protein / 125 2e-29 >At2g31340.1 unknown protein / Length = 258 Score = 125 bits (313), Expect = 2e-29 Identities = 60/123 (48%), Positives = 81/123 (65%) Frame = -2 Query: 614 SVVNPAMEKALKMLQEFASDVREGKVPKIRSSFGAPWRHPPRDDNPDLSYKWAKIQLMDF 435 +VVNPAM+KAL LQ +A+DVR+G +P + FGA WRHPP+ ++P + +WAKIQLMDF Sbjct: 62 AVVNPAMDKALLRLQRYATDVRKGSIPYEKLRFGASWRHPPQTEDPTQNSEWAKIQLMDF 121 Query: 434 IQSFVNTEFGVNYLADDSLEILDDPAAVAMMEVGLLYQQREPSFMRPITRGIQRCLARWL 255 +Q+ VNTEF VG+LY QR+PSF RPI++GI+RCL RW Sbjct: 122 VQALVNTEFA----------------------VGILYTQRDPSFFRPISQGIKRCLVRWN 159 Query: 254 AEQ 246 ++ Sbjct: 160 VDE 162 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,089,529 Number of Sequences: 27288 Number of extensions: 298782 Number of successful extensions: 864 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 843 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 863 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 972741724 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18384602|gb|BM417801.1|BM417801 952005G08.x8 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (477 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g20990.1 MOLYBDOPTERIN BIOSYNTHESIS CNX1 PROTEIN / ; supp... 163 4e-41 >At5g20990.1 MOLYBDOPTERIN BIOSYNTHESIS CNX1 PROTEIN / ; supported by cDNA: gi_1263313_gb_L47323.1_ATHCNX1R Length = 670 Score = 163 bits (413), Expect = 4e-41 Identities = 87/145 (60%), Positives = 101/145 (69%) Frame = +1 Query: 43 PHAVPLQGALGLVLAEDVCAPDPLPPFRASIKDGYAVVASDGPGEYPVVTESRAGDDALX 222 P V L ALG VLAED+ APDPLPP+ AS+KDGYAVVASDGPGEYPV+TESRAG+D L Sbjct: 35 PVIVSLYEALGKVLAEDIRAPDPLPPYPASVKDGYAVVASDGPGEYPVITESRAGNDGLG 94 Query: 223 XXXXXXXXXXXXXXXXXXDGADAVVQVEDTEQLPGGADGSKRVRILARASQRQDIRSVGC 402 DGADAVVQVEDT+ + + SKRV+IL + + DIR VGC Sbjct: 95 VTVTPGTVAYVTTGGPIPDGADAVVQVEDTKVIGDVSTESKRVKILIQTKKGTDIRRVGC 154 Query: 403 DIEKDSIVLKSGEHIGPAEIGLLAT 477 DIEKD+ VL +GE IG +EIGLLAT Sbjct: 155 DIEKDATVLTTGERIGASEIGLLAT 179 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.313 0.137 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,887,877 Number of Sequences: 27288 Number of extensions: 168755 Number of successful extensions: 692 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 564 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 643 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 605236996 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18384108|gb|BM417308.1|BM417308 952008F09.x4 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (573 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g54855.1 Expressed protein / ; supported by full-length cD... 201 2e-52 >At5g54855.1 Expressed protein / ; supported by full-length cDNA: Ceres: 9159. Length = 146 Score = 201 bits (511), Expect = 2e-52 Identities = 91/121 (75%), Positives = 103/121 (84%) Frame = -1 Query: 528 AWTGEIRGRVVCDVCGDAAIGPEXHALEGAEVAVLCITKSGEVINYQAFTNSKGVYTVAE 349 AWTGEIRGRVVCDVC D++IGPE H LEGAEVAVLCITKSGEV+NYQAFTNSKGVYTVAE Sbjct: 26 AWTGEIRGRVVCDVCADSSIGPEDHVLEGAEVAVLCITKSGEVVNYQAFTNSKGVYTVAE 85 Query: 348 TMPESDRWDSCLARPMSSFHLHCTRRGDAHSGVKFTYNKSSGNPHTVKPFLYKPATVPLY 169 TMPES+RWD+CLARP+SSFH C R ++G+KFTYN+ SG H VKPF+Y+P P Y Sbjct: 86 TMPESERWDACLARPISSFHTPCNRLHQTNTGIKFTYNRPSGFFHAVKPFVYRPQYAPSY 145 Query: 168 C 166 C Sbjct: 146 C 146 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,576,761 Number of Sequences: 27288 Number of extensions: 249834 Number of successful extensions: 655 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 639 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 655 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 859346304 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18383990|gb|BM417190.1|BM417190 952007B07.x2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (457 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g51270.1 putative protein / several hypothetical proteins ... 189 6e-49 >At3g51270.1 putative protein / several hypothetical proteins of different species Length = 472 Score = 189 bits (480), Expect = 6e-49 Identities = 102/143 (71%), Positives = 110/143 (76%), Gaps = 11/143 (7%) Frame = +2 Query: 62 MKLDVNALRYLAKDDFRVLTAVEMGMRNHEIVPAELVDRIARLKHGGTYXXXXXXXXXXX 241 MKLDVN LRYL+KDDFRVLTAVEMGMRNHEIVP+ELV+RIA LKHGGTY Sbjct: 1 MKLDVNVLRYLSKDDFRVLTAVEMGMRNHEIVPSELVERIACLKHGGTYKVLKNLLKIGS 60 Query: 242 X----HHDATKY-------DGYRLTYLGYDFLAIKTLXNRGVFASVGRQIGVGKESDIFE 388 + A + DG+RLTYLGYDFLAIKTL NRG+F VGRQIGVGKESDIFE Sbjct: 61 LVKLQNLIAIAFFVYICLDDGFRLTYLGYDFLAIKTLVNRGIFTGVGRQIGVGKESDIFE 120 Query: 389 VATEDGTVLAMKLHRLGRTSFRA 457 VA EDGT+LAMKLHRLGRTSFRA Sbjct: 121 VAQEDGTILAMKLHRLGRTSFRA 143 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,297,427 Number of Sequences: 27288 Number of extensions: 173523 Number of successful extensions: 470 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 461 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 470 length of database: 11,516,596 effective HSP length: 90 effective length of database: 9,060,676 effective search space used: 552701236 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18383981|gb|BM417181.1|BM417181 952007A05.x2 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (343 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g21140.1 hypothetical protein / predicted by genscan+ 152 2e-38 >At5g21140.1 hypothetical protein / predicted by genscan+ Length = 287 Score = 152 bits (385), Expect = 2e-38 Identities = 67/113 (59%), Positives = 93/113 (82%) Frame = +1 Query: 4 RHHTLLQALLHRGPLSERDFHAVFAGVSGKNPATHHQLFNDTLLKINNDLAYLQFELRAC 183 +HHTL+QAL+ RGPL E++FH++F V+G+NP T ++F+ LL+IN +L+Y+ FEL+AC Sbjct: 7 KHHTLIQALISRGPLKEKEFHSIFTAVTGRNPVTVKKIFDKYLLEINKELSYVHFELKAC 66 Query: 184 INQYDGMVYYGVVNNIADEESKLGTKYSVPQIAFYKGLLEAIVQEAGNDGSIT 342 +QYDG V YGVVNN+AD++SKLGTKYSVPQIAF+KG++EAI Q+ G I+ Sbjct: 67 RDQYDGQVCYGVVNNVADDQSKLGTKYSVPQIAFFKGIIEAIAQDEAAQGCIS 119 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,824,132 Number of Sequences: 27288 Number of extensions: 128756 Number of successful extensions: 367 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 358 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 367 length of database: 11,516,596 effective HSP length: 89 effective length of database: 9,087,964 effective search space used: 218111136 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18383975|gb|BM417175.1|BM417175 952005H09.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (537 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g63660.1 GMP synthase / similar to GMP synthase GB:6323873... 277 3e-75 >At1g63660.1 GMP synthase / similar to GMP synthase GB:6323873 [Saccharomyces cerevisiae] Length = 534 Score = 277 bits (708), Expect = 3e-75 Identities = 134/161 (83%), Positives = 148/161 (91%) Frame = +3 Query: 54 GGLPKDMKLKLIEPLKLLFKDEVRKLGGILNVPDSFLKRHPFPGPGLAVRVLGDVTQGNA 233 GGLPKDMKLKLIEPLKLLFKDEVR+LG ILNVP FLKRHPFPGPGLAVRVLGDVTQGNA Sbjct: 370 GGLPKDMKLKLIEPLKLLFKDEVRELGRILNVPVGFLKRHPFPGPGLAVRVLGDVTQGNA 429 Query: 234 LEVLRQVDEIFVQSIKDAGLYDKIWQAFAVFLPVQTVGVQGDQRTHSNAVVLRAITSEDG 413 LEVLRQVDEIF+QSI+DAGLYD IWQAFAVFLPV++VGVQGD+RTHS+ V LRA+TS+DG Sbjct: 430 LEVLRQVDEIFIQSIRDAGLYDSIWQAFAVFLPVRSVGVQGDKRTHSHVVALRAVTSQDG 489 Query: 414 MTADWYYFEHAFLVDVVNKICNNVRGINRVCQDITSKPPAT 536 MTADW+ FEH FL DV KICN+V+G+NRV DITSKPP+T Sbjct: 490 MTADWFNFEHKFLDDVSRKICNSVQGVNRVVLDITSKPPST 530 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,996,873 Number of Sequences: 27288 Number of extensions: 294306 Number of successful extensions: 706 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 686 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 706 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 763199020 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18383971|gb|BM417171.1|BM417171 952005H01.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (355 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g17050.1 hypothetical protein / 84 1e-17 >At4g17050.1 hypothetical protein / Length = 298 Score = 84.3 bits (207), Expect = 1e-17 Identities = 37/39 (94%), Positives = 38/39 (96%) Frame = +3 Query: 3 PVQAGDTIWMAPFVPQWYAALGKTRTRYLLYKDVNRNPL 119 PVQAGD IWMAPFVPQWYAALGKTR+RYLLYKDVNRNPL Sbjct: 260 PVQAGDVIWMAPFVPQWYAALGKTRSRYLLYKDVNRNPL 298 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,040,948 Number of Sequences: 27288 Number of extensions: 159110 Number of successful extensions: 369 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 369 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 369 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 215491488 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18383923|gb|BM417123.1|BM417123 952005A06.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (360 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g50410.1 unknown protein / 102 4e-23 >At5g50410.1 unknown protein / Length = 259 Score = 102 bits (253), Expect = 4e-23 Identities = 53/135 (39%), Positives = 79/135 (58%), Gaps = 16/135 (11%) Frame = +1 Query: 4 PPHPWEISARAWLESFPDGRPPTEP-EVDAYIDVHRPELPS----LPRSQLHQRLLALR- 165 P WE ARAW+ +FPD + EV+ +I + LP+ +PRS++ RLL+++ Sbjct: 34 PSQEWETMARAWVSAFPDAKAVVSAGEVETWIGTNLDSLPADLRHMPRSEIVDRLLSIQH 93 Query: 166 ----------GDQVLDADQSAFPYRFQRTDLWKPVYQWLESLELDSLVATQQISDWLTSN 315 +Q ++ADQ P RFQRTD W PVY WLESL+ LV ++ IS+WL +N Sbjct: 94 YMRPTPSSDQNEQNVEADQQ-HPARFQRTDQWLPVYSWLESLDNGELVKSKDISEWLDAN 152 Query: 316 PKIMDRMVEKHSKYH 360 P++ + +HS+YH Sbjct: 153 PEVKQELSSRHSRYH 167 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,787,762 Number of Sequences: 27288 Number of extensions: 118671 Number of successful extensions: 383 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 359 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 379 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 214181664 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18383893|gb|BM417093.1|BM417093 952003E04.y1 952 - BMS tissue from Walbot Lab (reduced rRNA) Zea mays cDNA, mRNA sequence (625 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g03380.1 DegP protease / contains similarity to degP GI:26... 289 7e-79 >At3g03380.1 DegP protease / contains similarity to degP GI:2623992 from [Bradyrhizobium japonicum] Length = 627 Score = 289 bits (740), Expect = 7e-79 Identities = 147/201 (73%), Positives = 162/201 (80%) Frame = -3 Query: 623 DFGFFRYDPGAIKFLKYDEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDREAPY 444 DFGFF YDP A++FL Y EIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDR+AP+ Sbjct: 109 DFGFFCYDPSAVQFLTYQEIPLAPEAASVGLEIRVVGNDSGEKVSILAGTLARLDRDAPH 168 Query: 443 YKKDGYNDFNTFYMQAAXXXXXXXXXSPVVDCQGRAVALNXXXXXXXXXAFFLPLERVVR 264 YKKDGYNDFNTFYMQAA SPV+D QGRAVALN AFFLPL+RVVR Sbjct: 169 YKKDGYNDFNTFYMQAASGTKGGSSGSPVIDWQGRAVALNAGSKSSSASAFFLPLQRVVR 228 Query: 263 ALDLIRDSWDAFGTKTESVYIPRGTLQATFQHKGFEETRRLGLRNETEQMVRVVSPEGET 84 AL ++ S D+ K ++V+IPRGTLQ TF HKGF+E RRLGLR+ETEQ+VR SP GET Sbjct: 229 ALSFLQKSIDSRTDKPKAVHIPRGTLQMTFLHKGFDEIRRLGLRSETEQVVRHASPTGET 288 Query: 83 GMLVVDSVVPKGPAHKHLEPG 21 GMLVVDSVVP GPA KHLEPG Sbjct: 289 GMLVVDSVVPSGPADKHLEPG 309 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,738,252 Number of Sequences: 27288 Number of extensions: 297571 Number of successful extensions: 824 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 802 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 821 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 999514432 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18181500|gb|BM382710.1|BM382710 MEST553-F08.univ ISUM6 Zea mays cDNA clone MEST553-F08 3', mRNA sequence (491 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g16070.1 expressed protein / ;supported by full-length cDN... 61 3e-10 >At2g16070.1 expressed protein / ;supported by full-length cDNA: Ceres:95679. Length = 307 Score = 61.2 bits (147), Expect = 3e-10 Identities = 25/43 (58%), Positives = 34/43 (78%) Frame = -3 Query: 474 RRAPLRCPPGKAMVVENGRARCVVKERVEVPFDAVLAPPSSRY 346 +R P +CPPGK +V+E+G ARC+VKERVE+PFD+V+A Y Sbjct: 262 KRTPNQCPPGKVLVIEDGEARCLVKERVEIPFDSVVAKRDVTY 304 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,772,723 Number of Sequences: 27288 Number of extensions: 230245 Number of successful extensions: 827 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 766 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 820 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 639433100 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18181281|gb|BM382491.1|BM382491 MEST550-D12.univ ISUM6 Zea mays cDNA clone MEST550-D12 3', mRNA sequence (640 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g45760.1 G-protein beta family / ;supported by full-lengt... 172 1e-43 >At5g45760.1 G-protein beta family / ;supported by full-length cDNA: Ceres:104220. Length = 360 Score = 172 bits (437), Expect = 1e-43 Identities = 95/186 (51%), Positives = 118/186 (63%) Frame = -3 Query: 635 LREAHTSICSSVQFIPWRPWTAITGGLDSKLAAWDFSKGRTLFSIDYGSPELQNGSSSGS 456 LR HTSICSSVQFIPWRPW ITGGLDSKL WDFSKGR+ ID+GS ++ + Sbjct: 143 LRAGHTSICSSVQFIPWRPWEVITGGLDSKLVLWDFSKGRSQKIIDFGS------DTNNN 196 Query: 455 AGQCFNPAFVHSVAVSEEGILGGLYKXXXXXXXXXXXXXXDLEYELAPAKSKGPPRAAIL 276 +GQC NPAFVHS+A+ E ++ L K ++E EL+ KG Sbjct: 197 SGQCLNPAFVHSIAIPEMDMVDKLDKICAVARGDGIVNLINIESELS---RKG------- 246 Query: 275 TTRSKGAELGDGSCNQSRAKRIHLDYTMGGHTAAVSCVAFSAFGEKGKFLISGGNDASVK 96 T SKG+ S + + KR+ LDY++GGH +AVSCVAFS F EKG+FLISGGND +VK Sbjct: 247 -TSSKGS---SSSSSNNVVKRVCLDYSVGGHNSAVSCVAFSLFQEKGRFLISGGNDKTVK 302 Query: 95 LWDWSK 78 +WD K Sbjct: 303 IWDCFK 308 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,071,064 Number of Sequences: 27288 Number of extensions: 267727 Number of successful extensions: 1031 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 855 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1025 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 1044135612 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18180917|gb|BM382127.1|BM382127 MEST545-A07.univ ISUM6 Zea mays cDNA clone MEST545-A07 3', mRNA sequence (423 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g01300.1 putative protein / several hypothetical proteins ... 106 5e-24 >At5g01300.1 putative protein / several hypothetical proteins - different bacteria; supported by cDNA: gi_20465384 Length = 162 Score = 106 bits (264), Expect = 5e-24 Identities = 51/88 (57%), Positives = 65/88 (72%) Frame = -2 Query: 422 IPPATRGLPEGFSGAAGAGPGRDQLGGLQEGVNDWKQPGWRGPVPPSHGHRIQFKLYALD 243 IPP +GLPEG+SG DQ G++EG ND K PGWRGP+ PSHGHR QFKL+ALD Sbjct: 77 IPPEMKGLPEGYSG------NEDQTTGIREGNNDHKIPGWRGPLLPSHGHRFQFKLFALD 130 Query: 242 DEVHLGNKVTRDKLMEAIDGHVLEEAEL 159 D+ +G+ VT+++L+ AI+GHVL EA L Sbjct: 131 DKPKIGHTVTKERLLIAIEGHVLGEAIL 158 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,836,983 Number of Sequences: 27288 Number of extensions: 135920 Number of successful extensions: 831 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 631 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 809 length of database: 11,516,596 effective HSP length: 89 effective length of database: 9,087,964 effective search space used: 463486164 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18180736|gb|BM381946.1|BM381946 MEST542-D01.univ ISUM6 Zea mays cDNA clone MEST542-D01 3', mRNA sequence (493 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g13070.1 putative protein / CGI-107 protein - Homo sapiens... 105 1e-23 >At5g13070.1 putative protein / CGI-107 protein - Homo sapiens, EMBL:AF151865 Length = 183 Score = 105 bits (262), Expect = 1e-23 Identities = 49/89 (55%), Positives = 62/89 (69%) Frame = -3 Query: 488 RAMDVVVRNVSLRGLIEVEERASYRPHPDRPDDWTQFRQETTIRCRPXXXXXXXXXXXET 309 R+M + +N+SL+ IEVEER Y PHPD P WT QET+IR +P E Sbjct: 93 RSMQLTTKNISLKKFIEVEERIRYDPHPDNPSAWTVCSQETSIRIKPLSALASMAEKVEQ 152 Query: 308 RCAERFLQNSAKGREVVERICRYLEAESA 222 +CAE+F+QNSAKGREV+ERIC+Y+EAESA Sbjct: 153 KCAEKFMQNSAKGREVMERICKYMEAESA 181 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,464,739 Number of Sequences: 27288 Number of extensions: 124696 Number of successful extensions: 330 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 328 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 330 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 639433100 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18180489|gb|BM381699.1|BM381699 MEST538-G12.univ ISUM6 Zea mays cDNA clone MEST538-G12 3', mRNA sequence (566 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g52780.1 putative protein / similar to unknown protein (pi... 107 4e-24 >At5g52780.1 putative protein / similar to unknown protein (pir||T04431);supported by full-length cDNA: Ceres:26264. Length = 168 Score = 107 bits (267), Expect = 4e-24 Identities = 53/114 (46%), Positives = 73/114 (63%) Frame = -2 Query: 523 EASLAKQDGGGSNGEVDDDDELPQPVFDRILRRIAFTVGLPMASGVALLNVYDALKRGQG 344 ++S + G + + +DD +PQ VF+R++ RI +VG P+ GVA+L V + LK Sbjct: 51 QSSRTSEKPGRPDPDPEDDPPIPQEVFERMMGRIVVSVGTPLGLGVAILKVLEVLKDRNV 110 Query: 343 VVVPSWVPLLTILVAFGTSALGIAYGTLSASWDPDKEGSLLGIDEARANWPVLW 182 VP WVP LT LV FG+SALGIAYG+LS + DP K SL G+ EA+ NW +W Sbjct: 111 WDVPLWVPYLTTLVTFGSSALGIAYGSLSTNLDPAKTNSLFGLKEAKENWVEMW 164 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,204,677 Number of Sequences: 27288 Number of extensions: 157444 Number of successful extensions: 749 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 720 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 748 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 841443256 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18180308|gb|BM381518.1|BM381518 MEST535-G11.univ ISUM6 Zea mays cDNA clone MEST535-G11 3', mRNA sequence (591 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g66090.1 expressed protein / ;supported by full-length cD... 215 1e-56 >At5g66090.1 expressed protein / ;supported by full-length cDNA: Ceres:124621. Length = 210 Score = 215 bits (548), Expect = 1e-56 Identities = 104/130 (80%), Positives = 120/130 (92%) Frame = -2 Query: 587 VKHVLLPVLDRNPYLSEGTRQAAATTASLAKKYGADITVVVIDDKPKESVPEHDTQMSSI 408 VKH+LLPV+DRNPYLSEGTRQAAATT SLAKKYGADITVVVID++ +ES EH+TQ+S+I Sbjct: 81 VKHLLLPVIDRNPYLSEGTRQAAATTTSLAKKYGADITVVVIDEEKRESSSEHETQVSNI 140 Query: 407 RWHLSEGGFSEFRLMERLGEGQKPTVIIGEVADEWDLDLVVLSMEAIHSKHVDGNLLAEF 228 RWHLSEGGF EF+L+ERLGEG+K T IIGEVADE ++LVV+SMEAIHSK++D NLLAEF Sbjct: 141 RWHLSEGGFEEFKLLERLGEGKKATAIIGEVADELKMELVVMSMEAIHSKYIDANLLAEF 200 Query: 227 IPCPVLLLPL 198 IPCPVLLLPL Sbjct: 201 IPCPVLLLPL 210 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,471,454 Number of Sequences: 27288 Number of extensions: 270295 Number of successful extensions: 785 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 767 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 784 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 913055448 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179945|gb|BM381155.1|BM381155 MEST530-F06.univ ISUM6 Zea mays cDNA clone MEST530-F06 3', mRNA sequence (533 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g14615.1 Expressed protein / ; supported by full-length cD... 54 5e-08 >At4g14615.1 Expressed protein / ; supported by full-length cDNA: Ceres: 42223. Length = 76 Score = 53.9 bits (128), Expect = 5e-08 Identities = 24/38 (63%), Positives = 29/38 (76%) Frame = -2 Query: 532 GTSKGILEIAKFGVYVSVPVALTYLVATDSKTLKKLMG 419 GTSKG+LEI KFGVYV+VP+ L Y A +S +KK MG Sbjct: 5 GTSKGVLEIVKFGVYVAVPIVLMYTFANNSTNIKKFMG 42 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,516,584 Number of Sequences: 27288 Number of extensions: 147058 Number of successful extensions: 345 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 340 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 345 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 754220208 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179906|gb|BM381116.1|BM381116 MEST530-B06.univ ISUM6 Zea mays cDNA clone MEST530-B06 3', mRNA sequence (461 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g57540.1 hypothetical protein / contains similarity to T-b... 148 1e-36 >At1g57540.1 hypothetical protein / contains similarity to T-box domain GI:5931592 from (Cynops pyrrhogaster) Length = 103 Score = 148 bits (374), Expect = 1e-36 Identities = 66/84 (78%), Positives = 78/84 (92%) Frame = -2 Query: 460 SEGEVMRPESKPCSRLMRQTAGIFSVGGGLAFWVLCRLHYGPRITVPRSLRWASCGAVSM 281 SEG++MR ++KPCSRLM+QTA IF+VGG L FWVLCRLHYGPRITVPRSLRWA CGA+S+ Sbjct: 10 SEGDLMRSDAKPCSRLMKQTAAIFTVGGALGFWVLCRLHYGPRITVPRSLRWAGCGALSV 69 Query: 280 SATSALLVRLFSPECEPQNIAAFD 209 S ++A+LVRLFSPECEPQNIAA+D Sbjct: 70 STSTAMLVRLFSPECEPQNIAAYD 93 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,818,844 Number of Sequences: 27288 Number of extensions: 228254 Number of successful extensions: 615 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 610 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 615 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 560070056 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179790|gb|BM381000.1|BM381000 MEST528-D06.univ ISUM6 Zea mays cDNA clone MEST528-D06 3', mRNA sequence (481 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g08170.1 putative protein / hypothetical bacterial proteins 80 5e-16 >At5g08170.1 putative protein / hypothetical bacterial proteins Length = 383 Score = 80.1 bits (196), Expect = 5e-16 Identities = 40/61 (65%), Positives = 43/61 (69%), Gaps = 1/61 (1%) Frame = -1 Query: 481 IANGGIIAPSFGDRW-DEEAYAVLQKAFPDHEVVMVEGGREIALGGGNIHCITQQQPVRP 305 IANGGIIAP FGD D+EA VL FP H VV +E REI L GGNIHCITQQQP P Sbjct: 316 IANGGIIAPQFGDPIRDKEAIRVLSDTFPHHSVVGIENAREIVLAGGNIHCITQQQPAEP 375 Query: 304 S 302 + Sbjct: 376 T 376 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,167,580 Number of Sequences: 27288 Number of extensions: 166917 Number of successful extensions: 476 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 472 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 475 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 614270384 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179784|gb|BM380994.1|BM380994 MEST528-C08.univ ISUM6 Zea mays cDNA clone MEST528-C08 3', mRNA sequence (579 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g40500.1 hypothetical protein / ; supported by cDNA: gi_1... 133 7e-32 >At5g40500.1 hypothetical protein / ; supported by cDNA: gi_17644174 Length = 123 Score = 133 bits (334), Expect = 7e-32 Identities = 58/81 (71%), Positives = 69/81 (84%) Frame = -2 Query: 473 HPFKVVEITPPPRCLGVRCFPTNIHCGESVTIEGQAYTVSAVTHRYQLRKGRYEPSEKRL 294 +P+KV+EITPPP+ LG+RC P N+ CGE+V IEGQ YT+SAVTHRYQLRKG+YEPSE+RL Sbjct: 43 YPYKVIEITPPPKSLGIRCLPHNLQCGENVMIEGQTYTISAVTHRYQLRKGKYEPSERRL 102 Query: 293 DVLSTGRYILNLYLQNLLDQS 231 DVLS RY+LNLY NLL S Sbjct: 103 DVLSAARYVLNLYFDNLLQNS 123 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,726,734 Number of Sequences: 27288 Number of extensions: 184631 Number of successful extensions: 557 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 529 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 554 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 877249352 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179731|gb|BM380941.1|BM380941 MEST527-E07.univ ISUM6 Zea mays cDNA clone MEST527-E07 3', mRNA sequence (568 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g20180.1 ribosomal protein L36-like / ribosomal protein L3... 56 1e-08 >At5g20180.1 ribosomal protein L36-like / ribosomal protein L36 - Synechocystis sp., PIR:S77481;supported by full-length cDNA: Ceres:28109. Length = 103 Score = 55.8 bits (133), Expect = 1e-08 Identities = 35/93 (37%), Positives = 45/93 (47%), Gaps = 6/93 (6%) Frame = -2 Query: 498 LCRFCKVVKRRGIVFVQCTANAKHKQRQGFSTIAEAAAVSCVHLXXXXXXXXXXXXXAFA 319 +C FCK VKRRG V+V C++N KHKQRQGFS+ A Sbjct: 10 MCEFCKTVKRRGRVYVICSSNPKHKQRQGFSSFAYEGITPSPLFAEPIASQELVRLPGQG 69 Query: 318 ETSKVA-----RQEMSMKFNWRLGLAA-LFKNG 238 ++ +A R + F WR GLA+ LFK G Sbjct: 70 VSAGLASLLHKRPMPTAYFGWRSGLASILFKQG 102 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,486,528 Number of Sequences: 27288 Number of extensions: 165478 Number of successful extensions: 450 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 435 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 450 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 841443256 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179710|gb|BM380920.1|BM380920 MEST527-B09.univ ISUM6 Zea mays cDNA clone MEST527-B09 3', mRNA sequence (655 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g09620.1 putative protein / hypothetical protein F6E13.15 ... 195 2e-50 >At4g09620.1 putative protein / hypothetical protein F6E13.15 - Arabidopsis thaliana,PIR2:T00682 Length = 210 Score = 195 bits (496), Expect = 2e-50 Identities = 87/133 (65%), Positives = 109/133 (81%) Frame = -1 Query: 625 EECKRVLTPLGFSSEDAEKMLKKAFGWTHSPYWSEEREKEVPTAEAVAGVLDYIRSLGLS 446 EECK L+ FS E+ +K+L KAFG HSPYW+EER KE P E + +L+++RSLGLS Sbjct: 78 EECKEALSCFDFSVEEKDKILGKAFGHIHSPYWTEERVKENPKVETLNQILEFLRSLGLS 137 Query: 445 DEDLGRLLKKFPEVLGCDLDGEVRPNVGKLDSDWGINGKTLRSLLLRNPKVLGYNVDCRG 266 DEDL +++KKFPEVLGC L+ E++PN+G L++ WGI GK LR+LLLRNPKVLGYNVDC+G Sbjct: 138 DEDLHKVMKKFPEVLGCSLEEEMKPNIGILENQWGITGKQLRNLLLRNPKVLGYNVDCKG 197 Query: 265 DCMAQCTRCWVRF 227 DC+AQCTRCWVRF Sbjct: 198 DCVAQCTRCWVRF 210 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,735,603 Number of Sequences: 27288 Number of extensions: 297463 Number of successful extensions: 1138 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 1069 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1132 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 1088756792 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179688|gb|BM380898.1|BM380898 MEST526-G09.univ ISUM6 Zea mays cDNA clone MEST526-G09 3', mRNA sequence (580 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g02530.1 chloroplast thylakoid lumen protein / SP:022773 ;... 122 2e-28 >At4g02530.1 chloroplast thylakoid lumen protein / SP:022773 ;TL16_ARATH; supported by full-length cDNA: Ceres: 8114. Length = 216 Score = 122 bits (305), Expect = 2e-28 Identities = 63/98 (64%), Positives = 76/98 (77%) Frame = -1 Query: 580 RLSKVGQAIDNDDLPAASSVLGPSSDAQWVQNINAAFSKLSSSPEQRNVVDSFNSSLATL 401 +LSKVGQAI+N+DLPAA VLG D +WV+ +N AF+KLS+SPE+ V++FNSSLA+L Sbjct: 118 KLSKVGQAIENNDLPAAGLVLGKGIDTEWVKTVNLAFTKLSTSPEENTEVEAFNSSLASL 177 Query: 400 FTSVSKLDPESSKSAFVSSATALEKWIALAGLGGQLKG 287 TSV+K D ESSK AFVSSA A EKW L GL QLKG Sbjct: 178 ITSVNKNDIESSKLAFVSSAGAFEKWTTLTGLLEQLKG 215 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,032,562 Number of Sequences: 27288 Number of extensions: 207754 Number of successful extensions: 661 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 636 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 661 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 877249352 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179661|gb|BM380871.1|BM380871 MEST526-C12.univ ISUM6 Zea mays cDNA clone MEST526-C12 3', mRNA sequence (546 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g60190.1 putative protein / predicted proteins, C.elegans ... 93 1e-19 >At5g60190.1 putative protein / predicted proteins, C.elegans and D.melanogaster Length = 226 Score = 92.8 bits (229), Expect = 1e-19 Identities = 53/141 (37%), Positives = 73/141 (51%) Frame = +1 Query: 121 EERVLFHGDVVLIRSDLAILRGPCFINDRIIAFYFAHXXXXXXXXXXXXXXXXXXXXXXN 300 ++++L + DVVL RSDL IL GP F+NDR+I FY + N Sbjct: 7 DDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSIAFWISN 66 Query: 301 LPDSAAVAAVAEPLRLASRRLGLLPVNDNADASIAEGGSHWTLLILDNITSPSVPRFVHH 480 PD+ + +PL L + L +LPVNDN++ +AEGG HW+LL+ FVHH Sbjct: 67 CPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVY----YKEANTFVHH 122 Query: 481 DSIAGAPNLPIAARLADALRP 543 DS G N A +L A+ P Sbjct: 123 DSYMGV-NRWSAKQLFKAVSP 142 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,048,078 Number of Sequences: 27288 Number of extensions: 194680 Number of successful extensions: 1237 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 1037 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1212 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 790135456 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179571|gb|BM380781.1|BM380781 MEST525-A09.univ ISUM6 Zea mays cDNA clone MEST525-A09 3', mRNA sequence (606 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g61500.1 autophagocytosis protein - like / autophagocytosi... 209 1e-54 >At5g61500.1 autophagocytosis protein - like / autophagocytosis protein AUT1, Saccharomyces cerevisiae, PIR:S45130;supported by full-length cDNA: Ceres:8184. Length = 313 Score = 209 bits (531), Expect = 1e-54 Identities = 101/116 (87%), Positives = 107/116 (92%) Frame = -1 Query: 606 SITYDKYYQTPRVWLTGYDESRMPLMPELVFEDISQDHARKTVTIEDHPHLLAGKHASVH 427 SITYDKYYQTPRVWLTGYDESRM L PELV ED+SQDHARKTVTIEDHPHL GKHASVH Sbjct: 198 SITYDKYYQTPRVWLTGYDESRMLLQPELVMEDVSQDHARKTVTIEDHPHL-PGKHASVH 256 Query: 426 PCKHAAVMKKIIDVPMSGGVEPEVDKYLFIFLKFIATVIPTIEYDYTMDVDLGSTS 259 PC+H AVMKKIIDV MS GVEPEVDKYLF+FLKF+A+VIPTIEYDYTMD DLGS+S Sbjct: 257 PCRHGAVMKKIIDVLMSRGVEPEVDKYLFLFLKFMASVIPTIEYDYTMDFDLGSSS 312 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,497,708 Number of Sequences: 27288 Number of extensions: 307672 Number of successful extensions: 743 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 728 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 742 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 957813068 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179445|gb|BM380655.1|BM380655 MEST523-A11.univ ISUM6 Zea mays cDNA clone MEST523-A11 3', mRNA sequence (665 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g08580.1 hypothetical protein / predicted by genscan 65 4e-11 >At1g08580.1 hypothetical protein / predicted by genscan Length = 123 Score = 64.7 bits (156), Expect = 4e-11 Identities = 42/100 (42%), Positives = 56/100 (56%) Frame = -2 Query: 646 MAKYNVVQKNKRQCNQDRKRAAHGEPGTGKLKQRTAPVSMSGXXXXXXXXXXXXXXXEAA 467 MAKYN + K KR+ DRKRA HG+P T KLK RT S+SG + Sbjct: 1 MAKYNEIAKKKREAKADRKRAIHGDPLTNKLKTRTPVPSVSGKRQRKLLRKWRREQKD-- 58 Query: 466 MIKALENNMGDVDMVSVEESSEAAKGKSQVKFSVKKNSRI 347 M++ M DV+M S + +SE +K KS KFSVKK+ ++ Sbjct: 59 MVEKGLVTMEDVEMASAQAASEDSK-KSPRKFSVKKSLKL 97 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,772,933 Number of Sequences: 27288 Number of extensions: 185785 Number of successful extensions: 521 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 509 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 521 length of database: 11,516,596 effective HSP length: 96 effective length of database: 8,896,948 effective search space used: 1112118500 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179239|gb|BM380449.1|BM380449 MEST520-A09.univ ISUM6 Zea mays cDNA clone MEST520-A09 3', mRNA sequence (549 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g12650.1 expressed protein / ; supported by cDNA: gi_16323... 60 6e-10 >At3g12650.1 expressed protein / ; supported by cDNA: gi_16323108_gb_AY057658.1_ Length = 238 Score = 60.5 bits (145), Expect = 6e-10 Identities = 27/51 (52%), Positives = 40/51 (77%) Frame = -3 Query: 418 LVLRRAIKPIAFFQKMEANARLQVLTLCLQATKNVNLMLLRTRVMAISCAL 266 L++RR++KP+ FF+KME RLQ+LTL LQ KN+NL+ +R RV++I C + Sbjct: 175 LMVRRSLKPMYFFKKMEELGRLQILTLTLQVAKNLNLLFVRARVVSILCVV 225 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,244,749 Number of Sequences: 27288 Number of extensions: 164383 Number of successful extensions: 429 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 421 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 429 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 799114268 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179159|gb|BM380369.1|BM380369 MEST518-H09.univ ISUM6 Zea mays cDNA clone MEST518-H09 3', mRNA sequence (692 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g52580.1 unknown protein / 190 5e-49 >At5g52580.1 unknown protein / Length = 327 Score = 190 bits (483), Expect = 5e-49 Identities = 101/175 (57%), Positives = 128/175 (72%) Frame = -3 Query: 603 RNLYTIKALPLSDVRFIRKYTPTFGLDYVIIVLSSGLAFPPFYFYNGGIRELFATLKQHV 424 R+LYTI A+P ++VR IR++TP G YVI+VLSSGLAFPP YFYNGG+RE A +KQHV Sbjct: 69 RSLYTITAVPFTEVRSIRRHTPALGWQYVIVVLSSGLAFPPLYFYNGGVREFLAMVKQHV 128 Query: 423 FIIRSDDDPNVFLVNDFQDPLQKSLSSLELPGVASVANAMSRQNSLSFTGSIDESRHGDN 244 F+ RS +D NVF+VNDFQ PLQ++LSSLELP VA+ S + S ++ R + Sbjct: 129 FLARSSEDQNVFIVNDFQSPLQRTLSSLELPSSLPVASGQSVYPLDGGSSSENQRRTSSD 188 Query: 243 VRHGATSSMSQYSSKQNHKSNDPGRDLSIQVLEKFSLVTKFARDTTSSLFRDNSG 79 V + SS+SQ S + KS+DP RDLSI +LEKFSLVTKFARDTT+ LF +N+G Sbjct: 189 VGN-RVSSVSQ-SGFRKQKSHDPTRDLSIHLLEKFSLVTKFARDTTTQLFSENNG 241 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,636,083 Number of Sequences: 27288 Number of extensions: 303252 Number of successful extensions: 782 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 763 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 781 length of database: 11,516,596 effective HSP length: 96 effective length of database: 8,896,948 effective search space used: 1192191032 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179147|gb|BM380357.1|BM380357 MEST518-G06.univ ISUM6 Zea mays cDNA clone MEST518-G06 3', mRNA sequence (675 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g05060.1 expressed protein / ;supported by full-length cDN... 78 4e-15 >At1g05060.1 expressed protein / ;supported by full-length cDNA: Ceres:37455. Length = 253 Score = 78.2 bits (191), Expect = 4e-15 Identities = 37/77 (48%), Positives = 48/77 (62%) Frame = -2 Query: 671 LKAVIAGYSQIGKAFITATFMVFGGATAVLLYTADKLQLHSVDDVKTKGKDALQPRADMI 492 LKA G IGKAF AT ++FG AT V A KL + + DD++TKGKD QP+ + + Sbjct: 149 LKAAGEGLQHIGKAFAAATIIIFGSATLVFGTAASKLDMRNADDIRTKGKDLFQPKLESM 208 Query: 491 KEQIAPLRSWVRVRSDK 441 KEQ+ PLR+W S K Sbjct: 209 KEQVEPLRTWAENMSKK 225 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,117,067 Number of Sequences: 27288 Number of extensions: 226452 Number of successful extensions: 604 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 592 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 604 length of database: 11,516,596 effective HSP length: 96 effective length of database: 8,896,948 effective search space used: 1138809344 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179130|gb|BM380340.1|BM380340 MEST518-E06.univ ISUM6 Zea mays cDNA clone MEST518-E06 3', mRNA sequence (478 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done ***** No hits found ****** Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,157,204 Number of Sequences: 27288 Number of extensions: 75894 Number of successful extensions: 275 Number of sequences better than 1.0e-05: 0 Number of HSP's better than 0.0 without gapping: 262 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 275 length of database: 11,516,596 effective HSP length: 91 effective length of database: 9,033,388 effective search space used: 605236996 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179124|gb|BM380334.1|BM380334 MEST518-D10.univ ISUM6 Zea mays cDNA clone MEST518-D10 3', mRNA sequence (716 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g04910.1 putative protein / similar to unknown protein (db... 124 5e-29 >At5g04910.1 putative protein / similar to unknown protein (dbj|BAA90353.1) Length = 217 Score = 124 bits (311), Expect = 5e-29 Identities = 65/132 (49%), Positives = 85/132 (64%) Frame = -1 Query: 713 EFNDCSKQVLEIISLLSMPEFSRSDLADLLKGVQAHEKEKLHLTAKIQVLKKAGRPSERL 534 EF++CSKQV E+ SL PE R+DLA LL +Q EK+KLHL VLKKAGRPSER+ Sbjct: 91 EFSECSKQVREMESLFLNPEVGRADLAKLLSDIQTQEKQKLHL-----VLKKAGRPSERM 145 Query: 533 VNHADCRSRSMAQHVCVHVKEITXXXXXXXXXXXXXXXXXXXXAIQGVQVAVSSINEHME 354 + H +C+ + QH CVH+ EIT AI+GVQ AV+SINE++E Sbjct: 146 LTHENCKFKKPMQHECVHLHEITEAAGTEEAEADAEFDNALKEAIRGVQDAVTSINEYLE 205 Query: 353 EVRYEIDGLEAE 318 ++RYEI+ LEA+ Sbjct: 206 DIRYEIEALEAD 217 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,408,332 Number of Sequences: 27288 Number of extensions: 226762 Number of successful extensions: 736 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 713 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 735 length of database: 11,516,596 effective HSP length: 96 effective length of database: 8,896,948 effective search space used: 1263366616 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18179095|gb|BM380305.1|BM380305 MEST518-A09.univ ISUM6 Zea mays cDNA clone MEST518-A09 3', mRNA sequence (673 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g28140.1 expressed protein / contains similarity to cytoch... 201 4e-52 >At1g28140.1 expressed protein / contains similarity to cytochrome oxidase I GI:1289267 from [Xantholinus sp.];supported by full-length cDNA: Ceres:6875. Length = 280 Score = 201 bits (510), Expect = 4e-52 Identities = 94/141 (66%), Positives = 114/141 (80%) Frame = -3 Query: 671 QALWAAGALGSVGTYIVAARPLDEGLVQYVLDHTAALWFVGPTFAALTGLVFKEGLCYGK 492 QALWA G +GS TY ARP + LV YV+DH +A+WFVGP FA+LTGLVFKEGLCYGK Sbjct: 138 QALWALGFVGSFATYAALARPAGDNLVHYVVDHPSAVWFVGPLFASLTGLVFKEGLCYGK 197 Query: 491 LEAGILTFLIPGLLLGHLSGLMDNSTKAGLLGLWMLLFTIFAARKFQQPIKDDVGDKSVF 312 LEAG+LTF+IP +LLGHLSGLM++ K LLG WM LF +FA RKF QPIKDD+GDKSVF Sbjct: 198 LEAGLLTFIIPSVLLGHLSGLMNDEVKLVLLGTWMALFLVFAGRKFTQPIKDDIGDKSVF 257 Query: 311 MFNALPEEEKKAWIQKLERQK 249 F +L ++EKKA ++KLE++K Sbjct: 258 TFMSLSDDEKKAIVEKLEQEK 278 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,189,802 Number of Sequences: 27288 Number of extensions: 274508 Number of successful extensions: 894 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 857 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 892 length of database: 11,516,596 effective HSP length: 96 effective length of database: 8,896,948 effective search space used: 1129912396 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18178992|gb|BM380202.1|BM380202 MEST516-E06.univ ISUM6 Zea mays cDNA clone MEST516-E06 3', mRNA sequence (645 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g65270.1 expressed protein / ;supported by full-length cDN... 182 1e-46 >At1g65270.1 expressed protein / ;supported by full-length cDNA: Ceres:6906. Length = 292 Score = 182 bits (462), Expect = 1e-46 Identities = 79/119 (66%), Positives = 103/119 (86%) Frame = -1 Query: 612 SSIRARCIPRDSLDEHIVIHMDGVNILAVNYGSVDGCQYPRPMKLPSKWTFSSYTILKTA 433 +S+RARC+PRD LDEH +IHM+G NILAV+YGS CQYPR +KLP+KW+F+S+TILK++ Sbjct: 156 ASVRARCLPRDGLDEHFIIHMEGANILAVSYGSPGACQYPRQLKLPAKWSFNSHTILKSS 215 Query: 432 EQAPRTPSFADQLIEADNGLGEVMKPPEKSFWAKYWMYIIPLGLIVMNAVTAAANIPEE 256 EQAPRTP F ++++ ++N GEV PPE+SFWAKYWMY+IPLGL+VMNAVT A+N+ EE Sbjct: 216 EQAPRTPIFTEEILGSENVEGEVEPPPERSFWAKYWMYLIPLGLVVMNAVTQASNMAEE 274 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,410,370 Number of Sequences: 27288 Number of extensions: 271108 Number of successful extensions: 718 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 697 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 717 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 1061984084 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18178976|gb|BM380186.1|BM380186 MEST516-C07.univ ISUM6 Zea mays cDNA clone MEST516-C07 3', mRNA sequence (664 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g26550.1 expressed protein / contains similarity to peptid... 220 6e-58 At2g18040.1 putative peptidyl-prolyl cis-trans isomerase / si... 53 2e-07 >At1g26550.1 expressed protein / contains similarity to peptidyl-prolyl cis/trans isomerase GB:AAC62692 GI:3599386 from [Cenarchaeum symbiosum];supported by full-length cDNA: Ceres:14278. Length = 142 Score = 220 bits (560), Expect = 6e-58 Identities = 100/110 (90%), Positives = 104/110 (93%) Frame = -3 Query: 539 SADGLGTCTYVKARHVLCEKQGKINEAYKKLQDGWLNNGDKVPPAEFAKVAQEYSECPSG 360 +ADGLGTCTYVKARHVLCEKQGKINEAYKKLQDGWL+NGDKVPPAEFAK+A EYSECPSG Sbjct: 33 AADGLGTCTYVKARHVLCEKQGKINEAYKKLQDGWLSNGDKVPPAEFAKIAAEYSECPSG 92 Query: 359 KKGGDLGWFPRGKMAGPFQEVAFNTPEGTVSAPFKSTHGYHFILCEGRKN 210 KKGGDLGWFPRGKMAGPFQ+VAFNTP G SAPFKSTHGYH IL EGRKN Sbjct: 93 KKGGDLGWFPRGKMAGPFQDVAFNTPVGVTSAPFKSTHGYHIILSEGRKN 142 >At2g18040.1 putative peptidyl-prolyl cis-trans isomerase / similar to ESS1 (S.cerevisiae) and dodo (D.melanogaster.); supported by full-length cDNA: Ceres: 12602. Length = 119 Score = 52.8 bits (125), Expect = 2e-07 Identities = 37/109 (33%), Positives = 50/109 (44%), Gaps = 16/109 (14%) Frame = -3 Query: 509 VKARHVLCEKQGKINEAYKKLQDGWLN---------------NGDKVP-PAEFAKVAQEY 378 VKA H+L + QG +A K +G + D V A F +VA Sbjct: 7 VKASHILIKHQGSRRKASWKDPEGKIILTTTREAAVEQLKSIREDIVSGKANFEEVATRV 66 Query: 377 SECPSGKKGGDLGWFPRGKMAGPFQEVAFNTPEGTVSAPFKSTHGYHFI 231 S+C S K+GGDLG F RG+M PF+E + G +S + G H I Sbjct: 67 SDCSSAKRGGDLGSFGRGQMQKPFEEATYALKVGDISDIVDTDSGVHII 115 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,697,375 Number of Sequences: 27288 Number of extensions: 284774 Number of successful extensions: 860 Number of sequences better than 1.0e-05: 4 Number of HSP's better than 0.0 without gapping: 837 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 860 length of database: 11,516,596 effective HSP length: 96 effective length of database: 8,896,948 effective search space used: 1103221552 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18178973|gb|BM380183.1|BM380183 MEST516-C02.univ ISUM6 Zea mays cDNA clone MEST516-C02 3', mRNA sequence (618 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g23395.1 unknown protein / predicted by genemarkHMM 117 6e-27 >At5g23395.1 unknown protein / predicted by genemarkHMM Length = 162 Score = 117 bits (292), Expect = 6e-27 Identities = 52/76 (68%), Positives = 62/76 (81%), Gaps = 1/76 (1%) Frame = -3 Query: 613 SFDEDDTE-ESIEMKVQKALDCPCVADLKNGPCGGQFVDAFSCFLRSTEEEKGSDCVKPF 437 ++ EDD E ES+E K Q+ALDCPC+ADL+NG CG QF +AF CFL+ST EEKGSDCV PF Sbjct: 47 AYGEDDNENESLEAKAQRALDCPCIADLRNGSCGSQFSEAFLCFLKSTAEEKGSDCVNPF 106 Query: 436 ITLQDCIKANPEAFSK 389 + LQ CI ANP+AFSK Sbjct: 107 VALQSCINANPDAFSK 122 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,091,624 Number of Sequences: 27288 Number of extensions: 242109 Number of successful extensions: 565 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 548 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 563 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 981665960 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18178613|gb|BM379823.1|BM379823 MEST511-A10.univ ISUM6 Zea mays cDNA clone MEST511-A10 3', mRNA sequence (422 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g39630.1 expressed protein / supported by full-length cDNA... 84 4e-17 >At4g39630.1 expressed protein / supported by full-length cDNA: Ceres:21166. Length = 233 Score = 83.6 bits (205), Expect = 4e-17 Identities = 42/64 (65%), Positives = 48/64 (74%), Gaps = 1/64 (1%) Frame = -3 Query: 360 EGQMAGASEAFQTPGAVSNRLSCGMTPKTVRLPKNGEMLLSVHGSPLGVYKEE-NLAAIE 184 E QM +AFQTPG RLS GMTPKT+RLPK GEM+LSVHGSPLGVYKE+ N+ AI Sbjct: 170 ENQMLPGHDAFQTPGVNGQRLSFGMTPKTLRLPKAGEMMLSVHGSPLGVYKEDHNMGAIN 229 Query: 183 ESGN 172 E + Sbjct: 230 EENS 233 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,708,132 Number of Sequences: 27288 Number of extensions: 179706 Number of successful extensions: 395 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 388 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 395 length of database: 11,516,596 effective HSP length: 89 effective length of database: 9,087,964 effective search space used: 463486164 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18178362|gb|BM379572.1|BM379572 MEST507-D07.univ ISUM6 Zea mays cDNA clone MEST507-D07 3', mRNA sequence (568 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g50900.1 expressed protein / ;supported by full-length cDN... 88 4e-38 >At1g50900.1 expressed protein / ;supported by full-length cDNA: Ceres:19056. Length = 175 Score = 87.8 bits (216), Expect(2) = 4e-38 Identities = 43/60 (71%), Positives = 53/60 (87%), Gaps = 1/60 (1%) Frame = -2 Query: 432 HPVDILLMLAASEGDRPKIEELLRAGARFDVKDVDGRTALDRA-TDETREFILGFAAKKA 256 HPVDILLMLAA+EGDRPKIEELL+AGA + VKD DGRTA+DRA ++E R+ ILG++ +KA Sbjct: 116 HPVDILLMLAATEGDRPKIEELLKAGADYSVKDADGRTAIDRANSEEIRDLILGYSTQKA 175 Score = 87.4 bits (215), Expect(2) = 4e-38 Identities = 41/46 (89%), Positives = 43/46 (93%) Frame = -1 Query: 565 AGIYGSQARDDFDRDDVEQYFNYMGMLAVEGTYDKMEALLNQDIPP 428 AGIYGSQ+RDDFDRDDVEQYFNYMGMLAVEGTY KMEALLN +I P Sbjct: 72 AGIYGSQSRDDFDRDDVEQYFNYMGMLAVEGTYSKMEALLNLNIHP 117 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,270,339 Number of Sequences: 27288 Number of extensions: 203517 Number of successful extensions: 744 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 694 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 744 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 841443256 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18178357|gb|BM379567.1|BM379567 MEST507-C10.univ ISUM6 Zea mays cDNA clone MEST507-C10 3', mRNA sequence (565 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g73885.1 hypothetical protein / predicted by genemarkHMM 99 2e-21 >At1g73885.1 hypothetical protein / predicted by genemarkHMM Length = 192 Score = 98.6 bits (244), Expect = 2e-21 Identities = 46/84 (54%), Positives = 57/84 (67%), Gaps = 2/84 (2%) Frame = -3 Query: 533 PDFWEGPQWEALGFFVQYMWAFGVVFGLIACGVAVATYNDGATDFKDTPAYKXX--XXXX 360 PD +EG +W+ LGFFVQY+WAFG++F LI+ G+A TYN+GATDFK+TP YK Sbjct: 96 PDPFEGEKWDGLGFFVQYLWAFGILFALISGGLAAGTYNEGATDFKETPVYKEAIQSRDL 155 Query: 359 XXXXXXXXSADVFEGNPTEVAPAL 288 S DVFE NPTEVAP + Sbjct: 156 LDEAESSNSEDVFESNPTEVAPTI 179 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,731,125 Number of Sequences: 27288 Number of extensions: 234876 Number of successful extensions: 716 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 699 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 715 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 832491732 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18178307|gb|BM379517.1|BM379517 MEST506-F07.univ ISUM6 Zea mays cDNA clone MEST506-F07 3', mRNA sequence (636 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g22120.1 unknown protein / ; supported by cDNA: gi_20147369 131 3e-31 >At5g22120.1 unknown protein / ; supported by cDNA: gi_20147369 Length = 383 Score = 131 bits (330), Expect = 3e-31 Identities = 68/123 (55%), Positives = 87/123 (70%), Gaps = 1/123 (0%) Frame = -3 Query: 634 RIFEKFGDHGVAIRWINDTSALAVFRTPSAANEAQACIPPRYKVRSLKPDDDLLTKIDGR 455 ++F+ F D G IRW+NDT+ALAVF+TPSAA EA + + +R L +D LL I G+ Sbjct: 262 KLFKDFKDSGFIIRWVNDTTALAVFKTPSAALEACKHVQCSFTIRVLDDNDSLLGSISGK 321 Query: 454 DLDPPKPRPKTSARTAQRLIAHGMGLK-QFTNFGSDELKKQEGERKDRIAARQAMRDEAW 278 DL+PP RPKTSA+TAQRLIAH MGLK + FGS E + QE RK+RI +RQ R++AW Sbjct: 322 DLEPPSQRPKTSAKTAQRLIAHSMGLKLPASGFGSKE-RDQEAARKNRIVSRQKQREDAW 380 Query: 277 GSD 269 G D Sbjct: 381 GDD 383 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,986,413 Number of Sequences: 27288 Number of extensions: 270669 Number of successful extensions: 786 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 761 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 786 length of database: 11,516,596 effective HSP length: 95 effective length of database: 8,924,236 effective search space used: 1035211376 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18178248|gb|BM379458.1|BM379458 MEST505-H03.univ ISUM6 Zea mays cDNA clone MEST505-H03 3', mRNA sequence (565 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g05620.1 expressed protein / ;supported by full-length cD... 151 3e-37 >At2g05620.1 expressed protein / ;supported by full-length cDNA: Ceres:1618. Length = 133 Score = 151 bits (381), Expect = 3e-37 Identities = 76/110 (69%), Positives = 87/110 (79%) Frame = -3 Query: 458 WSSSVSGDDHHRSYSAALAMSVSVRPRCRARSPARMMGNVNAGKGLFAPLVVVARNIIGR 279 W SS+SG+D+ S +A R +A MM NVN GKGLFAPLVVV RN++G+ Sbjct: 24 WGSSISGEDYQTMLSKTVAPPQQARVSRKAIRAVPMMKNVNEGKGLFAPLVVVTRNLVGK 83 Query: 278 KRFNQLRGKAIALHSQVITEFCKTIGADSKQRQGLIRLAKKNGEKLGFLA 129 KRFNQLRGKAIALHSQVITEFCK+IGAD+KQRQGLIRLAKKNGE+LGFLA Sbjct: 84 KRFNQLRGKAIALHSQVITEFCKSIGADAKQRQGLIRLAKKNGERLGFLA 133 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,574,328 Number of Sequences: 27288 Number of extensions: 149708 Number of successful extensions: 597 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 585 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 596 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 832491732 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18178105|gb|BM379315.1|BM379315 MEST503-H05.univ ISUM6 Zea mays cDNA clone MEST503-H05 3', mRNA sequence (568 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g29735.1 Expressed protein / ; supported by full-length cD... 77 5e-15 >At4g29735.1 Expressed protein / ; supported by full-length cDNA: Ceres: 26443. Length = 76 Score = 77.4 bits (189), Expect = 5e-15 Identities = 40/76 (52%), Positives = 52/76 (67%) Frame = -3 Query: 440 MAAKAISSPVPVEWYPTLAVVMVSVGLMFTASFFIYEATSSRRSRSLSKEIATAVVAXXX 261 MAAK I+SP+PV YPTL+V +++GL+ TA FFIYEATSSR++RS+ KE+AT+ VA Sbjct: 1 MAAKPIASPIPVALYPTLSVFTLAIGLVITAIFFIYEATSSRKNRSVGKELATSAVASVF 60 Query: 260 XXXXXXXXXXXXGVYV 213 GVYV Sbjct: 61 LGFGSLFLLLASGVYV 76 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,102,383 Number of Sequences: 27288 Number of extensions: 202932 Number of successful extensions: 559 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 539 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 555 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 841443256 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18178047|gb|BM379257.1|BM379257 MEST503-A05.univ ISUM6 Zea mays cDNA clone MEST503-A05 3', mRNA sequence (562 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g42850.1 expressed protein / ;supported by full-length cD... 125 1e-29 >At5g42850.1 expressed protein / ;supported by full-length cDNA: Ceres:23293. Length = 134 Score = 125 bits (314), Expect = 1e-29 Identities = 59/109 (54%), Positives = 72/109 (65%) Frame = -3 Query: 503 DAASDGKVKLLLFLADRKPGSSLSWCPDCNVAEPVIYERLEALEGKDAVLLRAYVGDKPT 324 D S K+ +LFLAD P + SWCPDC AEPVIY+ LE ++ L+RAY GD+PT Sbjct: 23 DETSRSKINFILFLADNDPTTGQSWCPDCVRAEPVIYKTLEEFP-EEVKLIRAYAGDRPT 81 Query: 323 WRDPAHPWRVDPRFGLKGVPTLIRWEDGAAAARLGDDEAHLKDKVDALL 177 WR PAHPWRVD RF L GVPTL+RW+ + RL D +AHL + LL Sbjct: 82 WRTPAHPWRVDSRFKLTGVPTLVRWDGDSVKGRLEDHQAHLPHLILPLL 130 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,508,698 Number of Sequences: 27288 Number of extensions: 245584 Number of successful extensions: 989 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 895 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 981 length of database: 11,516,596 effective HSP length: 93 effective length of database: 8,978,812 effective search space used: 835029516 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18178045|gb|BM379255.1|BM379255 MEST502-H12.univ ISUM6 Zea mays cDNA clone MEST502-H12 3', mRNA sequence (601 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g42320.1 unknown protein / 169 8e-43 >At5g42320.1 unknown protein / Length = 461 Score = 169 bits (429), Expect = 8e-43 Identities = 83/145 (57%), Positives = 109/145 (74%) Frame = -3 Query: 446 SDSLLREIKALVARHSSKLSMDTIKTSNKGYSAELFVVTFNHVKERMDNGSKVHILLSFG 267 SD L+ +I +LV RH KLS++ IK+ NKGY+AE+ VVT+ + D+ S ILL+FG Sbjct: 79 SDDLMEQIHSLVHRHPDKLSIELIKSGNKGYNAEVNVVTYCRGGKESDDRSNFRILLTFG 138 Query: 266 QHGRELITSEVALRLLYVLTEKPKIAGVDISSFEKLLENLVIKVVPMENMNGRKRVEEGE 87 QHGRELITSE+A R+L +L+E+ + + + L+ LVIK+VP+EN NGRKRVE G+ Sbjct: 139 QHGRELITSELAFRILSILSEEQFLPNKNGGILKNTLDKLVIKMVPIENPNGRKRVESGD 198 Query: 86 LCDRRNGRGVDLNRNWSVDWGKKKK 12 LC+RRNGRGVDLNRNW VDWGKK+K Sbjct: 199 LCERRNGRGVDLNRNWGVDWGKKEK 223 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,001,870 Number of Sequences: 27288 Number of extensions: 244803 Number of successful extensions: 611 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 600 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 611 length of database: 11,516,596 effective HSP length: 94 effective length of database: 8,951,524 effective search space used: 939910020 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= gi|18177921|gb|BM379131.1|BM379131 MEST500-B01.univ ISUM6 Zea mays cDNA clone MEST500-B01 3', mRNA sequence (500 letters) Database: ../ATH1_TIGR.pep 27,288 sequences; 11,516,596 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g22550.1 putative protein / predicted to predicted protein... 75 2e-14 >At4g22550.1 putative protein / predicted to predicted protein, C.elegans Length = 213 Score = 74.7 bits (182), Expect = 2e-14 Identities = 47/108 (43%), Positives = 58/108 (53%), Gaps = 28/108 (25%) Frame = -3 Query: 489 MYVAVSADHWSFPSGHSSRAFLVAS----FLAA--------------GGFRPRE------ 382 M AVSADH+SFPSGH+SR F VA+ F AA G R Sbjct: 99 MSAAVSADHYSFPSGHASRVFFVAASVHFFSAAAEASMTGPSYSFLDGWIRDHNDGDVKV 158 Query: 381 ----ALFLWAAATSASRVLLGRHYVLDVVAGACLGVFEAWLSILLLGF 250 +++WA T+ SR+LLGRHYVLDV AGA LG+ EA ++ L F Sbjct: 159 EVVVVVWIWATVTAISRILLGRHYVLDVAAGAFLGIVEALFALRFLRF 206 Database: ../ATH1_TIGR.pep Posted date: Aug 20, 2002 9:10 AM Number of letters in database: 11,516,596 Number of sequences in database: 27,288 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,855,267 Number of Sequences: 27288 Number of extensions: 133119 Number of successful extensions: 384 Number of sequences better than 1.0e-05: 2 Number of HSP's better than 0.0 without gapping: 372 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 383 length of database: 11,516,596 effective HSP length: 92 effective length of database: 9,006,100 effective search space used: 666451400 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) BLASTX 2.2.3 [Apr-24-2002] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs",